Nuclear and chromatin lipids: metabolism in normal and γ-irradiated rats

Kolomiytseva, I. K.; Кулагина, Т. П.; Markevich, L. N.; Archipov, V.I; Slozhenikina, L. V.; Fialkovskaya, L. A.; Potekhina, N.I.

doi:10.1016/s1567-5394(02)00126-3

Cited by 12 publications

(5 citation statements)

References 31 publications

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“…Biochemical changes including modification of the lipid composition in thymic cells are the basic morphological manifestations of radiation damage to the thymus (Kolomiytseva et al 2002).…”

Section: J U S T a C C E P T E Dmentioning

confidence: 99%

Modifying effects of low-intensity extremely high-frequency electromagnetic radiation on content and composition of fatty acids in thymus of mice exposed to X-rays

Gapeyev

Aripovsky

Кулагина

2015

International Journal of Radiation Biology

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“…Biochemical changes including modification of the lipid composition in thymic cells are the basic morphological manifestations of radiation damage to the thymus (Kolomiytseva et al 2002).…”

Section: J U S T a C C E P T E Dmentioning

confidence: 99%

Modifying effects of low-intensity extremely high-frequency electromagnetic radiation on content and composition of fatty acids in thymus of mice exposed to X-rays

Gapeyev

Aripovsky

Кулагина

2015

International Journal of Radiation Biology

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“…Ionizing radiation has enough energy to cause electronic excitation, i.e., ionization, producing the breakdown of chemical bonds in the biological molecules (DNA, lipids, proteins) of tissues 13,14 . Lipid peroxidation is a cytotoxic process defined by successive biochemical events triggered by free radicals that determine the extensive oxidation of unsaturated fatty acids and membranes.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular changes may be associated with the alteration and degeneration of myelin, present in numerous neurodegenerative diseases. It is still necessary to assess whether oxidative damage can lead to cell membrane dysfunction, causing changes in metabolism, selective change in cell permeability and signaling, which can lead to apoptosis 14,[16][17][18] .…”

Section: Discussionmentioning

confidence: 99%

Analytical methods for assessing changes induced by gamma exposure in an animal model

Mingote

Campos

Augusti

et al. 2020

Rev. Assoc. Med. Bras.

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SUMMARY OBJECTIVE: Ionizing radiation can cause radio-induced changes in the cellular metabolome due to the breakdown of DNA bonds. Our goal was to find the early tissue response to radiation exposure supported by distinct analytical methods. METHODS: Histological analyses were performed on the organs extracted from rats to search for microscopic changes. The histological slides stained with hematoxyline-eosin (HE) were analyzed in magnification (40x). Subsequently, the tissues were subjected to mass spectrometry that allowed molecular analysis and DESI-MSI that generated the molecular image of lipids, assessing changes in intensities, especially in the brain. RESULTS: The histological analysis found nonspecific inflammatory changes; no areas of fibrosis, necrosis, or apoptosis were identified, suggesting non-morphological tissue alterations. However, the DESI-MSI images of brain lipids allowed the observation of many radio-induced changes in the lipid's intensities. CONCLUSIONS: No early radio induced histological or mass weight changes in the radiation exposed rats could be observed at 5 Gy. However, early changes in the molecular level were observed in the DESI-MSI images of the brain lipids. The DESI-MSI method proved to be efficient and relevant, allowing a regional molecular analysis of the tissues, expanding a new field of study that is still in its infancy: radiometabolomics.

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“…Dose and dose rate are essential in the analysis of energy absorbed in RT patients. The clinical response to a deterministic effect is directly related to the frequency and dose rate absorbed when exceeding a dose threshold relative to the sensitivity of the tissue exposed to its effect [10].…”

Section: Discussionmentioning

confidence: 99%

“…From literature, data from brain tissue of guinea pigs suggest a cellular response to oxidative stress. Polyunsaturated fatty acids and cell membrane structural lipids are widely susceptible to gamma irradiation damage causing alteration of permeability, cell signaling, and apoptosis [10,12,13]. Lipid peroxidation is a cytotoxic event defined by successive biochemical events triggered by free radicals determining the extensive oxidation of unsaturated fatty acids and membranes.…”

Section: Discussionmentioning

confidence: 99%

METODOLOGICAL ISSUES IN PROTEIN AND LIPIDIC EXPRESSIONS IN BRAIN TISSUE EXPOSED TO Co60 BASED ON DESI/MALDI-MSI

Mingote¹

2019

Braz. J. Rad. Sci.

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The present paper attempts to present important issues in the methodology of identifying lipid and protein changes in brain tissue induced by radiation. The goal was to address the analysis of the methodology and to investigate the feasibility of the generation of lipid/protein profiles of irradiated brain tissue; disregarding the results of the analyses. Lipids and proteins are biomolecules with diverse structures and functionalities that participate in important intracellular processes. Changes in the lipid and the tissue protein profiles may indicate a cellular response to an external stimulus as well as the emergence of neoplasms or neurodegenerative diseases such as Alzheimer's. DESI-MSI is a convenient method for identifying lipids and their spatial distribution in tissue beyond analytical quantification. DESI-MS allows the creation of an image of several low lipid m/z classes. MALDI-MSI has already been a method used in the study of macromolecules as structural, membrane, hormone, neuromediator and immunological peptides. Through a full matrix scan, with a m/z spectrum between 500-1000 for lipids and with a mass spectrum of 1000-15000 Da for proteins, the molecular profiles can be analyzed generating 2D chemical pixel-type images. The produced image allows to associate the distribution of the lipids and proteins in the tissues with their chemical profile identified, allowing the verification of the changes radioinduced. Radiation triggers intense oxidative stress by increasing reactive oxygen species (ROS) and free radicals, causing DNA-damage consequently with alterations in proteomics including cellular lipid and protein expressions. The cellular morphophysiological changes are responsible for both the clonogenic inhibition and the induction of the apoptotic process. The images´s production was directly dependent on the rigorous execution of the methodological procedures. Innumerable interferences could impair the image´s generations and protein and lipid analysis. The main interferences in the present MS methodology were addressed and discussed. Following a well-established methodology, DESI can generate lipid and MALDI protein spectra in brain tissues. Near future, it will be possible to detect changes of the spatial distribution and the types of lipids and proteins in the white and gray matter regions.

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Nuclear and chromatin lipids: metabolism in normal and γ-irradiated rats

Cited by 12 publications

References 31 publications

Modifying effects of low-intensity extremely high-frequency electromagnetic radiation on content and composition of fatty acids in thymus of mice exposed to X-rays

Modifying effects of low-intensity extremely high-frequency electromagnetic radiation on content and composition of fatty acids in thymus of mice exposed to X-rays

Analytical methods for assessing changes induced by gamma exposure in an animal model

METODOLOGICAL ISSUES IN PROTEIN AND LIPIDIC EXPRESSIONS IN BRAIN TISSUE EXPOSED TO Co60 BASED ON DESI/MALDI-MSI

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