NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family

Higuera, José Javier; Calatrava, Victoria; González, Zaira; Mariscal, Vicente; Siverio, José M.; Fernández, Emilio; Galván, Aurora

doi:10.3390/agronomy6010020

Cited by 8 publications

(5 citation statements)

References 53 publications

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“…This structure is also present in the predicted topology for ZosmaNRT2 with a central cytosolic loop of 31 amino acids (Figure 1). Furthermore, in all members of the NNP family, it has been experimentally shown that N and C-terminal domains and the central loop fall on the cytosolic side of the plasma membrane [33,34,35]. This feature is also preserved in the proposed topology for ZosmaNRT2 (Figure 1).…”

Section: Discussionmentioning

confidence: 89%

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Rubio

Díaz‐García

Amorim‐Silva

et al. 2019

IJMS

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One of the most important adaptations of seagrasses during sea colonization was the capacity to grow at the low micromolar nitrate concentrations present in the sea. In contrast to terrestrial plants that use H+ symporters for high-affinity NO3− uptake, seagrasses such as Zostera marina L. use a Na+-dependent high-affinity nitrate transporter. Interestingly, in the Z. marina genome, only one gene (Zosma70g00300.1; NRT2.1) is annotated to this function. Analysis of this sequence predicts the presence of 12 transmembrane domains, including the MFS domains of the NNP transporter family and the “nitrate signature” that appears in all members of the NNP family. Phylogenetic analysis shows that this sequence is more related to NRT2.5 than to NRT2.1, sharing a common ancestor with both monocot and dicot plants. Heterologous expression of ZosmaNRT2-GFP together with the high-affinity nitrate transporter accessory protein ZosmaNAR2 (Zosma63g00220.1) in Nicotiana benthamiana leaves displayed four-fold higher fluorescence intensity than single expression of ZosmaNRT2-GFP suggesting the stabilization of NRT2 by NAR2. ZosmaNRT2-GFP signal was present on the Hechtian-strands in the plasmolyzed cells, pointing that ZosmaNRT2 is localized on the plasma membrane and that would be stabilized by ZosmaNAR2. Taken together, these results suggest that Zosma70g00300.1 would encode a high-affinity nitrate transporter located at the plasma membrane, equivalent to NRT2.5 transporters. These molecular data, together with our previous electrophysiological results support that ZosmaNRT2 would have evolved to use Na+ as a driving ion, which might be an essential adaptation of seagrasses to colonize marine environments.

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Section: Discussionmentioning

confidence: 89%

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Rubio

Díaz‐García

Amorim‐Silva

et al. 2019

IJMS

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“…In land plants, three different gene families of nitrate transporters have been identified, NRT1, NRT2, and NRT3 ( Plett et al., 2010 ), all of which symport NO 3 − and protons, with low or high affinity for NO 3 − ( Pinton et al., 2016 ). In addition, the genome of the unicellular green alga Chlamydomonas reinhardtii contains six NRT2 genes ( Higuera et al., 2016 ), and the unicellular red alga Porphyridium purpureum contains two NRT2 genes ( Figure 3 ). Therefore, the presence of a single-copy NRT2 gene ( Figure 2 ; Kakinuma et al., 2008 ; Brawley et al., 2017 ; Li et al., 2019b ) is unique to multicellular Bangiales.…”

Section: Resultsmentioning

confidence: 99%

Heat stress memory differentially regulates the expression of nitrogen transporter genes in the filamentous red alga ‘Bangia’ sp. ESS1

Sato,

Khoa,

Mikami

2024

Front. Plant Sci.

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IntroductionTo withstand high temperatures that would be lethal to a plant in the naïve state, land plants must establish heat stress memory. The acquisition of heat stress tolerance via heat stress memory in algae has only been observed in the red alga ‘Bangia’ sp. ESS1.MethodsIn this study, we further evaluated the intrinsic ability of this alga to establish heat stress memory by monitoring hydrogen peroxide (H2O2) production and examining the relationship between heat stress memory and the expression of genes encoding nitrogen transporters, since heat stress generally reduces nitrogen absorption. Next, genes encoding nitrogen transporters were selected from our unpublished transcriptome data of ‘Bangia’ sp. ESS1.ResultsWe observed a reduction in H2O2 content when heat stress memory was established in the alga. In addition, six ammonium transporter genes, a single-copy nitrate transporter gene and two urea transporter genes were identified. Two of these nitrogen transporter genes were induced by heat stress but not by heat stress memory, two genes showed heat stress memory–dependent expression, and one gene was induced by both treatments. Heat stress memory therefore differentially regulated the expression of the nitrogen transporter genes by reducing heat stress–inducible gene expression and inducing heat stress memory–dependent gene expression.DiscussionThese findings point to the functional diversity of nitrogen transporter genes, which play different roles under various heat stress conditions. The characteristic effects of heat stress memory on the expression of individual nitrogen transporter genes might represent an indispensable strategy for reducing the threshold of sensitivity to recurrent high-temperature conditions and for maintaining nitrogen absorption under such conditions in ‘Bangia’ sp. ESS1.

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“…Other transporters (NRT2. [3][4][5][6] in the NRT2 family are low-affinity nitrate transporters [36,39]. NAR1 transporters only play a role in nitrite and bicarbonate transportation; hence, we decided to choose NRT1, NRT2.1 and NRT2.2 genes from nitrate/nitrite transporter families for expression study as these three seem to play the role of the main nitrate transporters.…”

Section: Discussionmentioning

confidence: 99%

Light-Dependent Nitrate Removal Capacity of Green Microalgae

Rani

Maróti

2022

IJMS

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In the present study, Chlamydomonas sp. MACC-216 was used to investigate total nitrate removal in TAP medium with sodium nitrate as the sole nitrogen source under several light conditions made up of permuted combinations of three light colors (referred to as blue, red, and white light) and three light intensities (50 µmol m−2 s−1, 100 µmol m−2 s−1, and 250 µmol m−2 s−1). It was observed that nitrate removal efficiency is influenced by light color as well as light intensity. Additionally, Chlamydomonas sp. MACC-216 was cultivated in synthetic wastewater under four light conditions, namely, Blue 250, Blue 125 + Red 125, Red 250, and White 250, where it showed the highest nitrate removal efficiency and nitrate reductase activity under the Blue 125 + Red 125 light condition. To observe the impact of light color on the nitrate removal capacity of Chlamydomonas sp. MACC-216, the expression of five genes participating in nitrate transport and reduction (NRT1, NRT2.1. NRT2.2, NIA, and MCP) was also analyzed; these genes showed the highest expression under the Blue 125 + Red 125 light condition. Based on the above-mentioned findings, the blue + red light combination emerged as a promising light combination for nitrate removal. Hence, our study suggests the importance of the blue + red light combination together with high light intensity, as the optimal light condition for nitrate removal from synthetic wastewater in comparison to other monochromatic lights with high light intensity.

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NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family

Cited by 8 publications

References 53 publications

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Heat stress memory differentially regulates the expression of nitrogen transporter genes in the filamentous red alga ‘Bangia’ sp. ESS1

Light-Dependent Nitrate Removal Capacity of Green Microalgae

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