2021
DOI: 10.1038/s41598-021-95073-9
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Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells

Abstract: Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells… Show more

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Cited by 39 publications
(26 citation statements)
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“…This might also explain the observed low effect of the particles on the metabolic activity of the cells. Moreover, these findings are in line with recent results for murine epithelial cell lines, where also no uptake of particles >0.2 µm was observed (Rudolph et al 2021 ). Particles with a size < 1 µm already constitute nano/submicromaterials.…”
Section: Resultssupporting
confidence: 92%
“…This might also explain the observed low effect of the particles on the metabolic activity of the cells. Moreover, these findings are in line with recent results for murine epithelial cell lines, where also no uptake of particles >0.2 µm was observed (Rudolph et al 2021 ). Particles with a size < 1 µm already constitute nano/submicromaterials.…”
Section: Resultssupporting
confidence: 92%
“…Plastic litter is an environmental concern as it is ubiquitous in water, air, and on land. Exposed to environmental conditions like changing temperature, mechanical stress, or UV radiation, it degrades into smaller particles. , Next to these particles, abrasion from any plastic product and particles generated in the size range below 5 mm are termed as microplastics . Its ubiquitous abundance and small size pose a risk to organisms and the human health due to particle ingestion and inhalation. , In the last decade, toxicity tests on various organisms and cells with different polymer types, sizes, and shapes were performed. Most of the studies focused thereby on spherical model particles, which are either pristine (plain) or functionalized. Although it is necessary to start investigations with well-defined particles, results of such studies do not represent the properties of real environmental microplastics …”
Section: Introductionmentioning
confidence: 99%
“…In previous studies, the uptake of small pristine poly(lactic acid) (PLA) and polystyrene (PS) particles (<0.5 μm) could be shown with intestinal epithelial cell lines (e.g., STC-1) after incubation in cell culture medium (ccm) with serum. 32,33 Hepatic epithelial cells (e.g., BNL CL. large PS particles (PSPs) with up to 3 μm in diameter.…”
Section: ■ Introductionmentioning
confidence: 99%
“…large PS particles (PSPs) with up to 3 μm in diameter. 33 The focus was on whether and how the history of a particle, i.e., the environment in which it has been incubated first, influences protein corona evolution and, therefore, the particles' interaction with cells (PCI). Therefore, PS particles (PSPs) were precoated with five different model proteins (bovine serum albumin, BSA, myoglobin, β-lactoglobulin, lysozyme, and fibrinogen) followed by a subsequent incubation in cell culture medium containing a cocktail of serum protein (i.e., fetal calf serum).…”
Section: ■ Introductionmentioning
confidence: 99%