NOVEOS and ImmunoCAP Have Similar Performances for Diagnosing Food Allergies

Trouche-Estival, Benjamin; Vitte, Joana; Martin-Blondel, Audrey; Michelet, Marine; Gruzelle, Vianney; Didier, Alain; Guilleminault, Laurent; Mailhol, Claire; Rivera, Silvia Martinez; De Lima Correia, Anaïs; Taurus, Camille; Blancher, Antoine; Goret, Julien; Klingebiel, Caroline; Apoil, Pol André

doi:10.1016/j.jaip.2024.02.037

The Journal of Allergy and Clinical Immunology: In Practice

2024

DOI: 10.1016/j.jaip.2024.02.037

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NOVEOS and ImmunoCAP Have Similar Performances for Diagnosing Food Allergies

Benjamin Trouche-Estival,

Joana Vitte,

Audrey Martin-Blondel

et al.

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Cited by 3 publications

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Detection of egg white allergy in children by specific IgE microarray chemiluminescence immunoassay

Zhang,

Yuan,

Yang

et al. 2025

Clinica Chimica Acta

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Detection of egg white allergy in children by specific IgE microarray chemiluminescence immunoassay

Zhang,

Yuan,

Yang

et al. 2025

Clinica Chimica Acta

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LuLIPLEX: A Fast, Highly Sensitive, and Multiplexed Method for the Detection of IgE Against Major Allergens

Leveque,

Pecalvel,

Casanovas

et al. 2024

Allergy

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BackgroundDiagnosis of allergies is mostly based on the patient's clinical history and allergen provocation tests. Determination of specific IgE (sIgE) profiles can be performed to support allergy diagnosis. This is commonly done in vivo by the skin prick test or in vitro with automated systems. Several platforms exist to quantify sIgE levels, but all these methods require access to specific instruments, often delaying the test's results. The IgE luciferase‐linked immunosorbent assay (LuLISA) allows bioluminescent quantification of IgE against peanut in microliter samples, but this method awaits extension to other allergens. This study aimed to validate a new method, named LuLIPLEX, for multiplexed bioluminescent detection of sIgE against 20 major molecular allergens.MethodsQuantification of sIgE against 12 recombinant or purified food allergens and eight aeroallergens was performed by LuLIPLEX versus standard IgE detection methods (ImmunoCAP, ISAC, ALEX, or NOVEOS). Multiplexed detection of IgE against these 20 allergens was performed within 45 min using 50 μL of serum, plasma, or whole blood samples.ResultsA head‐to‐head comparison between LuLIPLEX and standard IgE detection methods showed a high correlation among all allergens tested. sIgE profiles in polysensitized subjects could be determined within 45 min in serum and plasma samples, as well as using a single drop of capillary blood.ConclusionsLuLIPLEX is a rapid and sensitive method to quantify sIgE levels against multiple allergens. Given that the test is very fast and can be performed on small and inexpensive luminometers, the IgE LuLIPLEX could allow point‐of‐care testing of sIgE profiles in allergic subjects.

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Molecular allergology: a clinical laboratory tool for precision diagnosis, stratification and follow-up of allergic patients

Giusti,

Guemari,

Perotin

et al. 2024

Clinical Chemistry and Laboratory Medicine (CCLM)

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Identification of the molecular culprits of allergic reactions leveraged molecular allergology applications in clinical laboratory medicine. Molecular allergology shifted the focus from complex, heterogeneous allergenic extracts, e.g. pollen, food, or insect venom, towards genetically and immunologically defined proteins available for in vitro diagnosis. Molecular allergology is a precision medicine approach for the diagnosis, stratification, therapeutic management, follow-up and prognostic evaluation of patients within a large range of allergic diseases. Exclusively available for in vitro diagnosis, molecular allergology is nonredundant with any of the current clinical tools for allergy investigation. As an example of a major application, discrimination of genuine sensitization from allergen cross-reactivity at the molecular level allows the proper targeting of the culprit allergen and thus dramatically improves patient management. This review aims at introducing clinical laboratory specialists to molecular allergology, from the biochemical and genetic bases, through immunological concepts, to daily use in the diagnosis and management of allergic diseases.

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NOVEOS and ImmunoCAP Have Similar Performances for Diagnosing Food Allergies

Cited by 3 publications

References 36 publications

Detection of egg white allergy in children by specific IgE microarray chemiluminescence immunoassay

Detection of egg white allergy in children by specific IgE microarray chemiluminescence immunoassay

LuLIPLEX: A Fast, Highly Sensitive, and Multiplexed Method for the Detection of IgE Against Major Allergens

Molecular allergology: a clinical laboratory tool for precision diagnosis, stratification and follow-up of allergic patients

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