Novel transparent collagen film patch derived from duck’s feet for tympanic membrane perforation

Abstract

To increase healing rate of tympanic membrane (TM) perforations, patching procedure has been commonly conducted. Biocompatible, biodegradable patching materials which is not limited across cultures is needed. The authors evaluated the effectiveness of novel transparent duck's feet collagen film (DCF) patch in acute traumatic TM perforation. This procedure was compared with spontaneous healing and paper patching. Cell proliferation features were observed in paper and DCF patches. Forty-eight TMs of 24 rats were used for animal experiment, perforations were made on each TMs, and divided into three groups according to treatment modality. Sixteen were spontaneously healed, 16 were paper patched and 16 were DCF patched. The gross and histological healing results were analyzed. Both paper and DCF patch showed no cytotoxicity, but cell proliferations were more active in DCF than paper in early stage. In animal study, the healing of TM perforations were completed within 14 days in all three groups, but found to be faster in DCF patch group than paper patch or spontaneous healing group. The DCF patches were transparent and size of DCF patches were gradually decreased, so there were no need to remove the DCF patches to check the wound status or after the completion of healing. According to this result, authors concluded that DCF patch is transparent, biocompatible and biodegradable material, and can induce fast healing in acute traumatic TM perforations.

Human Acellular Dermal Allograft Patch on Traumatic Tympanic Membrane Perforation

“…Before the 1950s, the modern era of middle-ear surgery including myringoplasty and tympanoplasty using microscopes, Blake introduced the first paper-patch graft technique for the treatment of tympanic membrane perforation in 1887, 29 and this technique is still widely used to treat acute and TTMP. In recent years, various patch materials such as eggshell membrane, 10 silk, 9 gel foam, 11 collagen film, 8 Steri-Strips(3M ® ), 7 and calcium alginate 6 , 30 have been reported. 31 Paper patches act as scaffolds inducing epithelial growth over the attached patches, with high success rates, but limited to the cases with small-sized perforation.…”

Soft, environmentally degradable microfluidic devices for measurement of sweat rate and total sweat loss and for colorimetric analysis of sweat biomarkers

“…The field of bioresorbable electronics, as a subset of the broader area of transient electronics, relies on a range of materials for substrates and encapsulation layers, including silk fibroin, 23,24 wax, 25 collagen, 26 poly(lactic-co-glycolic acid) (PLGA), 27,28 polyanhydride, 29 polycaprolactone (PCL), 30,31 and poly(1,8-octanediol-co-citrate) (POC). 32 Each of these materials dissolves completely and harmlessly by hydrolysis in biofluids within weeks or months, but none satisfies all of the requirements listed above and most are not currently in mass production.…”

Necessities, opportunities, and challenges for tympanic membrane perforation scaffolding-based bioengineering

“…The bacterial cellulose-based fibrillary scaffolds exhibit a transparent nanostructure, and It holds the potential to accelerate the regeneration of acute TMP [142]. The duck feet derived collagen film is biocompatible, biodegradable, transparent, and showed better healing of acute perforation (SD rats; n = 16) than the paper patch [136].…”

“…In particular, the polymer-based patch could be transparent and can provide opportunities to see healing progress and middle ear pathologies. These scaffolds provide physical support to bridge the perforation gab and accelerate cellular response [136,142,146].…”