2023
DOI: 10.3390/curroncol30060395
|View full text |Cite
|
Sign up to set email alerts
|

Novel Tools for Diagnosis and Monitoring of AML

Abstract: In recent years, major advances in the understanding of acute myeloid leukemia (AML) pathogenesis, together with technological progress, have led us into a new era in the diagnosis and follow-up of patients with AML. A combination of immunophenotyping, cytogenetic and molecular studies are required for AML diagnosis, including the use of next-generation sequencing (NGS) gene panels to screen all genetic alterations with diagnostic, prognostic and/or therapeutic value. Regarding AML monitoring, multiparametric … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 9 publications
(2 citation statements)
references
References 64 publications
0
2
0
Order By: Relevance
“…UMI-based error-corrected NGS has better sensitivity but still generally up to 0.1% VAF, i.e., ~1 log less than qPCR-based techniques [29]. While having the advantage of assessing a variety of genes in a single test, this type of NGS requires the use of expensive equipment and highly specialized bioinformatics tools, both being not widely available [30].…”
Section: Discussionmentioning
confidence: 99%
“…UMI-based error-corrected NGS has better sensitivity but still generally up to 0.1% VAF, i.e., ~1 log less than qPCR-based techniques [29]. While having the advantage of assessing a variety of genes in a single test, this type of NGS requires the use of expensive equipment and highly specialized bioinformatics tools, both being not widely available [30].…”
Section: Discussionmentioning
confidence: 99%
“…Also, the combinations of markers for AML MRD detection varies between different institutions, resulting in different operator-dependent gating strategies. Furthermore, all examiners require a high level of expertise in the interpretation of MFC-based MRD analysis [ 28 , 40 ]. Further limitations of the method include immunophenotypic shifts at relapse due to the possible evolution of subclones, which increases the difficulty of detecting MRD [ 37 ].…”
Section: Current Methods Of Mrd Detectionmentioning
confidence: 99%