2011
DOI: 10.4238/vol10-2gmr1059
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Novel transcripts of human cytomegalovirus clinical strain found by cDNA library screening

Abstract: ABSTRACT. Human cytomegalovirus (HCMV) is a double-stranded DNA virus with the largest genome (~235 kb) of the known human herpes viruses. The coding potential and transcript structures of most HCMV predicted genes have not been identified. New or unknown genes could exist in clinical strains. The SMART (switching mechanism at 5ꞌ end of RNA template of reverse transcriptase) technique was used to construct a full-length cDNA library of an HCMV clinical strain in the late expression phase. Randomly selected clo… Show more

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Cited by 19 publications
(17 citation statements)
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“…A cDNA library of the HCMV clinical strain H had been previously constructed using the pBluescript II SK vector [24]. The primary library consisted of 1.12 × 10 6 recombinant clones/mL.…”
Section: Methodsmentioning
confidence: 99%
“…A cDNA library of the HCMV clinical strain H had been previously constructed using the pBluescript II SK vector [24]. The primary library consisted of 1.12 × 10 6 recombinant clones/mL.…”
Section: Methodsmentioning
confidence: 99%
“…RNA2.7 functions in inhibiting apoptosis (13), the RNA5.0 homolog in murine cytomegalovirus is involved in virulence (14), and the role of RNA1.2 is unknown. Antisense transcripts (ASTs) form a second class of noncoding RNA, and are transcribed antisense to CRs (15,16). In addition, HCMV specifies several small, nonpolyA RNAs that may have regulatory roles, including microRNAs (miRNAs) (17) processed from longer transcripts and RNAs associated with the origin of DNA replication (18).…”
mentioning
confidence: 99%
“…In this study, the transcription of the AS strand of the HCMV UL87 gene area was investigated, and an 800 nt UL87 AS transcript was deeply characterized, which has been found as a cDNA clone in a late HCMV cDNA library [14]. The transcript was identified in three HCMV clinical strains.…”
Section: Discussionmentioning
confidence: 99%
“…However, two AS transcripts overlapping the UL87 gene were obtained by screening a HCMV cDNA library made during late infection, in the study by Zhang et al [10]. Moreover, we also found two cDNA clones in a late HCMV cDNA library containing the sequence of the UL87 AS strand [14]. …”
Section: Introductionmentioning
confidence: 98%