NRF2-directed PRPS1 upregulation to promote the progression and metastasis of melanoma

Xiong, Guohang; Ye, Feng; Yi, Xiaojia; Zhang, Xuedan; Li, Xiaoyü; Yang, Lijuan; Yi, Zihan; Sai, Buqing; Yang, Zhe; Zhang, Qiao; Kuang, Yingmin; Zhu, Yuechun

doi:10.3389/fimmu.2022.989263

Cited by 3 publications

(3 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been found that PRPS1 and PRPS2 play important regulatory roles in different malignant tumors. PRPS1 is overexpressed in melanoma and accelerates tumor progression by promoting cell growth, metastasis, and suppressing cell apoptosis 8 . PRPS2 is the target of myeloid proliferative oncogene (c‐Myc), which changes with c‐Myc expression 9 .…”

Section: Introductionmentioning

confidence: 99%

“…PRPS1 is overexpressed in melanoma and accelerates tumor progression by promoting cell growth, metastasis, and suppressing cell apoptosis. 8 PRPS2 is the target of myeloid proliferative oncogene (c‐Myc), which changes with c‐Myc expression. 9 Previously, the expression levels of PRPS2 vary in different tumor tissues, and its related mechanism is preliminarily discussed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulatory mechanism and expression level of PRPS2 in lung cancer

Meng,

Zhang,

et al. 2024

Thoracic Cancer

View full text Add to dashboard Cite

BackgroundLung cancer, with high morbidity and mortality, is the commonest respiratory system neoplasm, which seriously endangers the life safety of patients. In this study, the effect of PRPS2 on cell progression was preliminarily investigated.MethodsImmunohistochemical staining, western blot and reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) were performed to verify the expression level of PRPS2 in lung cancer. Lung cancer cell lines with stable downregulation of PRPS2 were constructed in A549 cells and NCIH460 cells. The function of PRPS2 silencing on the proliferation ability was verified by the EdU and cell colony formation experiment. Scratch and transwell tests were conducted to verify the role of PRPS2 silencing on the migratory and invasive ability of cells. The impact of PRPS2 silencing on cell apoptosis and cell cycle was verified by flow cytometry test. The effects of PRPS2 silencing on apoptosis‐associated proteins were assessed by western blot assay. The function of PRPS2 silencing on tumor growth in vivo was studied through xenograft tumor experiment.ResultsIn comparison with normal tissues, PRPS2 was upregulated in lung cancer tissues. PRPS2 knockdown notably hindered the migratory ability, invasive ability and proliferation, but accelerated cell apoptosis. In vivo experiments confirmed that PRPS2 silencing blocked the growth of transplanted tumors.ConclusionIn lung cancer, PRPS2 silencing suppressed the malignant progression, indicating that PRPS2 might be a novel biomarker for lung cancer treatment and diagnosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulatory mechanism and expression level of PRPS2 in lung cancer

Meng,

Zhang,

et al. 2024

Thoracic Cancer

View full text Add to dashboard Cite

show abstract

“…Previous studies primarily focused on decoding melanoma metastasis from a single molecule or single pathway perspective [ 10 – 12 ]. However, the tumor microenvironment (TME) of melanoma is complex and dynamic, with intricate crosstalk between cells, resulting in significant losses of key metastasis-related information.…”

Section: Introductionmentioning

confidence: 99%

Decoding the metastatic potential and optimal postoperative adjuvant therapy of melanoma based on metastasis score

Shen,

Song,

Wang

et al. 2023

Cell Death Discov.

View full text Add to dashboard Cite

Metastasis is a formidable challenge in the prognosis of melanoma. Accurately predicting the metastatic potential of non-metastatic melanoma (NMM) and determining effective postoperative adjuvant treatments for inhibiting metastasis remain uncertain. In this study, we conducted comprehensive analyses of melanoma metastases using bulk and single-cell RNA sequencing data, enabling the construction of a metastasis score (MET score) through diverse machine-learning algorithms. The reliability and robustness of the MET score were validated using various in vitro assays and in vivo models. Our findings revealed a distinct molecular landscape in metastatic melanoma characterized by the enrichment of metastasis-related pathways, intricate cell–cell communication, and heightened infiltration of pro-angiogenic tumor-associated macrophages compared to NMM. Importantly, patients in the high MET score group exhibited poorer prognoses and an immunosuppressive microenvironment, featuring increased infiltration of regulatory T cells and decreased infiltration of CD8+ T cells, compared to the low MET score patient group. Expression of PD-1 was markedly higher in patients with low MET scores. Anti-PD-1 (aPD-1) therapy profoundly affected antitumor immunity activation and metastasis inhibition in these patients. In summary, our study demonstrates the effectiveness of the MET score in predicting melanoma metastatic potential. For patients with low MET scores, aPD-1 therapy may be a potential treatment strategy to inhibit metastasis. Patients with high MET scores may benefit from combination therapies.

show abstract