NSCs Migration Promoted and Drug Delivered Exosomes‐Collagen Scaffold via a Bio‐Specific Peptide for One‐Step Spinal Cord Injury Repair

Zhang, Lulu; Fan, Cunyi; Hao, Wangping; Zhuang, Yan; Liu, Xiru; Zhao, Yannan; Chen, Bing; Xiao, Zhifeng; Chen, Yanyan; Dai, Jianwu

doi:10.1002/adhm.202001896

Cited by 66 publications

(52 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Natural materials can show the advantages of inherent environmental response characteristics through degradation and remodeling compared with synthetic polymers. In recent years, collagen and chitosan materials have been widely used in various tissue engineering and biomedical applications for the advantage of biodegradability, good biocompatibility, low antigenicity and absence of pyrogenic effects [ 5 , 46 , 48 , 49 ]. Collagen and chitosan were also selected as materials for 3D printing and promoting nerve regeneration after SCI in this study.…”

Section: Discussionmentioning

confidence: 99%

Integrated printed BDNF/collagen/chitosan scaffolds with low temperature extrusion 3D printer accelerated neural regeneration after spinal cord injury

Liu

Chen

et al. 2021

Regenerative Biomaterials

View full text Add to dashboard Cite

Recent studies have shown that 3D printed scaffolds integrated with growth factors can guide the growth of neurites and promote axon regeneration at the injury site. However, heat, organic solvents or cross-linking agents used in conventional 3D printing reduce the biological activity of growth factors. Low temperature 3D printing can incorporate growth factors into the scaffold and maintain their biological activity. In this study, we developed a collagen/chitosan scaffold integrated with brain-derived neurotrophic factor (3D-CC-BDNF) by low temperature extrusion 3D printing as a new type of artificial controlled release system, which could prolong the release of BDNF for the treatment of SCI. 8 weeks after the implantation of scaffolds in the transected lesion of T10 of the spinal cord, 3D-CC-BDNF significantly ameliorate locomotor function of the rats. Consistent with the recovery of locomotor function, 3D-CC-BDNF treatment could fill the gap, facilitate nerve fiber regeneration, accelerate the establishment of synaptic connections and enhance remyelination at the injury site.

show abstract

Section: Discussionmentioning

confidence: 99%

Integrated printed BDNF/collagen/chitosan scaffolds with low temperature extrusion 3D printer accelerated neural regeneration after spinal cord injury

Liu

Chen

et al. 2021

Regenerative Biomaterials

View full text Add to dashboard Cite

show abstract

“…In a previous study, neurons of spinal cord injury also increased at 8 days after SCI modeling ( 35 ). Moreover, endogenous neural stem cells and ependymal stem cells could differentiate into neurons after spinal cord injury ( 36 , 37 ). Lineage tracing experiments have shown that it is solely the ependymal cell population that is capable of generating neurospheres in vitro , and this hallmark of NSC potential remains restricted to the ependymal population following SCI ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota Disorders Promote Inflammation and Aggravate Spinal Cord Injury Through the TLR4/MyD88 Signaling Pathway

et al. 2021

View full text Add to dashboard Cite

Background: In spinal cord injury (SCI), systemic inflammation and the death of nerve cells in the spinal cord are life threatening. The connection between gut microbiota and signaling pathways has been a hot research topic in recent years. The Toll-like receptor 4/Myeloid differentiation factor 88 (TLR4/MyD88) signaling pathway is closely related to the inflammatory response. This study explored whether the gut microbiota imbalance could affect the TLR4/MyD88 signaling pathway to regulate SCI to provide a new basis for SCI research and treatment.Methods: An SCI model was constructed to study the influence on the injury of gut microbiota. 16S amplicon sequencing was used to identify the diversity and abundance of gut microbes. Fecal microbiota transplantation was performed in mice with SCI. ELISA was used to detect the serum levels of pro-inflammatory and anti-inflammatory factors in mice. Hematoxylin and eosin staining was used to observe SCI in mice. Immunofluorescence was used to detect the rates of loss glial fibrillary acidic protein (GFAP), neuronal nuclear protein (NeuN), and ionized calcium-binding adapter molecule 1 (IBA1) in the spinal cord as indicators of apoptosis. The expression of the TLR4/MyD88 signaling pathway was detected by qRT-PCR and western blotting.Results: Significant differences were observed in the gut microbiota of SCI mice and normal mice. The gut microbiota of SCI mice was imbalanced. The levels of pro-inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in SCI mice were increased, as was the level of the toxic induced nitric oxide synthase. The levels of anti-inflammatory factors IL-4, transforming growth factor-β, and IL-10 were decreased, as was the level of arginase-1. The apoptosis rates of GFAP, NeuN, and IBA1 were increased. The TLR4/MyD88 signaling pathway was activated. In the SCI group, inflammation increased after fecal transplantation, apoptosis of GFAP, NeuN, and IBA1 increased, and SCI was more serious.Conclusion: The TLR4/MyD88 signaling pathway promotes the death of nerve cells by inducing inflammation. Gut microbiota dysregulation can lead to aggravated SCI by activating the TLR4/MyD88 signaling pathway.

show abstract

“…Because of this characteristic, MExos can be used as an effective drug carrier. Researchers utilized MExos to deliver PTX via a bio-specific peptide to promote nerve regeneration and reduce scar tissue deposition, which showed excellent performance in the re-establishment of motor skills after complete SCI in rats, such that this method can repair SCI in one step (Zhang et al, 2021).…”

Section: Exosomal Mirnas Improve Sci Recoverymentioning

confidence: 99%

Emerging Exosomes and Exosomal MiRNAs in Spinal Cord Injury

Feng

Zhang

Zhu

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Acute spinal cord injury (SCI) is a serious traumatic event to the spinal cord with considerable morbidity and mortality. This injury leads to short- and long-term variations in the spinal cord, and can have a serious effect on the patient’s sensory, motor, or autonomic functions. Due to the complicated pathological process of SCI, there is currently no successful clinical treatment strategy. Exosomes, extracellular vesicles (EVs) with a double-layer membrane structure of 30–150 nm diameter, have recently been considered as critical mediators for communication between cells and tissues by transferring proteins, lipids, and nucleic acids. Further studies verified that exosomes participate in the pathophysiological process of several diseases, including cancer, neurodegenerative diseases, and cardiovascular diseases, and could have a significant impact in their treatment. As natural carriers of biologically active cargos, exosomes have emerged as pathological mediators of SCI. In this review article, we critically discuss the functions of exosomes as intracellular mediators and potential treatments in SCI and provide an outlook on future research.

show abstract

NSCs Migration Promoted and Drug Delivered Exosomes‐Collagen Scaffold via a Bio‐Specific Peptide for One‐Step Spinal Cord Injury Repair

Cited by 66 publications

References 52 publications

Integrated printed BDNF/collagen/chitosan scaffolds with low temperature extrusion 3D printer accelerated neural regeneration after spinal cord injury

Integrated printed BDNF/collagen/chitosan scaffolds with low temperature extrusion 3D printer accelerated neural regeneration after spinal cord injury

Gut Microbiota Disorders Promote Inflammation and Aggravate Spinal Cord Injury Through the TLR4/MyD88 Signaling Pathway

Emerging Exosomes and Exosomal MiRNAs in Spinal Cord Injury

Contact Info

Product

Resources

About