NR2F6, LOXL2 and DMBT1 expression in cervical cancer tissues, prognostic and clinicopathological implications

Abstract

Background. Detection of novel predictive markers for early detection of lymph nodes metastases in cervical cancer is very important. Disturbed NR2F6 expression was found in many cancers playing different roles according to cancer type. LOXL2 was incriminated in cancer progression and unfavorable survival in many cancer types. Decreased DMBT1 expression was found in many cancers during their progression. Aim of the present work was to assess expression of NR2F6, LOXL2 and DMBT1 in cervical cancer tissues using immunohistochemistry then correlating their expression with grade and stage of the tumor, occurrence of lymph nodes and distant metastases in addition to evaluating their prognostic roles. Materials and methods. We assessed the expression of NR2F6, LOXL2 and DMBT1 in samples retrieved from sixty two cervical cancer patients in cancer tissues and corresponding adjacent normal tissues and we correlated markers expression with clinical data, pathological findings and patients' prognosis. Results. High expression of NR2F6, LOXL2 and low expression of DMBT1 was up-regulated in cervical cancer tissues more than in adjacent non-neoplastic cervical tissues and was associated with high grade (p = 0.005), lymphovascular space involvement, advanced FIGO stage, resistance to chemotherapy, tumor recurrence, and shorter survival rates (p < 0.001). Conclusions. Up regulation of NR2F6, LOXL2 and down regulation of DMBT1 were associated with unfavorable pathological parameters, bad clinical findings and dismal outcome of cervical cancer patients. SOMMARIOContesto. La rilevazione di nuovi marcatori predittivi per la diagnosi precoce delle metastasi linfonodali nel cancro cervicale è molto importante. L'espressione distorta di NR2F6 è stata trovata in molti tumori che svolgono ruoli diversi a seconda del tipo di cancro. LOXL2 è stato incriminato nella progressione del cancro e nella sopravvivenza sfavorevole in molti tipi di cancro. La diminuzione dell'espressione di DMBT1 è stata trovata in molti tumori durante la loro progressione. Lo scopo del presente lavoro era valutare l'espressione di NR2F6, LOXL2 e DMBT1 nei tessuti del cancro cervicale mediante immunoistochimica, quindi correlando la loro espressione con il grado e lo stadio del tumore, la presenza di linfonodi e metastasi a distanza, oltre a valutare i loro ruoli prognostici. Materiali e metodi. Abbiamo valutato l'espressione di NR2F6, LOXL2 e DMBT1 in campioni prelevati da sessantadue pazienti con cancro cervicale nei tessuti tumorali e nei corrispondenti tessuti normali adiacenti e abbiamo correlato l'espressione dei marcatori con i dati clinici, i risultati patologici e la prognosi dei pazienti. Risultati. L'alta espressione di NR2F6, LOXL2 e la bassa espressione di DMBT1 erano sovraregolate nei tessuti del cancro cervicale più che nei tessuti cervicali adiacenti non neoplastici ed erano associate ad alto grado (p = 0,005), coinvolgimento dello spazio linfovascolare, stadio FIGO avanzato, resistenza a chemioterapia, recidiva del tumore e tassi di sopravvivenza pi...

Loop Electrosurgical Excision Procedure and Cold Knife Conization: which is the best? A large retrospective study

“…In a retrospective multi-institutional study, patients with high grade HR-HPV-negative cervical dysplasia have more favorable outcomes than patients with documented HR-HPV infection [33]. Furthermore, deregulation of some genes such as NR2F6, LOXL2 and DMBT1, could lead to unfavorable oncological outcomes in patients with cervical cancer [34]. A multicenter study has confirmed that preoperative conization could play a protective role in the risk of disease recurrence in patients with an early stage cervical cancer (IB1) [35].…”

Recurrent Non Hodgkin’s Lymphoma in the uterine cervix: a case report and a review of the literature

“…There is only little data regarding the prognosis of these patients, however the main prognostic factor seems to be the initial stage of the disease: as later is the diagnosis as worse is the prognosis. Due to the rarity of extranodal cervical NHL, there is no consensus in the literature concerning the optimal management of cervical extranodal NHL [10].…”