Novosphingobium endophyticum sp. nov. isolated from roots of Glycyrrhiza uralensis

Abstract

A novel Gram-staining-negative, non-motile, rod-shaped and aerobic bacterial strain, designated EGI 60015(T), was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, Northwest China. The 16S rRNA gene sequence of strain EGI 60015(T) was found to show 97.6% sequence similarity with Novosphingobium pentaromativorans US6-1(T). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clade with N. pentaromativorans US6-1(T) in the neighbor-joining tree. Q-10 was identified as the respiratory quinone of strain EGI 60015(T). The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c; 55.04%), summed feature 4 (C17:1 anteiso B and/or iso I; 18.34%) and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 8.53%). The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipids. The DNA G+C content of strain EGI 60015(T) was determined to be 66.6 mol%. The genomic DNA relatedness value between EGI 60015(T) and N. pentaromativorans US6-1(T) (54%) was below the 70% limit for species identification. Based on the result of the molecular studies supported by its morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60015(T) was considered to represent a novel species within the genus Novosphingobium, for which the name Novosphingobium endophyticum sp. nov. is proposed. The type strain is EGI 60015(T) (=CGMCC 1.15095(T) = KCTC 42486(T) = DSM 29948(T)).

Ochrobactrum teleogrylli sp. nov., a pesticide-degrading bacterium isolated from the insect Teleogryllus occipitalis living in deserted cropland

“…[1]. At the time of writing, the genus Ochrobactrum comprised 17 species with validly published names, including the recently described species Ochrobactrum endophyticum [2]. Type strains of species of the genus Ochrobactrum have been recovered from a diverse range of sources, including soil [3], plants and their rhizospheres [4–7], industrial environments [6, 8, 9], animals [10, 11], and humans [1, 12–14].…”

Novosphingobium aureum sp. nov., a marine bacterium isolated from salt flat sediment

“…Detailed fatty acid compositions of strain YJ-S2-02 T and the related strains are shown in Table S2. According to Table S2, the fatty acid profile of strain YJ-S2-02 T was similar to those of the eight reference strains and also other Novosphingobium species [4, 28, 31, 32], indicating that strain YJ-S2-02 T belongs to the genus Novosphingobium . Although some fatty acid constituents of strain YJ-S2-02 T differed in certain proportions from some references, the largest proportion was same in all reference strains as summed feature 8 (C 18 : 1 ω 6 c /ω7 c ).…”

“… *Data from: a , Jun et al [31]; b , Lee et al [5]; c , Suzuki et al [14]; d , Li et al [28]; e , Sheu et al [30]; f , Chaudhar et al [29]; g , Gupta et al [15]; h , Sohn et al [32]. …”

“…S3). The possession of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as the major polar lipids are common characteristics of the genus Novosphingobium [4] and also closely related type strains of YJ-S2-02 T [5,15,[28][29][30]. The major components of the cellular fatty acids (>10%) of strain YJ-S2-02 T were summed feature 8 ( *Data from: a, Jun et al [31]; b, Lee et al [5]; c, Suzuki et al [14]; d, Li et al [28]; e, Sheu et al [30]; f, Chaudhar et al [29]; g, Gupta et al [15]; h, Sohn et al [32].…”

Novosphingobium kaempferiae sp. nov., a phosphate-solubilizing bacterium isolated from stem of Kaempferia marginata Carey

“…Members of genus Novosphingobium are characterized as Gram-negative, rod-shaped, non-spore-forming and aerobic bacteria. Species of the genus Novosphingobium have been isolated from various habitats, including soil, seawater, sediment, activated sludge, oil-contaminated soil and hexachlorocyclohexane-contaminated soil [3][4][5][6][7], while several species of the genus have been isolated from rhizosphere and plants including Novosphingobium rhizosphaerae, Novosphingobium pokkalii, Novosphingobium arabidopsis, Novosphingobium oryzae, Novosphingobium gossypii, Novosphingobium endophyticum and Novosphingobium fluoreni [8][9][10][11][12][13][14]. During a study of microbial diversity and plant growth-promoting endophytic bacteria in Thailand, strain Sx8-5 T was isolated from stem of peacock ginger (Kaempferia marginata Carey) and characterized using polyphasic analysis, including phenotypic characteristics, ubiquinone, cellular fatty acid, polar lipid and genomic comparisons.…”