Novel Triclosan-Bound Hybrid-Silica Nanoparticles and their Enhanced Antimicrobial Properties

Makarovsky, Igor; Boguslavsky, Yonit; Alesker, Maria; Lellouche, Jonathan; Banin, Ehud; Lellouche, Jean‐Paul

doi:10.1002/adfm.201101557

Cited by 41 publications

(31 citation statements)

References 56 publications

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“…[ 2 ] We also reported that a close physical proximity/ contact, i.e., a direct interaction between the bacteria and particle components, is critical for the observed antimicrobial activity. [ 2 ] Encouraged by this observation, we proceeded to characterize the more advanced NPs (NH 2 -T-SNPs) and evaluated their antimicrobial activity and began to elucidate the biochemical mechanism of action of these antimicrobial NPs. show that for both bacteria, NH 2 -T-SNPs caused a reduction in growth in a dose-dependent manner, as did the T-SNPs, but with a reduced MBC, 5 μ g mL − 1 for both pathogens, instead of the former 10 μ g mL − 1 value for T-SNPs.…”

Section: Antimicrobial Activity Of Nh 2 -T-snpsmentioning

confidence: 94%

“…In our previous study, [ 2 ] we showed that T-SNPs posses superior antimicrobial activity compared to the free biocide (an increase of 5-6 log in killing capacity). In the current work, we continued to explore the aforementioned properties with second generation NPs that contained an outer polycationic shell containing polyNH 2 species.…”

Section: Nh 2 -T-snps' Synthesis and Characterizationmentioning

confidence: 98%

“…[ 9 ] The synthesis of NH 2 -T-SNPs was accomplished via a post-synthetic modifi cation of NPs containing covalently-bound triclosan (T-SNPs). [ 2 ] Briefl y, we used a modifi ed Stöber method to prepare core T-SNPs that were chemically grafted with (3-aminopropyl)triethoxysilane (APTES, EtOH, rt) as a grafting surface-modifying agent (Figure 1 ). The free silanol groups present on the surface of T-SNPs can undergo condensation with the triethoxy moiety of APTES to form new SiϪOϪSi bonds.…”

Section: Nh 2 -T-snps' Synthesis and Characterizationmentioning

confidence: 99%

“…The method allows for monodispersed silica NPs to be formed, as well as enabling the incorporation of various functionalities during the synthesis process. As stated in our previous study, [ 2 ] there are two main routes that can enable the introduction of a functional group or a guest molecule into the forming inorganic network, i.e., building on a non-covalent physical or a covalent linkage between both components. In our study we chose to covalently incorporate the known biocide, triclosan, into the silica network of SiO 2 NPs to afford the corresponding hybrid triclosan-bound NPs.…”

mentioning

confidence: 98%

“…[4][5][6][7] In our previous work, we have developed a linker molecule that enables covalent binding between the inorganic matrix of hybrid silica NPs and triclosan using a carbamate linkage. [ 2 ] This covalent carbamate bond within the silicate linker (blue part of TTESPC species, Figure 1 ) and its biologically-active component (red part of TTESPC species, Figure 1 ) have been specifi cally designed to be readily hydrolyzed by esterases on one hand but to withstand spontaneous hydrolysis (contacting medium) on the other hand ( Figure 1 and Scheme 1). Subsequently, the locally released active triclosan molecules act upon their ENR target within the bacteria cell itself.…”

mentioning

confidence: 99%

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Improved Triclosan Delivery by a Novel Silica‐Based Nanocomposite

Makarovsky¹,

Lellouche²,

Lellouche³

et al. 2012

Adv Healthcare Materials

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In this study, we report on the design, synthesis, and full characterization of a covalently-linked, triclosan silica-based nanoparticles (T-SNPs), coated with a polyaminated shell (NH2 -T-SNPs). Various techniques are used to elucidate and rationalize the potential biological mechanism of action of these novel nanoparticles. NH2 -T-SNPs are found to be potently bactericidal with no detectable lag time for the antimicrobial activity against E. coli and S. aureus. In this context, we also prove that triclosan is the chemical agent that mediated the bactericidal activity of these chemically-modified NPs. The obtained experimental data allows us to pinpoint the actual minimal bactericidal concentrations (MBCs) of triclosan-bound NPs by quantifying intracellular triclosan concentrations. Furthermore, we conduct preliminary cytotoxicity studies, which show that triclosan bound NPs are less cytotoxic (2000 fold) in vitro compared to free-triclosan when tested with various human and mammalian cell lines. Taken together, our results further support the characterization and development of these new nanoscale materials for various biomedical applications.

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Section: Antimicrobial Activity Of Nh 2 -T-snpsmentioning

confidence: 94%

Section: Nh 2 -T-snps' Synthesis and Characterizationmentioning

confidence: 98%

Section: Nh 2 -T-snps' Synthesis and Characterizationmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Improved Triclosan Delivery by a Novel Silica‐Based Nanocomposite

Makarovsky¹,

Lellouche²,

Lellouche³

et al. 2012

Adv Healthcare Materials

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Colloidal Gold Nanoclusters Spiked Silica Fillers in Mixed Matrix Coatings: Simultaneous Detection and Inhibition of Healthcare‐Associated Infections

Alsaiari

Hammami

Croissant

et al. 2017

Adv Healthcare Materials

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Healthcare-associated infections (HAIs) are the infections that patients get while receiving medical treatment in a medical facility with bacterial HAIs being the most common. Silver and gold nanoparticles (NPs) have been successfully employed as antibacterial motifs; however, NPs leaching in addition to poor dispersion and overall reproducibility are major hurdles to further product development. In this study, the authors design and fabricate a smart antibacterial mixed-matrix membrane coating comprising colloidal lysozyme-templated gold nanoclusters as nanofillers in poly(ethylene oxide)/poly(butylene terephthalate) amphiphilic polymer matrix. Mesoporous silica nanoparticles-lysozyme functionalized gold nanoclusters disperse homogenously within the polymer matrix with no phase separation and zero NPs leaching. This mixed-matrix coating can successfully sense and inhibit bacterial contamination via a controlled release mechanism that is only triggered by bacteria. The system is coated on a common radiographic dental imaging device (photostimulable phosphor plate) that is prone to oral bacteria contamination. Variation and eventually disappearance of the red fluorescence surface under UV light signals bacterial infection. Kanamycin, an antimicrobial agent, is controllably released to instantly inhibit bacterial growth. Interestingly, the quality of the images obtained with these coated surfaces is the same as uncoated surfaces and thus the safe application of such smart coatings can be expanded to include other medical devices without compromising their utility.

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Encapsulation of Biosynthesized Nanosilver in Silica Composites for Sustainable Antimicrobial Functionality

et al. 2018

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Silver nanoparticles (AgNPs) have become known as a broad‐spectrum antimicrobial agent. The antimicrobial activity of AgNPs is dependent on the particle size and the dispersion status. In this study, a simple and effective approach is developed for sequestering the biosynthesized AgNPs in silica composites during the gel formation of MCM‐41. Composites with different Ag concentrations of 0.034% (Ag1@MCM‐41), 0.151% (Ag2@MCM‐41), and 0.369% (Ag3@MCM‐41) are synthesized and then heated at 400 °C to produce Ag1@MCM‐41H, Ag2@MCM‐41H, and Ag3@MCM‐41H, respectively. The samples are characterized by flame atomic absorption spectrometry, Fourier‐transform infrared spectroscopy, X‐ray diffraction, N2 physisorption, scanning electron microscopy, transmission electron microscopy, and thermogravimetric analysis. The AgNPs are confirmed to be highly dispersed in the amorphous silica framework. The antimicrobial activity of the AgNP–silica samples is investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans using the cup–plate and the plate‐count techniques. The results show an excellent antimicrobial effect of these samples against the studied microorganisms. Importantly, the AgNP–silica samples are found to be stable up to 58 months under ambient conditions. These stable and powerful antimicrobial composites provide a more practical and effective strategy for combating biomedical pathogens and public health threats.

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Novel Triclosan-Bound Hybrid-Silica Nanoparticles and their Enhanced Antimicrobial Properties

Cited by 41 publications

References 56 publications

Improved Triclosan Delivery by a Novel Silica‐Based Nanocomposite

Improved Triclosan Delivery by a Novel Silica‐Based Nanocomposite

Colloidal Gold Nanoclusters Spiked Silica Fillers in Mixed Matrix Coatings: Simultaneous Detection and Inhibition of Healthcare‐Associated Infections

Encapsulation of Biosynthesized Nanosilver in Silica Composites for Sustainable Antimicrobial Functionality

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