Nuclear alterations associated to programmed cell death in larval salivary glands of Apis mellifera (Hymenoptera: Apidae)

Silva-Zacarín, Elaine C.M.; Taboga, Sebastião Roberto; Moraes, Renata Mendonça

doi:10.1016/j.micron.2006.12.001

Cited by 30 publications

(16 citation statements)

References 46 publications

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“…Highly picnotic nuclei indicate low transcriptional activity due to chromatinic compacting, suggesting that the cells are in an advanced cell death process (Häcker 2000;Silva-Zacarin et al 2008). In midgut cells already in the lumen, for instance, nuclear picnosis indicates that these cells are already dead.…”

Section: Discussionmentioning

confidence: 99%

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

et al. 2009

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Morphological alterations, by means of histological and ultrastructural analysis, have been used to determine the effects of boric acid and fipronil on midgut tissues of honeybee worker, Apis mellifera L. larvae. In order to observe possible morphological alterations in the midgut, two groups of bioassays were performed. In the first one, the larvae were chronically treated with different concentrations of boric acid added to the food (1.0, 2.5 and 7.5 mg/g). In the second group, the larvae were fed with diets containing different concentrations of fipronil (0.1 and 1 microg/g) and compared with control groups without these chemical compounds. In the first bioassay, the larvae were collected on day 3 and in the second bioassay on day 4, when the mortality rate obtained in the toxicological bioassay was not very high. The larval midguts were removed and processed for morphological analyses using a light and transmission electron microscopy. We observed cytoplasmic vacuolizations, with the absence of autophagic vacuoles, and chromatinic compacting in most of the cells in the groups treated with pesticides. The morphological alterations were far greater in the larvae treated with boric acid than in the larvae treated with fipronil. Our data suggest that the midgut cell death observed was in response to boric acid and fipronil action. This study significantly improves the understanding of the toxicological effect of these insecticides from the ecotoxicological perspective.

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Section: Discussionmentioning

confidence: 99%

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

et al. 2009

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“…Programmed cell death (PCD) is extensively studied in humans (in the context of oncology), in vertebrates (mainly in mammals), and also in invertebrates like insects, both in vivo and in vitro (see Buss et al 2006), especially on metamorphosis and larval development (Bowen et al 1996;Silva-Zacarin et al 2006, 2007, and in nematodes. All these investigations are based on experiments that focus mostly on one kind of tissue.…”

Section: Discussionmentioning

confidence: 99%

Natural cyclic degeneration by a sequence of programmed cell death modes in Semibalanus balanoides (Linnaeus, 1767) (Crustacea, Cirripedia Thoracica)

2007

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The male reproductive system of the barnacle Semibalanus balanoides (Linnaeus, 1767) is a perfect model system for investigating naturally occurring cyclic nonpathological degeneration and programmed cell death (PCD). Every year after copulation, the barnacle degenerates its gonads, eggs, spermatids and penis initiated by severe environmental constraints. This is apparently a useful strategy for saving energy. By careful ultrastructural and immunohistochemical analysis of the male reproductive organs, we identiWed autophagic cell death in the penis and spermatids, both morphologically on ultrathin sections as well as histochemically for the Wrst time on semithin tissue sections with the new antibody against microtubule-associated protein 1 light chain 3 (LC3). Apoptosis in the testis and vesicula seminalis was determined based on the morphological characters on the apoptosis-speciWc antibody against the apoptosis inducing factor (AIF), and on positive TUNEL (terminal in situ nick end labeling) nuclei. Secondary necrosis in late degrading stages occurred in all tissues investigated, based on morphological characters and weak TUNEL staining. Moreover, the ultrastructural screening showed transient forms of cell death in the ductus ejaculatorius epithelium, the epidermis, and the longitudinal muscles of the penis. According to the immunohistochemical tests, the cyclic degeneration of the male reproductive system follows a sequence of programmed cell death modes from autophagic cell death via apoptosis to secondary necrosis.

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“…Epidermal cells of exocrine glands are class I with secretory features in newly emerged F. schrottkyi workers. However, large nuclei with decondensed chromatin in the spherical class III secretory cells suggest high activity (Silva-Zacarin et al, 2008).…”

Section: Discussionmentioning

confidence: 94%

Post-embryonic development of intramandibular glands of Friesella schrottkyi (Friese, 1900) (Hymenoptera: Apidae) workers

et al. 2017

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Nuclear alterations associated to programmed cell death in larval salivary glands of Apis mellifera (Hymenoptera: Apidae)

Cited by 30 publications

References 46 publications

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

Natural cyclic degeneration by a sequence of programmed cell death modes in Semibalanus balanoides (Linnaeus, 1767) (Crustacea, Cirripedia Thoracica)

Post-embryonic development of intramandibular glands of Friesella schrottkyi (Friese, 1900) (Hymenoptera: Apidae) workers

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