The nuclear electric quadrupole moment of 41Ca, which contains a single neutron outside closed proton and neutron shells (N=Z=20) has been determined. For this purpose the hyperfine structure (hfs) of the metastable 4s4p 3P 1 state was measured by a highly sensitive technique of combined radiofrequency laser saturation spectroscopy. Because of the small size of the quadrupole interaction constant B corrections for second order effects had to be included in the analysis. Based on corrected hfs constants for the 4s4p 3p~ state of the isotopes 41'43'45Ca and on a recent ab initio calculation of (r -3) integrals [1] one obtains in a consistent way: Q(41Ca)= -80(8) mb Q(43Ca)= -49(5) mb [1] Q(45Ca) = + 46(14) mb.These values include corrections for the electronic core polarization and relativistic effects. Moreover, the hfs anomaly in the 3p~ state was derived from the corrected Afactors and the nuclear magnetic moments of 41'43Ca. The value is 41A43= -1.16(39). 10 -4.