Nuclear Accumulation of Filamentous Herpes Simplex Virus DNA Late during the Replicative Cycle

Luetzeler, Josef; Heine, Ursula

doi:10.1159/000148991

Cited by 9 publications

(8 citation statements)

References 9 publications

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“…Capsids and nucleocapsids occur along the edge of the coarse matrix (Kanich & Craighead 1972, Iwasaki et al 1973, Smith & d e Harven 1973, Fons & Albrecht 1986, the site of viral DNA production (Fong 1982, Wolber et al 1988. At this site in CMV infections, fibrils of viral DNA (Fong 1982) form fibrillar, vermiform, or tadpole-shaped structures that enter the capsids (Ruebner et al 1965, Kanich & Craighead 1972, Fong et al 1980, Cavallo et al 1981, whereas in HSV infections encapsidation does not occur in association with specific inclusions (Luetzeler & Heine 1978, Whealy et al 1991. The strands or rods of dark material in June samples, and reticulated coarse granular inclusions in March samples, of Ostrea angasi may therefore be involved in DNA synthesis prior to encapsidation.…”

Section: Discussionmentioning

confidence: 99%

Replication of herpes-like viruses in haemocytes of adult flat oysters Ostrea angasi:an ultrastructural study

Pm¹,

Thorne²

1997

Dis. Aquat. Org.

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Eosinophilic intranuclear inclusions are reported from agranular haemocytes and eosinophilic granulocytes of Ostrea angasi (Sowerby, 1871). Ultrastructurally, ovoid to hexagonal capsids 98 ? 4 nm (range 90 to 105 nm; n = 60) in diameter formed around a n ovoid, fine granular inclusion at the periphery of the nucleus Strands and rod-like configurations of dark material near the centre of the nucleus developed into a coarse granular matrix along the edge of which capsids acquired a fibrillar, vermiform or rod-like core. Many capsids appeared to degenerate along the nuclear periphery Those passing into the cytoplasm acquired an envelope in the perinuclear space, and cytoplasmic nucleocapsids acquired an envelope when passing into endoplasm~c reticulum or cytoplasmic vesicles. The few virions observed had tails and were 135 to 140 nm across. Nuclear stages of replication are more similar to cytomegaloviruses (Betaherpesvirinae) than to herpes simplex viruses (Alphaherpesvirinae). Incomplete replication and the low level of virion production are attributed to sampling in late summer and winter when temperatures are sub-optimal.

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Section: Discussionmentioning

confidence: 99%

Replication of herpes-like viruses in haemocytes of adult flat oysters Ostrea angasi:an ultrastructural study

Pm¹,

Thorne²

1997

Dis. Aquat. Org.

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“…It is likely that virus stocks prepared either in animM tissues or in tissue cultures may be important contributing factors for the differences in ultrastruetural developments. In herpes simplex virus type 1-infected WI-38 cells, Lus~znL~n (14) observed similar fibrils in the nuclei and identified them as DNAeontMning nucleoprotein. The fiMillar structures in GPCMV infected salivary gland duct cells may also represent DNA-eontMning core materials.…”

Section: Diseussionmentioning

confidence: 96%

Ultrastructural development and persistence of guinea pig cytomegalovirus in duct cells of guinea pig submaxillary gland

Fong

Bia

Hsiung

1980

Archives of Virology

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Salivary glands from Hartley guinea pigs were experimentally infected with guinea pig cytomegalovirus (GPCMV) and examined by light and electron microscopy at different time intervals. Characteristic intranuclear and intracytoplasmic viral inclusions were observed in duct cells of infected animals. Viral inclusion counts and infectivity titers in the salivary gland reached maximum levels by 3 to 4 weeks after infection; infectivity persisted, though at reduced levels, for at least 30 weeks. Electron microscopic examination of viral inclusions revealed several developmental events including nucleocapsid assembly, envelopment of nucleocapsids at the inner nuclear membrane and their enclosure by a thin vacuolar membrane. While contained within cytoplasmic vacuoles, enveloped virions acquired surface spikes. Cytoplasmic vacuoles containing virions subsequently coalesced and discharged mature virions at the cell surface into the lumen of the salivary gland duct. The data indicate that the ultrastructural development of GPCMV in the guinea pig salivary gland shows many similarities to that of human cytomegalovirus in humans. The salivary gland may provide a primary locus for virus shedding and horizontal transmission of cytomegalovirus.

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“…10). These filaments have been shown to contain viral DNA (10,12,20). Occasionally, the capsids revealed an electron dense, toroid structure which contained in its center a filamentous matrix of low electron density extending between two sides of the capsid wall (Fig.…”

Section: Replication M~turation and Exclusion Of The Progeny Virusmentioning

confidence: 99%

Ultrastructural studies on the replication of herpes virus ateles-73 in owl monkey kidney cells

Luetzeler

Heine

Wendel

et al. 1979

Archives of Virology

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The replicative cycle of herpesvirus ateles, strain 73 (HVA-73), was examined in the electron microscope and compared to that of other herpesviruses known to be oncogenic. A relatively slow replicative cycle of HVA-73 in owl monkey kidney (OMK) cells allowed us to distinguish cytoplasmic and nuclear stages of replication, comprising virus uptake, transport, maturation, and extrusion. Virus uptake was observed within 10 hours of infection and occurred both as a result of fusion between virus and cell membranes and by phagocytosis. Morphologic evidence for the transfer of viral DNA from nuclecapsids to the nucleus at the nuclear membrane is presented. This is shown by the location of numerous empty capsids in front of nuclear pores early during infection. Towards the end of the eclipse phase, at about 48 hougs after infection, two different types of nuclear inclusion bodies were observed. Progeny nucleocapsids were detected in the nucleus at the same time. The envelopment of nucleocapsids occurred both at the nuclear membrane and at proliferating Golgi lamellae in the cytoplasm. Each site of envelopment is associated with the maturation of a characteristic, morphologically distinguishable virus particle. The assembly of HVA-73 resembled that of other oncogenic herpesviruses.

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Nuclear Accumulation of Filamentous Herpes Simplex Virus DNA Late during the Replicative Cycle

Cited by 9 publications

References 9 publications

Replication of herpes-like viruses in haemocytes of adult flat oysters Ostrea angasi:an ultrastructural study

Replication of herpes-like viruses in haemocytes of adult flat oysters Ostrea angasi:an ultrastructural study

Ultrastructural development and persistence of guinea pig cytomegalovirus in duct cells of guinea pig submaxillary gland

Ultrastructural studies on the replication of herpes virus ateles-73 in owl monkey kidney cells

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