Novosphingobium taihuense sp. nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China

Liu, Zhi-Pei; Wang, Baojun; Liu, Ying-Hao; Liu, Shuang‐Jiang

doi:10.1099/ijs.0.63468-0

Cited by 136 publications

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References 28 publications

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“…The genus Novosphingobium currently comprises 14 recognized species. Members of the genus Novosphingobium are rather heterogeneous and originate from a variety of habitats including soil, coastal or freshwater sediments (Balkwill et al, 1997;Sohn et al, 2004;Liu et al, 2005;Suzuki & Hiraishi 2007), activated sludge/wastewater treatment plants (Neef et al, 1999;Fujii et al, 2003) and a contaminated groundwater bioremediation reactor (Tiirola et al, 2002). In 16S rRNA gene clone libraries of subsurface water samples of the acidic and humic acid-rich Lake Grosse Fuchskuhle (MecklenburgBrandenburg lake district, north-eastern Germany), a 16S rRNA gene sequence frequently appeared that was affiliated to the genus Novosphingobium (Glöckner et al, 2000;Burkert et al, 2003;Allgaier & Grossart, 2006).…”

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“…Strain FSW06-204d T exhibited a quinone system consisting of ubiquinone Q-10 (91 %) and Q-9 (9 %), which was in accordance with the characteristics of the genus (Busse et al, 1999;Takeuchi et al, 2001;Kämpfer et al, 2002;Tiirola et al, 2005). The polar lipid profile consisted of the major compounds phosphatidyldimethylethanolamine and phosphatidylethanolamine, moderate amounts of a highly hydrophobic yellow pigment stainable with a-naphthol, moderate amounts of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylglycerol, sphingoglycolipid, an unknown highly hydrophilic aminophosphoglycolipid, two unknown polar lipids and minor to trace amounts of one unknown glycolipid, two unknown phospholipids, two unknown polar lipids and another highly hydrophobic yellow pigment (see Supplementary Kämpfer et al (2002), for strains 4, 5, and 10 from Tiirola et al (2005), for strain 13 from Liu et al (2005) and for strain 14 from Suzuki & Hiraishi (2007). 2, Negative; +, positive; (+), weakly positive; pNA, para-nitroanilide; pNP, para-nitrophenyl; ND, not detected or not described.…”

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“…dSubstrate assimilation tests were performed with methods described by Liu et al (2005) and Suzuki & Hiraishi (2007). Fig.…”

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Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle

Glaeser

Kämpfer

Busse

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A yellow-to orange-pigmented, Gram-negative, rod-shaped, motile and non-spore-forming bacterium, strain FSW06-204d T , was isolated from subsurface water of the acidic bog lake, Lake Grosse Fuchskuhle (Brandenburg, Germany). Optimum growth of this strain occurred over a pH range from 5.5 to 6.0 and the growth rate strongly decreased at pH values above 6.5. In addition, the strain exhibited a low tolerance towards NaCl and grew only at a NaCl concentration of up to 0.5 %. 16S rRNA gene sequence analysis of strain FSW06-204d T showed the highest sequence similarity to Novosphingobium hassiacum W-51 T (96.7 %) and formed a distinct cluster with Novosphingobium nitrogenifigens DSM 19370 T (96.4 %) within the genus Novosphingobium. Strain FSW06-204d T shared a 21 bp signature gap with the latter species, a feature that is absent in all other members of the family Sphingomonadaceae. DNA-DNA hybridization of strain FSW06-204d T and N. nitrogenifigens DSM 19370 T showed a low relatedness value of 24 % (reciprocal: 39 %). The major respiratory quinone was ubiquinone Q-10 (91 %) and the predominant fatty acid was C 18 : 1 v7c (43.3 %). Two characteristic 2-hydroxy fatty acids, C 14 : 0 2-OH (8.1 %) and C 15 : 0 2-OH (6.5 %), were abundant. Polar lipids consisted mainly of phosphatidyldimethylethanolamine and phosphatidylethanolamine; however, only moderate amounts of sphingoglycolipids were present and phosphatidylcholine was lacking. Characterization by 16S rRNA gene sequence, physiological features, pigment analysis and polyamine, ubiquinone, polar lipid and fatty acid contents revealed that strain FSW06-204d T represents a novel species of the genus Novosphingobium within the class Alphaproteobacteria. The name Novosphingobium acidiphilum sp. nov. is proposed for this acidophilic and saltsensitive species with the type strain FSW06-204d T (5DSM 19966 T 5CCM 7496 T 5CCUG 55538 T ).

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Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle

Glaeser

Kämpfer

Busse

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…Differential characteristics between strain SLH-16 T and the type strains of related Novosphingobium species Strains: 1, SLH-16 T ; 2, N. taihuense JCM 12465 T ; 3, N. subterraneum DSM 12447 T ; 4, N. hassiacum DSM 14552 T ; 5, N. lentum DSM 13663 T . All data are from this study except the DNA G+C content of N. taihuense JCM 12465 T (Liu et al, 2005), N. subterraneum DSM 12447 T (Balkwill et al, 1997) and N. lentum DSM 13663 T (Tiirola et al, 2005).+, Positive reaction; (, negative reaction; ND, not determined. All strains grow well under aerobic conditions on R2A agar and nutrient agar at 30 8C.…”

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Novosphingobium piscinae sp. nov., isolated from a fish culture pond

Sheu

Chen

2016

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain designated SLH-16 T was isolated from a fish culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain SLH-16 T were Gram-stain-negative, aerobic, motile rods that were covered by large capsules and formed yellow colonies. Growth occurred at 20-40 8C (optimum, 37-40 8C), at pH 4.0-9.0 (optimum, pH 5.0-6.0) and with 0-0.5 % (w/v) NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SLH-16 T belonged to the genus Novosphingobium and was related most closely to Novosphingobium taihuense T3-B9 T with sequence similarity of 97.3 %. The major fatty acids (.10 %) of strain SLH-16 T were C 18 : 1 v7c, summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) and C 16 : 0 .The major 2-hydroxy fatty acid was C 14 : 0 2-OH. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine and several uncharacterized lipids. The major polyamine was spermidine. The major isoprenoid quinone was Q-10.The DNA G+C content was 65.2 mol%. The DNA-DNA hybridization value for strain SLH-16 T and the type strain of N. taihuense was less than 43.2 %. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain SLH-16 T represents a novel species in the genus Novosphingobium, for which the name Novosphingobium piscinae sp. nov. is proposed. The type strain is SLH-16 T (5BCRC 80888 T 5LMG 28418 T 5KCTC 42194 T ).The genus Novosphingobium in the family Sphingomonadaceae of the class Alphaproteobacteria was proposed by Takeuchi et al. (2001). At the time of writing, this genus comprises 34 recognized species (http://www.bacterio.net/ novosphingobium.html). Members of the genus Novosphingobium are characterized as Gram-stain-negative, nonspore-forming, rod-shaped, non-motile or motile, aerobic and chemo-organotrophic bacteria; chemotaxonomically they possess C 18 : 1 v7c as the predominant fatty acid and C 14 : 0 2-OH as the major 2-hydroxy fatty acid, with spermidine as the major polyamine, Q-10 as the major ubiquinone, glycosphingolipids and a DNA G+C content of between 62 and 71 mol% (Takeuchi et al., 2001;Lin et al., 2014; Kämpfer et al., 2015). The present study was carried out to clarify the taxonomic position of a novel species of the genus Novosphingobium by using a polyphasic taxonomic approach.During our investigations on the biodiversity of bacteria in the water of a fish culture pond in the Jiali District (GPS location: 238 109 380 N 1208 109 510 E), Tainan County, Taiwan, a novel yellow-pigmented bacterium, designated SLH-16 T , was isolated and selected for detailed taxonomic analyses. Strain SLH-16 T was maintained on R2A agar (BD Difco) at 25 8C, and preserved at (80 8C as a suspension in 20 % (v/v) glycerol R2A broth or by lyophilization w...

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“…The nomenclatural system was replaced by the proposal of Takeuchi et al (2001) and has been generally accepted and used (Kämpfer et al, 2002;Fujii et al, 2003;Sohn et al, 2004;Liu et al, 2005;Tiirola et al, 2005;Addison et al, 2007;Lim et al, 2007;Suzuki & Hiraishi, 2007;Glaeser et al, 2009;Gupta et al, 2009;Yuan et al, 2009). At the time of this writing, the genus Novosphingobium comprised 18 recognized species.…”

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Novosphingobium sediminicola sp. nov. isolated from freshwater sediment

Baek

Lim

Jin

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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A yellow-pigmented, Gram-negative, short rod-shaped, non-motile and non-spore-forming bacterial strain, designated HU1-AH51 T , was isolated from freshwater sediment and was characterized using a polyphasic approach, in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain HU1-AH51 T was shown to belong to the genus Novosphingobium, showing the highest level of sequence similarity with respect to Novosphingobium resinovorum NCIMB 8767 T (96.0 %), Novosphingobium naphthalenivorans TUT562 T (96.0 %) and Novosphingobium panipatense SM16 T (96.0 %). Strain HU1-AH51 T had a genomic DNA G+C content of 62.6 mol% and Q-10 as the predominant respiratory quinone. Furthermore, the major polyamine component (spermidine) in the cytoplasm and the presence of sphingoglycolipids suggested that strain HU1-AH51 T belongs to the family Sphingomonadaceae. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain HU1-AH51 T represents a novel species of the genus Novosphingobium, for which the name Novosphingobium sediminicola sp. nov. is proposed. The type strain is HU1-AH51 T (5LMG 24320 T 5KCTC 22311 T ).

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Novosphingobium taihuense sp. nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China

Abstract: Novosphingobium taihuense sp. nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China

Cited by 136 publications

References 28 publications

Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle

Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle

Novosphingobium piscinae sp. nov., isolated from a fish culture pond

Novosphingobium sediminicola sp. nov. isolated from freshwater sediment

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