In order to meet milling industry and grain producers' requirements, wheat grain needs to possess good technological quality, and high and stable grain production. This study analyzed 30 varieties of winter wheat over four growing seasons (2015/16, 2016/17, 2017/18, and 2018/19) at the location Rimski Šančevi, Serbia. The studied traits were under the control of genotype, environment, and their interaction. The analysis of variance (ANOVA) revealed that the influence of the factors varied depending on the studied trait. Thousand grain weight showed the highest variability depending on genotype influence, protein content and sedimentation value that were mostly affected by environmental conditions, while the genotype and environment interaction had the greatest impact on hectoliter weight and grain yield. Using principal component analysis significant positive relationship was observed between protein content and sedimentation value, and between thousand grain weight and hectoliter weight. It was also shown that yield and thousand grain weight, sedimentation value and hectoliter weight are positively related. A strong negative association was noted between grain yield and protein content. The protein content and hectoliter weight did not show any dependency. All analyzed traits are significant for breeding programs, emphasizing the importance of developing high-quality varieties with high yield potential and tolerance to adverse environmental conditions.