Novel Zeff imaging method for deep internal areas using back-scattered X-rays

Yoneyama, Akio; Kawamoto, Masahide; Baba, Rika

doi:10.1038/s41598-019-54907-3

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…Achieving both high spectral and angular resolution over large solid angles, such as with a pnCCD detector 30 would enable additional maps of the sample structure to be obtained from the Compton and elastic scattering. For example, the ratio of elastic to inelastic scattering could be used to map the effective atomic number 31 , and diffraction tomography data 32 could be simultaneously measured by discriminating photons based on their energy.…”

Section: Discussionmentioning

confidence: 99%

Dose-efficient scanning Compton X-ray microscopy

Dresselhaus

Ivanov

et al. 2023

Light Sci Appl

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The highest resolution of images of soft matter and biological materials is ultimately limited by modification of the structure, induced by the necessarily high energy of short-wavelength radiation. Imaging the inelastically scattered X-rays at a photon energy of 60 keV (0.02 nm wavelength) offers greater signal per energy transferred to the sample than coherent-scattering techniques such as phase-contrast microscopy and projection holography. We present images of dried, unstained, and unfixed biological objects obtained by scanning Compton X-ray microscopy, at a resolution of about 70 nm. This microscope was realised using novel wedged multilayer Laue lenses that were fabricated to sub-ångström precision, a new wavefront measurement scheme for hard X rays, and efficient pixel-array detectors. The doses required to form these images were as little as 0.02% of the tolerable dose and 0.05% of that needed for phase-contrast imaging at similar resolution using 17 keV photon energy. The images obtained provide a quantitative map of the projected mass density in the sample, as confirmed by imaging a silicon wedge. Based on these results, we find that it should be possible to obtain radiation damage-free images of biological samples at a resolution below 10 nm.

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Section: Discussionmentioning

confidence: 99%

Dose-efficient scanning Compton X-ray microscopy

Dresselhaus

Ivanov

et al. 2023

Light Sci Appl

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show abstract

“…Achieving both high spectral and angular resolution over large solid angles, such as with a pnCCD detector 29 would enable additional maps of the sample structure to be obtained from the Compton and elastic scattering. For example, the ratio of elastic to inelastic scattering could be used to map the effective atomic number 30 , and diffraction tomography data 31 could be simultaneously measured by discriminating photons based on their energy.…”

Section: Discussionmentioning

confidence: 99%

Dose-efficient Scanning Compton X-ray Microscopy

Bajt¹,

Li²,

Dresselhaus³

et al. 2023

Preprint

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The highest resolution of images of soft matter and biological materials is ultimately limited by modification of the structure, induced by the necessarily high energy of short-wavelength radiation. Imaging the inelastically scattered X-rays at a photon energy of 60 keV (0.02 nm wavelength) offers greater signal per energy transferred to the sample than coherent-scattering techniques such as phase-contrast microscopy and projection holography. We present images of dried, unstained, and unfixed biological objects obtained by scanning Compton X-ray microscopy, at a resolution of about 40 nm. This microscope was realised using novel wedged multilayer Laue lenses that were fabricated to sub-ångström precision, a new wavefront measurement scheme for hard X rays, and efficient pixel-array detectors. The doses required to form these images were as little as 0.02% of the tolerable dose and 0.05% of that needed for phase-contrast imaging at similar resolution using 12 keV photon energy. The images obtained provide a quantitative map of the projected mass density in the sample, as confirmed by imaging a silicon wedge. Based on these results, we find that it should be possible to obtain radiation damage-free images of biological samples at a resolution below 10 nm.

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Monte Carlo simulation of the mass attenuation coefficient and effective atomic number of the Eremurus-Rhizophora ssp. particleboard phantom at the mammography energy range

Tousi

2022

Progress in Nuclear Energy

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Novel Zeff imaging method for deep internal areas using back-scattered X-rays

Cited by 6 publications

References 20 publications

Dose-efficient scanning Compton X-ray microscopy

Dose-efficient scanning Compton X-ray microscopy

Dose-efficient Scanning Compton X-ray Microscopy

Monte Carlo simulation of the mass attenuation coefficient and effective atomic number of the Eremurus-Rhizophora ssp. particleboard phantom at the mammography energy range

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