Abstract:The
nongenetic modification of cell membranes with proteins
is
a straightforward way of cellular engineering. In these processes,
it is important to specifically address the proteins to liquid-ordered
(Lo) or liquid-disordered (Ld) domains as this can largely affect
their biological functions. Herein, we report a cholesterol analogue
(CHIM) with a nitrilotriacetic acid (NTA) headgroup, named CHIM-NTA.
CHIM-NTA integrates into lipid membranes similar to the widely used
phospholipid-derived DGS–NTA and, when loa… Show more
“…The cell membrane skeleton is a phospholipid bilayer structure allowing the spontaneous insertion of synthetic materials with hydrophobic anchors or “tails” driven by the hydrophobic effect. 71–73 The commonly used hydrophobic anchors include lipids (phospholipids 21,26,73–80 and cholesterols 81–92 ), alkane chains 93–98 and oleyl chains. 99–105 They can be categorized into single and multiple anchors based on the number of hydrophobic anchors.…”
This review offers a survey of recent advancements in the modification of mammalian cell surfaces through the use of synthetic molecules and concludes by addressing the present challenges and potential opportunities in this rapidly expanding field.
“…The cell membrane skeleton is a phospholipid bilayer structure allowing the spontaneous insertion of synthetic materials with hydrophobic anchors or “tails” driven by the hydrophobic effect. 71–73 The commonly used hydrophobic anchors include lipids (phospholipids 21,26,73–80 and cholesterols 81–92 ), alkane chains 93–98 and oleyl chains. 99–105 They can be categorized into single and multiple anchors based on the number of hydrophobic anchors.…”
This review offers a survey of recent advancements in the modification of mammalian cell surfaces through the use of synthetic molecules and concludes by addressing the present challenges and potential opportunities in this rapidly expanding field.
“…11 b) showing the fusogenic ability of the IL. On the side, an interesting addition brought into the picture by this team is the synthesis, characterization and applications of a class of versatile and clickable cholesterol-based imidazolium salt (Rakers et al 2018 ; Zheng et al 2023 ). Fig.…”
In the past 25 years, a vast family of complex organic salts known as room-temperature ionic liquids (ILs) has received increasing attention due to their potential applications. ILs are composed by an organic cation and either an organic or inorganic anion, and possess several intriguing properties such as low vapor pressure and being liquid around room temperature. Several biological studies flagged their moderate-to-high (cyto)-toxicity. Toxicity is, however, also a synonym of affinity, and this boosted a series of biophysical and chemical-physical investigations aimed at exploiting ILs in bio-nanomedicine, drug-delivery, pharmacology, and bio-nanotechnology. Several of these investigations focused on the interaction between ILs and lipid membranes, aimed at determining the microscopic mechanisms behind their interaction. This is the focus of this review work. These studies have been carried out on a variety of different lipid bilayer systems ranging from 1-lipid to 5-lipids systems, and also on cell-extracted membranes. They have been carried out at different chemical-physical conditions and by the use of a number of different approaches, including atomic force microscopy, neutron and X-ray scattering, dynamic light scattering, differential scanning calorimetry, surface quartz microbalance, nuclear magnetic resonance, confocal fluorescence microscopy, and molecular dynamics simulations. The aim of this “2023 Michèle Auger Award” review work is to provide the reader with an up-to-date overview of this fascinating research field where “ILs meet lipid bilayers (aka biomembranes),” with the aim to boost it further and expand its cross-disciplinary edges towards novel high-impact ideas/applications in pharmacology, drug delivery, biomedicine, and bio-nanotechnology.
Enzymatic reactions can consume endogenous nutrients of tumor and produce cytotoxic species, therefore are promising tools for treating malignant tumors. Inspired by nature that enzymes are compartmentalized in membranes to achieve high reaction efficiency and separate biological processes with the environment, we develop liposomal nanoreactors that can perform enzymatic cascade reactions in the aqueous nanoconfinement of liposome. The nanoreactors effectively inhibited tumor growth in vivo by consuming tumor nutrients (glucose and oxygen) and producing highly cytotoxic hydroxyl radicals (·OH). Co‐compartmentalization of glucose oxidase (GOx) and horseradish peroxidase (HRP) in liposomes could increase local concentration of intermediate product H2O2 as well as the acidity due to the generation of gluconic acid by GOx. Both H2O2 and acidity accelerate the second‐step reaction by HRP, hence improving the overall efficiency of the cascade reaction. The biomimetic compartmentalization of enzymatic tandem reactions in biocompatible liposomes provides a promising direction for developing catalytic nanomedicines in anti‐tumor therapy.
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