Noviherbaspirillum pedocola sp. nov., isolated from oil-contaminated experimental soil

Abstract

An orange-coloured, rod-shaped, and aerobic bacterial strain DKR-6 T was isolated from oil-contaminated experimental soil. The strain was Gram-stain-negative, catalase and oxidase positive, and grew at temperature 10-42 °C, at pH 5.5-9.5, and at 0-3.0% (w/v) NaCl concentration. The phylogenetic analysis and 16S rRNA gene sequence analysis suggested that the strain DKR-6 T was affiliated to the genus Noviherbaspirillum, with the closest species being Noviherbaspirillum massiliense JC206 T (96.3% sequence similarity). The chemotaxonomic profiles revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine as the principal polar lipids; C 16:0 , C 17:0 cyclo, summed feature 3 (C 16:1 ω7c and/or C 16: 1 ω6c), and summed feature 8 (C 18:1 ω7c/or C 18:1 ω6c) as the main fatty acids; and Q-8 as a sole ubiquinone. The DNA G + C content was 61.6%. The polyphasic taxonomic features illustrated in this study clearly implied that strain DKR-6 T represents a novel species in the genus Noviherbaspirillum, for which the name Noviherbaspirillum pedocola sp. nov. is proposed with the type strain DKR-6 T (= KACC 22074 T = NBRC 114727 T ).

Bacteria degrading both n-alkanes and aromatic hydrocarbons are prevalent in soils

“…Furthermore, we indicated that bacteria so far not recognized as degraders may also participate in alkane conversion; namely the Noviherbaspirillum and Flavihumibacter taxa. Although there are no reports of alkane transformation by these bacteria, the representative of the former genus was previously isolated from oil-contaminated soil (Chaudhary et al 2021 ). Searching for alkB genes in the genomes belonging to these genera revealed the presence of 4 and 3 mentioned genes in respectively 14 genomes of Noviherbaspirillum and 11 genomes of Flavihumibacter (NCBI GenBank data from 31 May 2023, the result of sequence homology analysis performed using Blast).…”

Cellulomonas fulva sp. nov., isolated from oil-contaminated soil

“…Strain DKR-3 T was recovered from laboratory-prepared experimental oil-contaminated soil during a study of bioremediation and subjected to a polyphasic study for determination of its taxonomic position. The isolation, maintenance and preservation of strain DKR-3 T were performed following the methodology described in a previous study [10].…”

Noviherbaspirillum album sp. nov., an airborne bacteria isolated from an urban area of Beijing, China

“…Cells of this genus are Gram-stain-negative and non-spore-forming. The predominant quinone is Q-8, with major fatty acids being summed feature 3 (C 16 : 1 ω6c/C 16 : 1 ω7c), summed feature 8 (C 18 : 1 ω7c/C 18 : 1 ω6c), and straight-chain saturated fatty acids [1][2][3][4][5][6][7][8][9]. The polar lipid profile includes diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC) [1].…”

“…Flagellar-related proteins, including chemotaxis proteins, transcriptional activators, motor switch proteins, and biosynthesis proteins, were found in all analysed genomes (Table S3). This suggested that flagellar motility is common in the genus Noviherbaspirillum, although N. pedocola DKR-6 T has been reported to lack flagella [2]. *Summed features are fatty acids that cannot be resolved reliably from another fatty acid using the chromatographic conditions chosen.…”

“…Nestled within the family Oxalobacteraceae of the phylum Pseudomonadota, the genus Noviherbaspirillum comprises 15 species with validly published names. These species primarily originated from soil [1][2][3][4][5][6][7], with occasional findings in air [8,9] and human faecal flora [10]. Cells of this genus are Gram-stain-negative and non-spore-forming.…”