Novel transformations of i-cholesterol and 6β-methoxy-i-cholesterol by Moraxella Sp.

Rao, Mamatha K.; Madyastha, K. M.; Bhattacharyya, P.

doi:10.1016/0022-4731(83)91112-3

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…was confirmed by Marsheck et al (1972), Conner et al (1976, Srivastava et al (1985), Hesselink et al (1989), Liu and Lo (1990), Lee and Liu (1992), Seidel and Hörhold (1992), Zhang et al (1992), Lee et al (1993), Smith et al (1993), Weber et al (1995a, b), Liu and Lo (1997), Dias et al (2002), Pérez et al (2005). Very rare work was found on, the selective degradation of cholesterol and phytosterols by Genus Moraxella (Rao et al 1983;Madyastha and Shanker 1994).…”

Section: Synthetic Mediasupporting

confidence: 57%

Microbial transformation of phytosterols mixture from rice bran oil unsaponifiable matter by selected bacteria

Sallam

Osman

Hamdy

et al. 2008

World J Microbiol Biotechnol

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Rice bran sample (12 Kg) was extracted and rice bran oil (RBO % 76.8 g) was saponified. The resulted unsaponifiable matter of RBO (RBO unsap) was qualitatively and quantitatively estimated using different chromatographic analyses. RBO, produced 9.65% unsaponifiable matter with the following contents, cholesterol, 6.75%; stigmasterol, 3.4%; b. sitosterol, 10.23% and campesterol, 4.2%, in addition to unknown phytosterols, hydrocarbons and waxes. Microbial transformation process started by screening of 35 bacterial strains, locally isolated from rice bran, air and soil, using RBO unsap as a carbon and an energy source to produce some pharmaceutically useful C 18 and C 19 steroids. Moraxella ovis was the most potent isolate for its highest capability to utilize RBO unsap and selectively degrade the phytosterols side-chain producing androst-4-ene-3,17-dione (AD), androsta-1,4-diene-3,17-dione (ADD), testosterone (T) and estrone (E). The RBO unsap was the best carbon and energy source. Maximum production of the desired products was observed in 36 h, pH 7 and at 30°C by M. ovis.

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Section: Synthetic Mediasupporting

confidence: 57%

Microbial transformation of phytosterols mixture from rice bran oil unsaponifiable matter by selected bacteria

Sallam

Osman

Hamdy

et al. 2008

World J Microbiol Biotechnol

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Evidence for a new pathway in the microbial conversion of 3β-acetoxycholest-5-en-19-ol into estrone

Shankar¹,

Row²,

Madyastha³

1993

J. Chem. Soc., Perkin Trans. 1

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Role of Neutral Metabolites in Microbial Conversion of 3β-Acetoxy-19-Hydroxycholest-5-Ene into Estrone

Madyastha

Shankar

1994

Appl Environ Microbiol

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Biotransformation of 3,-acetoxy-19-hydroxycholest-5-ene (19-HCA, 6 g) by Moraxella sp. was studied. Estrone (712 mg) was the major metabolite formed. Minor metabolites identified were 5a-androst-1-en-19-ol-3,17-dione (33 mg), androst-4-en-19-ol-3,17-dione (58 mg), androst-4-en-9a,19-diol-3,17-dione (12 mg), and androstan-19-ol-3,17-dione (1 mg). Acidic metabolites were not formed. Time course experiments on the fermentation of 19-HCA indicated that androst-4-en-19-ol-3,17-dione was the major metabolite formed during the early stages of incubation. However, with continuing fermentation its level dropped, with a concomitant increase in estrone. Fermentation of 19-HCA in the presence of specific inhibitors or performing the fermentation for a shorter period (48 h) did not result in the formation of acidic metabolites. Resting-cell experiments carried out with 19-HCA (200 mg) in the presence of a,a'-bipyridyl led to the isolation of three additional metabolites, viz., cholestan-19-ol-3-one (2 mg), cholest-4-en-19-ol-3-one (10 mg), and cholest-5-en-3jS,19-diol (12 mg). Similar results were also obtained when n-propanol was used instead of a,o'-bipyridyl. Resting cells grown on 19-HCA readily converted both 5G-androst-1-en-19-ol-3,17-dione and androst-4-en-19

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Novel transformations of i-cholesterol and 6β-methoxy-i-cholesterol by Moraxella Sp.

Cited by 3 publications

References 5 publications

Microbial transformation of phytosterols mixture from rice bran oil unsaponifiable matter by selected bacteria

Microbial transformation of phytosterols mixture from rice bran oil unsaponifiable matter by selected bacteria

Evidence for a new pathway in the microbial conversion of 3β-acetoxycholest-5-en-19-ol into estrone

Role of Neutral Metabolites in Microbial Conversion of 3β-Acetoxy-19-Hydroxycholest-5-Ene into Estrone

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