Novel trichothecanes, paecilomycine A, B, and C, isolated from entomopathogenic fungus, Paecilomyces tenuipes

Kikuchi, Haruhisa; Miyagawa, Yasuhiro; Sahashi, Yuko; Inatomi, Satoshi; Haganuma, Asami; Nakahata, Norimichi; Oshima, Yoshiteru

doi:10.1016/j.tetlet.2004.06.107

Cited by 48 publications

(35 citation statements)

References 8 publications

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“…silkworm) or solid media are used for commercial products. As for low molecular weight metabolites from this fungus, a group of trichothecanes, paecilomycine A, 18 tenuipesines A and B, 19 and spirotenuipesines A-C, 20 have been isolated from the synnemata grown on silkworms, wherebeauvericin *To whom correspondence should be addressed. E-mail: isaka@biotec.or.th as a pseudodipeptide, hanasanagin (3,4-diguanidino-butanoyl-DOPA) was isolated by other group.…”

Section: Introductionmentioning

confidence: 99%

Beauvericin production by the Lepidoptera pathogenic fungus Isaria tenuipes: Analysis of natural specimens, synnemata from cultivation, and mycelia from liquid-media fermentation

Supothina

Srisanoh

Nithithanasilp

et al. 2011

Nat. Prod. Bioprospect.

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Beauvericin was analyzed in three forms of the Lepidoptra pathogenic fungus Isaria tenuipes (4 isolates): (a) natural specimen, (b) cultivated synnemata on rice media, and (c) mycelia from fermentation in liquid media. Beauvericin was detected in very low amounts in all tested natural specimens. Synnemata on rice contained much higher concentrations of beauvericin than the corresponding natural materials, although the concentrations were lower than mycelia from liquid fermentation. The results casted a caution that beauvericin concentration should be carefully checked, as a possible toxic constituent, upon mass production of a selected strain of Isaria tenuipes for health food purposes.

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Section: Introductionmentioning

confidence: 99%

Beauvericin production by the Lepidoptera pathogenic fungus Isaria tenuipes: Analysis of natural specimens, synnemata from cultivation, and mycelia from liquid-media fermentation

Supothina

Srisanoh

Nithithanasilp

et al. 2011

Nat. Prod. Bioprospect.

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“…For example, researchers have reported an efficient method for the identification of NGFregulatory molecules, which resulted in the identification of CAD (5, 19-cyclo-9 beta, 10 xi-androstane-3, 17-dione), complanadin B, and lyconadin B. [16][17][18] Kikuchi et al 16 also reported the successful evaluation of PC12 cellular elongation. In both of these studies, two types of cells (1321N1 and PC12) were used in the assay.…”

Section: Research-article2016mentioning

confidence: 99%

“…The greatest advantage of this cell-based assay is its ability to sensitively detect NGF functionality in the 1321N1 supernatant, which can be confirmed quantitatively even in a small number of responding cells. Several studies [16][17][18] have combined reverse transcription (RT)-PCR or enzymelinked immunosorbent assay (ELISA) with such phenotypic outcomes. However, although these assays provide quantitative results, their results are limited to confirmation of an intermediate biological response (e.g., mRNA expression or protein production of NGF).…”

Section: Research-article2016mentioning

confidence: 99%

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Morphological Evaluation of Nonlabeled Cells to Detect Stimulation of Nerve Growth Factor Expression by Lyconadin B

et al. 2016

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The success of drug development is greatly influenced by the efficiency of drug screening methods. Recently, phenotypebased screens have raised expectations, based on their proven record of identifying first-in-class drugs at a higher rate. Although fluorescence images are the data most commonly used in phenotype-based cell-based assays, nonstained cellular images have the potential to provide new descriptive information about cellular responses. In this study, we applied morphology-based evaluation of nonlabeled microscopic images to a phenotype-based assay. As a study case, we attempted to increase the efficiency of a cell-based assay for chemical compounds that induce production of nerve growth factor (NGF), using lyconadin B as a model compound. Because the total synthesis of lyconadin B was accomplished very recently, there is no well-established cell-based assay scheme for further drug screening. The conventional cell-based assay for evaluating NGF induction requires two types of cells and a total of 5 days of cell culture. The complexity and length of this assay increase both the risk of screening errors and the cost of screening. Our findings show that analysis of cellular morphology enables evaluation of NGF induction by lyconadin B within only 9 h.

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“…[4] The report described that compound 1 (paecilomycine A), at 10 nm, is capable of fostering neurite outgrowth in PC 12 cells, apparently by enhancing the expression levels of neurotrophic factors in previously conditioned human astrocytoma cells. Also noted was the claim that paecilomycine A (1) is substantially more active than scabronine G in enhancing NGF levels.…”

mentioning

confidence: 99%

Total Synthesis of Paecilomycine A

Min

2007

Angew Chem Int Ed

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Novel trichothecanes, paecilomycine A, B, and C, isolated from entomopathogenic fungus, Paecilomyces tenuipes

Cited by 48 publications

References 8 publications

Beauvericin production by the Lepidoptera pathogenic fungus Isaria tenuipes: Analysis of natural specimens, synnemata from cultivation, and mycelia from liquid-media fermentation

Beauvericin production by the Lepidoptera pathogenic fungus Isaria tenuipes: Analysis of natural specimens, synnemata from cultivation, and mycelia from liquid-media fermentation

Morphological Evaluation of Nonlabeled Cells to Detect Stimulation of Nerve Growth Factor Expression by Lyconadin B

Total Synthesis of Paecilomycine A

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