Novel use of Kombucha consortium to reduce <scp><i>Escherichia coli</i></scp> in dairy shed effluent

Laurenson, Seth; Villamizar, Laura; Ritchie, Willis; Scott, Sonya M.; Mackay, Michael

doi:10.1002/jsfa.11250

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…Escherichia coli has the potential for antibiotic‐resistant acquisition and causes mortality and diseases in wildlife (Furtula et al, 2010). E. coli is output from dairy farms in runoff that comes into contact with animal feces deposited on paddocks (Laurenson et al, 2021). Another significant source of E. coli on dairy farms is the effluent that is produced during the washing down of milking plants, yards, and races.…”

Section: Discussionmentioning

confidence: 99%

High toxicity of agro‐industrial wastewater on aquatic fauna of a South American stream: Mortality of aquatic turtles and amphibian tadpoles as bioindicators of environmental health

Cuzziol Boccioni,

Peltzer,

Attademo

et al. 2024

Water Environment Research

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The aim of this study was to characterize an aquatic system of Santa Fe province (Argentina) receiving wastewater from agro‐industrial activities (mainly dairy) by in situ assessment (fauna mortality, physicochemical, microbiological, and pesticide residues measurement), and ecotoxicity bioassays on amphibian tadpoles. Water and sediment samples were obtained from the Los Troncos Stream (LTS), previous to the confluence with the “San Carlos” drainage channel (SCC), and from the SCC. Biological parameters (mortality and sublethal biomarkers) were used to evaluate ecotoxicity during 10‐day exposure of Rhinella arenarum tadpoles to LTS and SCC samples. Nine pesticides were detected in both LTS and SCC. Chemical and biochemical oxygen demand, ammonia, and coliform count recorded in SCC greatly exceeded limits for aquatic life protection. At SCC and LTS after the confluence with SCC, numerous dying and dead aquatic turtles (Phrynops hilarii) were recorded. In the ecotoxicity assessment, no mortality of tadpoles was observed in LTS treatment, whereas total mortality (100%) was observed in SCC treatments in dilution higher than 50% of water and sediment. For SCC, median lethal concentration and the 95% confidence limits was 18.30% (14.71–22.77) at 24 h; lowest‐observed and no‐observed effect concentrations were 12.5% and 6.25%, respectively. Oxidative stress and neurotoxicity were observed in tadpoles exposed to 25% SCC dilution treatment. In addition, there was a large genotoxic effect (micronuclei test) in all sublethal SCC dilution treatments (6.25%, 12.5%, and 25%). These results alert about the high environmental quality deterioration and high ecotoxicity for aquatic fauna of aquatic ecosystems affected by agro‐industrial wastewater.Practitioner points Great mortality of turtles was observed in a basin with a high load of agro‐industrial wastewater. San Carlos Channel (SCC), where effluents are spilled, is environmentally deteriorated. The water‐sediment matrix of SCC caused 100% lethality in tadpoles. SCC dilutions caused neurotoxicity, oxidative stress, and genotoxicity on tadpoles.

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Section: Discussionmentioning

confidence: 99%

High toxicity of agro‐industrial wastewater on aquatic fauna of a South American stream: Mortality of aquatic turtles and amphibian tadpoles as bioindicators of environmental health

Cuzziol Boccioni,

Peltzer,

Attademo

et al. 2024

Water Environment Research

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“…In a recent study, Laurenson et al [ 63 ] tested the effectiveness of Kombucha SCOBY (symbiotic culture of bacteria and yeast) in reducing the concentration of Escherichia coli in dairy effluent shed (SDR). In this study, the SCOBY kombucha was highly effective in reducing the number of E. coli colony forming units (CFU) to undetectable levels.…”

Section: Discussionmentioning

confidence: 99%

“…Kombucha was highly effective in reducing the number of Eschericha coli colony-forming units (CFU) to undetectable levels [63]. Pathogenic bacteria: Escherichia coli O157:H7, Shigella dysenteriae, Salmonella Typhi and Vibrio cholera were inhibited by kombucha [61].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Biological Significance of Probiotic Microorganisms from Kefir and Kombucha: A Review

da Anunciação,

Guedes,

Tavares

et al. 2024

Microorganisms

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(1) Background: The human microbiota is essential for maintaining a healthy body. The gut microbiota plays a protective role against pathogenic bacteria. Probiotics are live microorganisms capable of preventing and controlling gastrointestinal and balancing the immune system. They also aid in better nutrients and vitamins absorption. Examples of natural probiotic cultures are kefir and kombucha. (2) Methods: Therefore, the aim of this review was to address the beneficial properties of probiotic kefir and kombucha using a Boxplot analysis to search for scientific data in the online literature up to January 2024: (Latin American and Caribbean Health Sciences (LILACS), PubMed, Medical Literature Analysis (MED-LINE), Science Direct, Google Scholar/Google Academic, Bioline Inter-national and Springer Link). Boxplots showed the summary of a set of data “Index Terms—Keywords” on kefir and kombucha in three languages (English, Portuguese and Spanish). (3) Results: Google Scholar was the database with the highest number of articles found, when the search for the keywords used in the study (containing ~4 × 106–~4 million articles available). This was Followed by the Science Direct database, containing ~3 × 106–~3 million articles available, and the BVS databases—Biblioteca Virtual de Saúde (Virtual Health Library) e Lilacs, both containing a value of ~2 × 106–~2 million articles available. The databases containing the smallest number of articles found were Nutrients and Medline, both containing a value of ≤0.1 × 106–≤100 thousand articles. (4) Conclusions: Scientific studies indicate that kefir and kombucha certainly contain various functional properties, such as antimicrobial, antitumor, anticarcinogenic and immunomodulatory activity, in addition to having a microbiological composition of probiotic bacteria and yeasts. Kefir and kombucha represent key opportunities in the food and clinic/medical fields.

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“…4 Until recently, kombucha cellulose was considered a waste material from the fermentation process, but given these physical and chemical properties, this biomaterial has found application in a number of areas. These include pyrolytic generation of graphene oxide, 5 formation of hydrogel cellulose, 6 reduction of Escherichia coli in dairy shed effluent, 7 and fabrication of electrolyte membranes, 8 with all structural modifications of the cellulose being chemically driven. In the present study, we use mechanical energy to modify the physical properties of cellulose, thereby avoiding the need for chemical treatment which can generate a waste stream.…”

Section: ■ Introductionmentioning

confidence: 99%

Continuous Flow Vortex Fluidic Transformation of Kombucha Cellulose into More Compact and Crystalline Fibers

Jellicoe

Luo

et al. 2022

ACS Sustainable Chem. Eng.

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A chemical-free and scalable novel route for generating more compact and crystalline cellulose from cellulosic biofilms formed at the air−water interface as an otherwise waste byproduct from the kombucha tea beverage fermentation industry has been developed. This involves processing the byproduct by treatment with aqueous 1 M NaOH at 50 °C and then 1% glacial acetic acid followed by washing with distilled water until the pH of the washing water is 7, followed by continuous flow processing in the vortex fluidic device (VFD). This thin-film microfluidic platform houses a 20 mm outside diameter and 17.5 mm inside diameter quartz tube with a hemispherical base. The induced high shear mechanical energy arising from the topological fluid flows within the thin film when the tube is tilted at θ +45°and spun at ω 6k rpm converts the kombucha cellulose to smaller fiber diameters ∼70 nm cf. ∼127 nm preVFD processing. The material is characterized using dynamic light scattering, scanning electron microscopy, atomic force microscopy, X-ray diffraction, thermogravimetric analysis/differential scanning calorimetry, Fourier transform infrared, and Brunauer−Emmett−Teller. The modification of the cellulose is understood mechanistically by the high shear topological fluid flow from the Coriolis force from the base of the tube, forcing the individual cellulose polymer strands together in an ordered array. Such transient localized high shear flow associated with high temperatures and pressures over the fibers on the surface of the tube force the backbone long cellulose strands together resulting in increased crystallinity. The findings lay the foundation for transforming a byproduct from kombucha tea beverage fermentation into unique material for other applications.

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Novel use of Kombucha consortium to reduce Escherichia coli in dairy shed effluent

Cited by 6 publications

References 21 publications

High toxicity of agro‐industrial wastewater on aquatic fauna of a South American stream: Mortality of aquatic turtles and amphibian tadpoles as bioindicators of environmental health

High toxicity of agro‐industrial wastewater on aquatic fauna of a South American stream: Mortality of aquatic turtles and amphibian tadpoles as bioindicators of environmental health

Biological Significance of Probiotic Microorganisms from Kefir and Kombucha: A Review

Continuous Flow Vortex Fluidic Transformation of Kombucha Cellulose into More Compact and Crystalline Fibers

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