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ABSTRACTHydrogen has been largely proposed as a possible alternative fuel for internal combustion engines. Its wide flammability range allows higher engine efficiency with leaner operation than conventional fuels, for both reduced toxic emissions and no CO 2 gases. Independently, Homogenous Charge Compression Ignition (HCCI) also allows higher thermal efficiency and lower fuel consumption with reduced NO X emissions when compared to Spark-Ignition (SI) engine operation. For HCCI combustion, a mixture of air and fuel is supplied to the cylinder and autoignition occurs from compression; engine is operated throttle-less and load is controlled by the quality of the mixture, avoiding the large fluid-dynamic losses in the intake manifold of SI engines. HCCI can be induced and controlled by varying the mixture temperature, either by Exhaust Gas Recirculation (EGR) or intake air pre-heating. A combination of HCCI combustion with hydrogen fuelling has great potential for virtually zero CO 2 and NO X emissions. Nevertheless, combustion on such a fast burning fuel with wide flammability limits and high octane number implies many disadvantages, such as control of backfiring and speed of autoignition and there is almost no literature on the subject, particularly in optical engines. Experiments were conducted in a singlecylinder research engine equipped with both Port Fuel Injection (PFI) and Direct Injection (DI) systems running at 1000 RPM. Optical access to in-cylinder phenomena was enabled through an extended piston and optical crown. Combustion images were acquired by a highspeed camera at 1° or 2° crank angle resolution for a series of engine cycles. Spark-ignition tests were initially carried out to benchmark the operation of the engine with hydrogen against gasoline. DI of hydrogen after intake valve closure was found to be preferable in order to overcome problems related to backfiring and air displacement from hydrogen's low density. HCCI combustion of hydrogen was initially enabled by means of a pilot port injection of n-heptane preceding the main direct injection of hydrogen, along with intake air preheating. Sole hydrogen fuelling HCCI was finally achieved and made sustainable, even at the low compression ratio of the optical engine by means of closed-valve DI, in synergy with air-pre-heating and negative valve overlap to promote intern...