Novel x-Type High-Molecular-Weight Glutenin Genes From <i>Aegilops tauschii</i> and Their Implications on the Wheat Origin and Evolution Mechanism of <i>Glu-D1-1</i> Proteins

Zhang, Yanzhen; Li, Xiaohui; Wang, Aili; An, Xianghai; Zhang, Qian; Pei, Yuhe; Gao, Liyan; Ma, Wujun; Appels, R.; Yan, Yueming

doi:10.1534/genetics.107.077412

Cited by 65 publications

(46 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The wide spread of spontaneous amphidiploidization in the T. turgidum-Ae. tauschii F 1 hybrids found in our study, therefore, supports the multi-origin hypothesis for the hexaploid wheat proposed by many wheat scientists (Dvorak et al 1998;Talbert et al 1998;Lelley et al 2000;Caldwell et al 2004;Giles and Brown 2006;Zhang et al 2008b).…”

Section: Discussionsupporting

confidence: 92%

Frequent occurrence of unreduced gametes in Triticum turgidum–Aegilops tauschii hybrids

et al. 2009

View full text Add to dashboard Cite

Spontaneous chromosome doubling via union of unreduced (2n) gametes has been thought to be the way that common wheat (Triticum aestivum L.) was originated from the hybridization of T. turgidum L. with Ae. tauschii Cosson. Previous works have observed unreduced gametes in F 1 hybrids of Ae. tauschii with six of the eight T. turgidum subspecies. It is not clear, however, whether the formation of these unreduced gametes is a norm in the F 1 hybrids. In the present study, we tried to answer this question by assessing the occurrence frequency of unreduced gametes in 115 T. turgidum-Ae. tauschii hybrid combinations, involving 76 genotypes of seven T. turgdium subspecies and 24 Ae. tauschii accessions. Our data show that these hybrid combinations differed significantly (P B 0.01, F = 11.40) in selfed seedset, an indicator for production of unreduced gametes. This study clearly showed that meiotic restitution genes are widely distributed within T. turgidum. However, significant differences were found between as well as within T. turgidum subspecies and in the interaction of the T. turgidum genotypes with those of Ae. taushii. The possible application of the meiotic restitution genes from T. turgidum in production of double haploids is also discussed.

show abstract

Section: Discussionsupporting

confidence: 92%

Frequent occurrence of unreduced gametes in Triticum turgidum–Aegilops tauschii hybrids

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The recent findings of a chimeric HMW-GS gene from the S t genome of P. stipifolia (Z. ) and a large fragment deletion in a HMW glutenin subunit gene resulting from illegitimate recombination through bacterial expression (Zhang et al 2008) strongly support this hypothesis. Considering that different direct repeats are commonly present in storage protein genes, and that the i-and m-types of Red and blank frame represented LMW-i-and LMW-mtype genes, respectively.…”

Section: Pcr Amplification and Cloning Of Lmw-gs Genesmentioning

confidence: 91%

“…However, recent studies have shown that illegitimate recombination may be an important genetic mechanism driving genome expansion and contraction and modular protein evolution throughout the tree of life (Patthy 1999;Devos et al 2002;Katju and Lynch 2003). More recently, an X-linked testes chimeric gene by illegitimate recombination in Drosophila has been identified (Arguello et al 2006), and illegitimate recombination can result in a large fragment deletion in Glu-D-1-1 genes and generation of new alleles (Zhang et al 2008). In wheat, eight chimeric genes resulting from g-gliadins and LMW-GS genes were found, which supposedly resulted from crossing over between the Gli-1 and Glu-3 loci (Nagy et al 2005).…”

Section: Pcr Amplification and Cloning Of Lmw-gs Genesmentioning

confidence: 99%

A Novel Chimeric Low-Molecular-Weight Glutenin Subunit Gene From the Wild Relatives of Wheat Aegilops kotschyi and Ae. juvenalis: Evolution at the Glu-3 Loci

Gao

et al. 2008

Genetics

Self Cite

View full text Add to dashboard Cite

Four LMW-m and one novel chimeric (between LMW-i and LMW-m types) low-molecular-weight glutenin subunit (LMW-GS) genes from Aegilops neglecta (UUMM), Ae. kotschyi (UUSS), and Ae. juvenalis (DDMMUU) were isolated and characterized. Sequence structures showed that the 4 LMW-m-type genes, assigned to the M genome of Ae. neglecta, displayed a high homology with those from hexaploid common wheat. The novel chimeric gene, designed as AjkLMW-i, was isolated from both Ae. kotschyi and Ae. juvenalis and shown to be located on the U genome. Phylogentic analysis demonstrated that it had higher identity to the LMW-m-type than the LMW-i-type genes. A total of 20 single nucleotide polymorphisms (SNPs) were detected among the 4 LMW-m genes, with 13 of these being nonsynonymous SNPs that resulted in amino acid substitutions in the deduced mature proteins. Phylogenetic analysis demonstrated that it had higher identity to the LMW-m-type than the LMW-i-type genes. The divergence time estimation showed that the M and D genomes were closely related and diverged at 5.42 million years ago (MYA) while the differentiation between the U and A genomes was 6.82 MYA. We propose that, in addition to homologous recombination, an illegitimate recombination event on the U genome may have occurred 6.38 MYA and resulted in the generation of the chimeric gene AjkLMW-i, which may be an important genetic mechanism for the origin and evolution of LMW-GS Glu-3 alleles as well as other prolamin genes.

show abstract

“…Another reason might be immunochemical differences between 1Dx5 and 1Dx2 in common wheats and 1Dx5 t and 1Dx2 t in Aegilops tauschii. It was found that there existed some differences between 1Dx5 and 1Dx5 t as well as 1Dx2 and 1Dx2 t in relative mobilities and gene structures (Yan et al 2003b;Zhang et al 2008). It was possible that greater immunochemical differences were present between 1Dx5 t and 1Dx2 t in Aegilops tauschii and, therefore, they could be discriminated by mAb14587.…”

Section: Discrimination Of Allelic Pairs Of Hmw-gsmentioning

confidence: 99%

Characterization of monoclonal antibodies specific to wheat glutenin subunits and their correlation with quality parameters

Li¹,

Ji²,

Zhang³

et al. 2009

Can. J. Plant Sci.

Self Cite

View full text Add to dashboard Cite

2009. Characterization of monoclonal antibodies specific to wheat glutenin subunits and their correlation with quality parameters. Can. J. Plant Sci. 89: 11Á19. Immunochemical methods are very useful in predicting the quality of wheat and differentiating alleles. In order to prepare appropriate monoclonal antibodies, HMW-GS 1Bx13 and 1By16 from spelt wheat were used as antigens to immunize BALB/C mice. Four monoclonal antibodies (mAbs) were obtained and designated 24231, 24245, 14588 and 14587, respectively. Results of Western blot showed that mAbs 24231 and 24245 prepared against 1Bx13 bound only to LMW-GS. The mAb 14588 prepared against 1By16 bound strongly to LMW-GS, but weakly to 1By and some 1Dy type HMW-GS. The mAb 14587 prepared against 1By16 bound only to 1Dx HMW-GS. The results of indirect ELISA and statistical analysis showed that correlations between mAb 24231 and development time and stability were significantly (P B0.05) and highly significantly (PB0.01) negative, respectively, whereas those of mAb 24245 with development time and extensibility were highly significantly (PB0.01) and significantly (PB0.05) negative, respectively. Significantly (PB0.05) and highly significantly (PB0.01) positive correlations were observed between mAb 14588 and stability and development time. Mean differential binding of mAb 14587 with 1Dx5 t and 1Dx2 t subunits from Aegilops tauschii was highly significant (PB0.01), suggesting that ELISA could potentially be used as an effective screening tool during direct genetic transfer of desirable glutenin subunits from Aegilops tauschii to hexaploid wheat.. 2009. Caracte´risationCaracte´risation des anticorps monoclonaux spe´cifiquesspe´cifiques aux sous-unite´sunite´s de la glute´nineglute´nine du bleét correíation avec lesparamè tres de la qualite´.qualite´. Can. J. Plant Sci. 89: 11Á19. L'immunochimie s'est ave´reéave´reéparticuliè rement utile pour pre´direpre´dire la qualite´duqualite´du bleét la diffe´renciationdiffe´renciation desallè les. Pour pre´parerpre´parer les anticorps monoclonaux ne´cessairesne´cessaires, les auteurs ont pris les sous-unite´sunite´s de la glute´nineglute´nine a` haut poids mole´culairemole´culaire 1Bx13 et 1By16 de l'e´peautree´peautre commeantigè nes et s'en sont servi pour immuniser des souris BALB/C. Ils ont ainsi obtenu quatre anticorps monoclonaux (mAb) qu'ils ont baptise´sbaptise´s respectivement 24231, 24245, 14588 et 14587. Les re´sultatsre´sultats de la me´thodeme´thode de Western indiquent que les mAb 24231 et 24245 pre´pare´spre´pare´pre´pare´s avec 1Bx13 ne se lient qu'aux sous-unite´sunite´s de la glute´nineglute´nine a` faible poids mole´culairemole´culaire. Le mAb 14588 pre´pareávecpre´pareávec 1By16 se lie fortement aux sous-unite´sunite´s de la glute´nineglute´nine a` faible poids mole´culairemole´culaire, mais se lie peu aux sous-unite´sunite´s a` haut poids mole´culairemole´culaire 1By et a` certaines du type 1Dy. Le mAb 14587 pre´pareávecpre´pareávec 1By16 ne se lie qu'aux sous-unite´sunite´s de la glute´nineglute´nine a` haut...

show abstract

Novel x-Type High-Molecular-Weight Glutenin Genes From Aegilops tauschii and Their Implications on the Wheat Origin and Evolution Mechanism of Glu-D1-1 Proteins

Cited by 65 publications

References 52 publications

Frequent occurrence of unreduced gametes in Triticum turgidum–Aegilops tauschii hybrids

Frequent occurrence of unreduced gametes in Triticum turgidum–Aegilops tauschii hybrids

A Novel Chimeric Low-Molecular-Weight Glutenin Subunit Gene From the Wild Relatives of Wheat Aegilops kotschyi and Ae. juvenalis: Evolution at the Glu-3 Loci

Characterization of monoclonal antibodies specific to wheat glutenin subunits and their correlation with quality parameters

Contact Info

Product

Resources

About