NPTX1 inhibits pancreatic cancer cell proliferation and migration and enhances chemotherapy sensitivity by targeting RBM10

Wu, Jing; Liu, Gaifang; An, Kang; Shi, Linping

doi:10.3892/ol.2022.13275

Cited by 6 publications

(5 citation statements)

References 37 publications

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“…Our transcriptomic data align with existing studies that have identified genes like NPTX1, TRIM31, and CD82/KAI1 as potential therapeutic targets in various cancers [25][26][27][28][29][30][31][32][33]. NPTX1 has been implicated in inhibiting tumor growth across multiple types of cancer, acting through distinct signaling pathways.…”

Section: Promising Effects Of Upregulated Transcripts On Tumor Growth...supporting

confidence: 83%

From Fruit Waste to Medical Insight: The Comprehensive Role of Watermelon Rind Extract on Renal Adenocarcinoma Cellular and Transcriptomic Dynamics

Reddy,

Natarajan,

Nimmakayala

et al. 2023

IJMS

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Cancer researchers are fascinated by the chemistry of diverse natural products that show exciting potential as anticancer agents. In this study, we aimed to investigate the anticancer properties of watermelon rind extract (WRE) by examining its effects on cell proliferation, apoptosis, senescence, and global gene expression in human renal cell adenocarcinoma cells (HRAC-769-P) in vitro. Our metabolome data analysis of WRE exhibited untargeted phyto-constituents and targeted citrulline (22.29 µg/mg). HRAC-769-P cells were cultured in RPMI-1640 media and treated with 22.4, 44.8, 67.2, 88.6, 112, 134.4, and 156.8 mg·mL−1 for 24, 48, and 72 h. At 24 h after treatment, (88.6 mg·mL−1 of WRE) cell proliferation significantly reduced, more than 34% compared with the control. Cell viability decreased 48 and 72 h after treatment to 45% and 37%, respectively. We also examined poly caspase, SA-beta-galactosidase (SA-beta-gal), and wound healing activities using WRE. All treatments induced an early poly caspase response and a significant reduction in cell migration. Further, we analyzed the transcript profile of the cells grown at 44.8 mg·mL−1 of WRE after 6 h using RNA sequencing (RNAseq) analysis. We identified 186 differentially expressed genes (DEGs), including 149 upregulated genes and 37 downregulated genes, in cells treated with WRE compared with the control. The differentially expressed genes were associated with NF-Kappa B signaling and TNF pathways. Crucial apoptosis-related genes such as BMF, NPTX1, NFKBIA, NFKBIE, and NFKBID might induce intrinsic and extrinsic apoptosis. Another possible mechanism is a high quantity of citrulline may lead to induction of apoptosis by the production of increased nitric oxide. Hence, our study suggests the potential anticancer properties of WRE and provides insights into its effects on cellular processes and gene expression in HRAC-769-P cells.

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Section: Promising Effects Of Upregulated Transcripts On Tumor Growth...supporting

confidence: 83%

From Fruit Waste to Medical Insight: The Comprehensive Role of Watermelon Rind Extract on Renal Adenocarcinoma Cellular and Transcriptomic Dynamics

Reddy,

Natarajan,

Nimmakayala

et al. 2023

IJMS

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“…NPTX1 gene encodes the neuronal pentraxin 1, a member of the pentraxins family [ 31 ]. Recently, NPTX1 has been found to have decreased expression in diverse cancers [ 32 , 33 ], but its expression and impact in melanoma has not been defined yet. In this study, we discovered that circRPS5 knockdown could reduce NPTX1 expression.…”

Section: Discussionmentioning

confidence: 99%

Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis

et al. 2023

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Background Circular RNAs (circRNAs) have been reported to exert critical functions in tumorigenesis and development. However, the underlying mechanism by which circRNAs regulate melanoma progression remain to be elucidated. Methods The differentially expressed circRNAs were first identified by circRNA-seq, and circRNAs were validated via qRT-PCR and Sanger sequencing. Then, the impact of circRPS5, miR-151a and NPTX1 expression on the progression of melanoma cell were determined by gain- and loss-of-function assays. The relationship between circRPS5, miR-151a, and NPTX1 was predicted by StarBase website and authenticated by luciferase reporter assay. The melanoma cells-derived exosomes were characterized using nanoparticle tracking analysis (NTA) and western blot. Results CircRPS5 was significantly downregulated in melanoma tissues and cell lines. Functionally, circRPS5 suppressed the proliferation, migration, and invasion of melanoma cells, and induced cell cycle arrest and apoptosis in vitro. Mechanistically, circRPS5 harbor miR-151a, acting as miRNA sponge, and then miR-151a targeted the 3’-UTR of NPTX1. Finally, circRPS5 was mainly incorporated into exosomes to inhibit the progression of melanoma cells. Conclusions This finding reveal circRPS5 suppressed the progression of melanoma through miR-151a/NPTX1 pathway, and may provide a promising therapeutic strategies for melanoma.

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“…According to the related studies on the three candidate target genes, we found that SLC16A6 is a gene encoding monocarboxylic acid transporter 7, which is mainly related to lipid metabolism and length of zebrafish and human height (Karanth & Schlegel, 2018), but has not been related to the regulation of tumor cell proliferation and apoptosis. However, the functional mechanisms of DKK1 and NPTX1 in the regulation of tumor cell proliferation and apoptosis have been extensively proven (Glinka et al, 1998; Ren et al, 2013; Wang, Wang, et al, 2018; Wu et al, 2022; Zhao et al, 2018), and they seem to be more like the target genes of miR‐148a‐3p. Therefore, the regulatory effects of DKK1 and NPTX1 on the proliferation capacity of IGF‐GFs were preliminarily explored by cck‐8 experiment.…”

Section: Discussionmentioning

confidence: 99%

“…The structure of penetrins is very conserved in vertebrates, and they also have high homology sequences in phylogenetic distant species. Several lines of evidence have shown that NPTX1 exerts inhibitory effects on proliferation of different types of cancer cells, such as pancreatic cancer (Wu et al, 2022), breast cancer (Ye et al, 2021), head and neck cancer (Lu et al, 2020), hepatocellular carcinoma (Zhao et al, 2019), and colon cancer (Peng et al, 2018). In addition, previous studies have shown that NPTX1 acts as a target of different noncoding RNAs, including miRNAs.…”

Section: Discussionmentioning

confidence: 99%

miR‐148a‐3p regulates proliferation and apoptosis of idiopathic gingival fibroma by targeting NPTX1

et al. 2023

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ObjectiveIdiopathic gingival fibromatosis (IGF) is a rare heterogeneous disease that results in the progressive and diffuse hyperplasia of gingival tissues. MicroRNAs are implicated in the development and progression of various tumors. The present study aimed to explore the potential roles and mechanisms of miR‐148a‐3p in IGF.MethodsGingival fibroblasts (GFs) were transfected with miR‐148a‐3p mimics, miR‐148a‐3p inhibitors, or siNPTX1, and then, the proliferation and apoptosis of GFs and the expression of related genes were evaluated using Cell Counting Kit‐8 assays, 5‐ethynyl‐2′‐deoxyuridine assays, flow cytometry, reverse transcription‐quantitative polymerase chain reaction, and western blot analysis, respectively.ResultsmiR‐148a‐3p was highly expressed in GFs of IGF (IGF‐GFs) as compared with normal GFs (N‐GFs). Overexpression of miR‐148a‐3p promoted the proliferation and inhibited the apoptosis of N‐GFs, whereas downregulation of miR‐148a‐3p had the opposite effect in IGF‐GFs. Knockdown of NPTX1 reversed miR‐148a‐3p‐mediated effects in IGF‐GFs. Dual‐luciferase reporter assay confirmed that NPTX1 is a direct target of miR‐148a‐3p.ConclusionThese findings identify that miR‐148a‐3p could regulate cell proliferation and apoptosis by targeting NPTX1, providing new insights for the further study of the molecular mechanism and treatment of IGF.

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NPTX1 inhibits pancreatic cancer cell proliferation and migration and enhances chemotherapy sensitivity by targeting RBM10

Cited by 6 publications

References 37 publications

From Fruit Waste to Medical Insight: The Comprehensive Role of Watermelon Rind Extract on Renal Adenocarcinoma Cellular and Transcriptomic Dynamics

From Fruit Waste to Medical Insight: The Comprehensive Role of Watermelon Rind Extract on Renal Adenocarcinoma Cellular and Transcriptomic Dynamics

Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis

miR‐148a‐3p regulates proliferation and apoptosis of idiopathic gingival fibroma by targeting NPTX1

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