NtMYB4 and NtCHS1 Are Critical Factors in the Regulation of Flavonoid Biosynthesis and Are Involved in Salinity Responsiveness

Chen, Shuai; Wu, Fengyan; Li, Yiting; Qian, Yanli; Pan, Xuhao; Li, Fengxia; Wang, Yuanying; Wu, Zhenying; Fu, Chunxiang; Lin, Haoran; Yang, Aiguo

doi:10.3389/fpls.2019.00178

Cited by 114 publications

(74 citation statements)

References 58 publications

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“…Previous studies have shown that flavonoids are powerful antioxidants and are involved in abiotic stress responses (Martinez et al, 2016;Chen et al, 2019). We found that ROS accumulation was decreased in NtMYB12 transgenic tobacco under low Pi treatment ( Figure 5), which was consistent with the FIGURE 7 | Quantification of flavonol concentration and relative transcript levels of majority genes encoding flavonoid biosynthetic enzymes in WT and 35S: NtMYB12 plants under HP and LP conditions.…”

Section: Overexpression Of Ntmyb12 Enhances Plant Resistance To Low Psupporting

confidence: 87%

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

et al. 2020

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Phosphorus (P) is an essential macronutrient for plant growth and development. The concentration of flavonol, a natural plant antioxidant, is closely related to phosphorus nutritional status. However, the regulatory networks of flavonol biosynthesis under low Pi stress are still unclear. In this study, we identified a PFG-type MYB gene, NtMYB12, whose expression was significantly up-regulated under low Pi conditions. Overexpression of NtMYB12 dramatically increased flavonol concentration and the expression of certain flavonol biosynthetic genes (NtCHS, NtCHI, and NtFLS) in transgenic tobacco. Moreover, overexpression of NtMYB12 also increased the total P concentration and enhanced tobacco tolerance of low Pi stress by increasing the expression of Pht1-family genes (NtPT1 and NtPT2). We further demonstrated that NtCHS-overexpressing plants and NtPT2-overexpressing plants also had increased flavonol and P accumulation and higher tolerance to low Pi stress, showing a similar phenotype to NtMYB12-overexpressing transgenic tobacco under low Pi stress. These results suggested that tobacco NtMYB12 acts as a phosphorus starvation response enhancement factor and regulates NtCHS and NtPT2 expression, which results in increased flavonol and P accumulation and enhances tolerance to low Pi stress.

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Section: Overexpression Of Ntmyb12 Enhances Plant Resistance To Low Psupporting

confidence: 87%

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

et al. 2020

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“…Flavonoids are an essential type of secondary metabolites, which play a vital role in plants against oxidative damage caused by ROS and MDA during abiotic stress [ 98 ], which help to enhance salt tolerance when accumulated [ 99 , 100 ]. In the current study, we observed the upregulation of four DEGs involved in flavonoid, flavone, and flavonol biosynthesis ( c82620.graph_c0 , c86889.graph_c0 , c87219.graph_c0 , and c67471.graph_c0 ) in V11E0022 under salt stress.…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, we observed the upregulation of four DEGs involved in flavonoid, flavone, and flavonol biosynthesis ( c82620.graph_c0 , c86889.graph_c0 , c87219.graph_c0 , and c67471.graph_c0 ) in V11E0022 under salt stress. Previously conducted studies also showed that salt stress induced the flavonoids accumulation in plants and enhanced their quality [ 98 , 101 ]. The physiological results of the current study also showed higher content of flavonoid in V11E0022 under salt treatment in comparison to other counterparts, and this is due to the overexpression of the genes involved in the flavonoid biosynthesis pathway.…”

Section: Discussionmentioning

confidence: 99%

Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica

Kumar

Yang

et al. 2020

Antioxidants

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Abiotic stress, such as drought and salinity, severely affect the growth and yield of many plants. Oenanthe javanica (commonly known as water dropwort) is an important vegetable that is grown in the saline-alkali soils of East Asia, where salinity is the limiting environmental factor. To study the defense mechanism of salt stress responses in water dropwort, we studied two water dropwort cultivars, V11E0022 and V11E0135, based on phenotypic and physiological indexes. We found that V11E0022 were tolerant to salt stress, as a result of good antioxidant defense system in the form of osmolyte (proline), antioxidants (polyphenols and flavonoids), and antioxidant enzymes (APX and CAT), which provided novel insights for salt-tolerant mechanisms. Then, a comparative transcriptomic analysis was conducted, and Gene Ontology (GO) analysis revealed that differentially expressed genes (DEGs) involved in the carbohydrate metabolic process could reduce oxidative stress and enhance energy production that can help in adaptation against salt stress. Similarly, lipid metabolic processes can also enhance tolerance against salt stress by reducing the transpiration rate, H2O2, and oxidative stress. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that DEGs involved in hormone signals transduction pathway promoted the activities of antioxidant enzymes and reduced oxidative stress; likewise, arginine and proline metabolism, and flavonoid pathways also stimulated the biosynthesis of proline and flavonoids, respectively, in response to salt stress. Moreover, transcription factors (TFs) were also identified, which play an important role in salt stress tolerance of water dropwort. The finding of this study will be helpful for crop improvement under salt stress.

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“…The consequences indicated that the binding capacity between MYB DETFs and GS was stronger in Bluemoon than that in Balin, and this could be one of the reasons that promoted N assimilation in GS/GOGAT cycle and enhanced the NUE of Bluemoon in a complex signaling community at low N condition. Previous research have shown that R2R3-MYB TFs possess the features in improving the tolerance of Arabidopsis to abiotic stress [43]. However, the N-mediated mechanisms for these R2R3-MYB TFs in KB warrant in addition exploration.…”

Section: N Metabolism On the Physiological And Molecular Regulation Imentioning

confidence: 99%

Comparative Study on Nitrogen Assimilation and Gene Regulation of different Kentucky bluegrass Cultivars in response to Nitrate supply

Sun

Chen

2020

Preprint

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Background: Kentucky bluegrass (Poa pratensis L) is one of the most popular cool-season turfgrass worldwide, but the mechanisms of this species in response to low nitrogen (N) still remain unclear. In this study, we characterized two cultivars ‘Bluemoon’ and ‘Balin’ distinctly in morphological, chromosomal, physiological and molecular attributes to N supply.Results: Bluemoon was more tolerant to low N than Balin by exhibiting higher turf quality (TQ), photosynthetic ability, activities of N reductases and synthetases and nitrogen use efficiency (NUE). Gene expression profiling showed that there were 968 and 336 differentially expressed genes (DEGs) after eliminating genetic background differences in Bluemoon and Balin respectively during low N stress, and these DEGs highly enriched in ‘Nitrogen metabolism’, ‘Pyruvate metabolism’ and ‘Carbon fixation in photosynthetic’ pathways. The identified genes related to carbon (C) metabolism highly expressed in Bluemoon, which could generate more NADPH then result in more N reduction comparing with Balin. Moreover, R2R3-MYB transcription factors were predicted to bind the promoter of GS to enhance the efficiency of GS/GOGAT cycle.Conclusion: These results could be crucial molecular regulations for improving the tolerance to low N and NUE in Bluemoon. The climate and geography in origins may shape the N assimilation patterns in Kentucky bluegrass via long-term domestication. Taken together, the findings help elucidate the low N tolerance mechanisms in Kentucky bluegrass and would be valuable for the genetic improvement of NUE aiming to promote low-input turfgrass management.

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NtMYB4 and NtCHS1 Are Critical Factors in the Regulation of Flavonoid Biosynthesis and Are Involved in Salinity Responsiveness

Cited by 114 publications

References 58 publications

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica

Comparative Study on Nitrogen Assimilation and Gene Regulation of different Kentucky bluegrass Cultivars in response to Nitrate supply

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