Nuclear-Cytoplasmatic Relations in the Cardiomyocytes and Endotheliocytes of the Pulmonary Heart Ventricles

Hnatiuk, M. S.; Slabyi, O. B.; Татарчук, Л. В.

doi:10.24061/1727-0847.15.1.2016.16

Cited by 5 publications

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“…According to present researchers, nuclearcytoplasmic relations are a valuable morphometric indicator that adequately reflects the peculiarities of cell function and metabolism, degree of disturbances of cellular structural homeostasis [1,6,16]. The nucleus and cytoplasm of cells are separated from each other, but at the same time they are closely interconnected and integrated and form a single structural and functional system.…”

Section: Discussionmentioning

confidence: 99%

“…The specified morphometric parameter decreases in case of maturation of cells and their differentiation that is caused to some extent by increase in cytoplasm and its hyperplasia of specific functioning organelles. The relationship between the spatial charac teristics of the nucleus and cytoplasm of the cell can also change with cell division, growth, diploidy [6,16].…”

Section: Discussionmentioning

confidence: 99%

“…Microtome sections 5-6 mcr thick after dewaxing were stained with hematoxylin-eosin, according to van Gizon, Mallory, Weigert, toluidine blue [7]. Histological micropreparations determined the height of spermatogenic cells, the diameter of their nuclei, nuclear-cytoplasmic ratios, the relative volume of damaged epitheliocytes (VODE) [1,6,16]. Morphometry of these structures was performed using a light microscope "Olimpus BX-2" with a digital video camera and application package "Video Test 5.0" and "Video size 5.0".…”

Section: Methodsmentioning

confidence: 99%

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Morphometric Evaluation of Spermatogenic Epitheliocytes Structure Under the Rubomycin Influence in Rats

Hnatiuk¹,

Коноваленко²,

Татарчук³

et al. 2020

IJMMR

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Background. Over the last 20 years, the quality of men’s sperm around the world has deteriorated twice. The causes are poor environment, stress, sedentary lifestyle, prevalence of sexually transmitted infections, obesity, alcohol abuse and various stimulants. The structure and function of the testes, when exposed to various drugs and chemicals, attracts the attention of present researchers. Objectives. The aim of the research was to study the structural changes of the spermatogenic epithelium in testicular damage caused by rubomycin hydrochloride. Methods. The spermatogenic epithelium of the testes of 30 white adult white male rats, which were divided into 2 groups, was morphometrically examined. The 1st group comprised 15 experimental intact animals, the 2nd – 15 rats, in which testicular damage with rubomycin hydrochloride was simulated. Euthanasia of rats was performed by bloodletting under thiopental anesthesia one month after the experiment beginning. Quantitative morphological parameters were statistically processed. In spermatocytes of the 1st P-order, spermatogonia and spermatids of testes, their height, diameter of nuclei, nuclear-cytoplasmic ratio and relative volumes of damaged cells of spermatogenic epithelium were evaluated. Results. It was established that spermatogenic epithelial cells were damaged in the simulated experimental conditions, which was morphometrically confirmed by changes in nuclear-cytoplasmic relations in the 1st, 2nd order spermatocytes, spermatogonia and spermatids. The relative volumes of damaged spermatogenic epithelial cells also increased significantly. The most significant morphometric parameters were altered in spermatids. Thus, the nuclear-cytoplasmic ratio in the studied cells was statistically significantly (p <0.001) increased by 7.4% compared with the similar control morphometric parameter. The relative volume of damaged spermatids in these experimental conditions reached (32.50±0.18) %, which with a high statistically significant difference (p<0.001) exceeded the same control value in almost 15.8 times. Optically, histological preparations of the testes showed severe vascular disorders, characterized by dilation and plethora mostly of venous vessels, which pointed to violation of venous drainage of the studied organs. Conclusions. According to the attained research results it has been established that nuclear-cytoplasmic relations are an objective and valuable informative indicator of a functional condition of cells and their structural changes in pathological conditions. When rubomycin hydrochloride is exposed to experimental animals, the most significant changes in nuclear-cytoplasmic relations are found in spermatic spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Morphometric Evaluation of Spermatogenic Epitheliocytes Structure Under the Rubomycin Influence in Rats

Hnatiuk¹,

Коноваленко²,

Татарчук³

et al. 2020

IJMMR

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“…Morphometrically in the left (LT) and right (RT) testicles measured the height of endothelial cells of arteries (HEA) and veins (HEV), the diameter of endothelial cells of arteries (DEA) and veins (DEV), nuclear-cytoplasmic ratios in these cells (NCRA, NCRV), as well as the relative volumes of damaged endothelial cells of arteries (RVDEA) and veins (RVDEV) [4,5].…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, morphologists are increasingly using morphometric research methods that allow to quantify and most objectively assess various physiological and pathological processes in organs and systems and logically interpret them [3,4,17].…”

Section: Introductionmentioning

confidence: 99%

Quantitative morphological evaluation of structural reconstruction in endothelial cells of arteries and veins of testes in the conditions at postresection pulmonary arterial hypertension

Hnatjuk

Коноваленко

Татарчук

et al. 2019

Biomed. and Biosoc. Anthrop.

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Removal of the right or left lung can lead to postresection pulmonary arterial hypertension, venous stasis in the organs of the great circle of blood circulation and structural and functional changes in them. Morphological reconstruction of endotheliocytes of arteries and veins of testes at postresection pulmonary arterial hypertension are insufficiently studied. The purpose of our study was to conduct a quantitative morphological evaluation of structural changes of endothelial cells of the arteries and veins of the testes at postresection pulmonary arterial hypertension. The testes of 46 white male rats, which were divided into 3 groups, were morphological examined. The 1st group consisted of 14 intact animals, 2st – 22 rats with postresection pulmonary arterial hypertension and compensated cor pulmonale, 3st – 10 animals with postresection pulmonary hypertension and decompensated cor pulmonale. Postresection pulmonary arterial hypertension and cor pulmonale were modeled by performing right pulmonectomy in rats. Euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia one month after the start of the experiment. From the testes were made micropreparations, which morphometrically determined the height of endothelial cells of arteries and veins, the diameter of their nuclei, nuclear-cytoplasmic ratios in these cells, the relative volumes of damaged endotheliocytes. Quantitative indicators were processed statistically. In the conditions of postresection pulmonary arterial hypertension and decompensated pulmonary heart, the studied morphometric parameters changed most markedly. The height of endothelial cells of the arteries of the left testis was reduced by 2.9 % (p˂0.05), and the right – by 2.3 %. Nuclear-cytoplasmic ratios in the endotheliocytes of these vessels increased by 7.6 % and 4.7 %, respectively (p˂0.05), and the relative volume of damaged endotheliocytes increased by 11.7 and 9.3 times (p˂0.001). The studied morphometric parameters in the testicular veins increased to a greater extent compared with the arteries. Thus, the height of endothelial cells of the left testis in the 3rd group of observations was statistically significant (p˂0.001) decreased by 5.8 %, and in the right – by 5.4 %. Nuclear-cytoplasmic ratios in the studied structures increased by 8.2 % (p˂0.001) and 6.5 % (p˂0.01), respectively, which indicated a pronounced violation of structural cellular homeostasis. The relative volume of damaged endothelial cells in the veins of the left testis in postresection pulmonary arterial hypertension combined with decompensated heart increased by 17.6, in the right – by 13.0 times (p˂0.001). Optical on histological preparations of testes showed plethora and dilation of venous vessels, which was complicated by hypoxia, dystrophy, necrobiosis of cells and tissues of the studied organ, and in the long term – infiltrative and sclerotic processes. Venous vessels of the microhemocirculatory bed are unevenly dilated, tortuous, full-blooded, with numerous varicose veins and sacculations. Stasis, thrombosis, diapedetic hemorrhages, plasmorrhagia of the walls of venous vessels and paravasal tissues were found in these vessels. Elastic structures in vessels with the phenomena of multiplication, fragmentation and destruction. Endotheliocytes with signs of edema, dystrophically and necrobiotically altered, sometimes desquamated. Analysis of the obtained data revealed that postresection pulmonary arterial hypertension leads to violations of cellular structural homeostasis, an increase in the relative volumes of damaged endotheliocytes in the vascular bed of the testes. The revealed processes dominate in the venous bed of the left testis and in the decompensated cor pulmonale.

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Кількісний Морфологічний Аналіз Особливостей Ремоделювання Венозного Русла Нижньої Кінцівки При Експериментальних Ранах

Hnatjuk¹,

Кріцак²,

Абдулела³

2023

ЗКЕМ

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РЕЗЮМЕ. Мета – дослідити особливості ремоделювання венозного русла нижньої кінцівки при перебігу експериментальних ран в умовах венозної гіпертензії. Матеріал і методи. Досліджено венозне русло нижньої кінцівки 45 білих щурів, які були поділені на 3 три групи. 1 група контрольна – 15 інтактних тварин, 2 – 15 щурів із 10-денною змодельованою раною задньої кінцівки та венозною гіпертензією, 3 – 15 тварин з аналогічною 14-денною раною. Евтаназію тварин здійснювали кровопусканням в умовах тіопенталового наркозу. На мікропрепаратах задньої кінцівки визначали зовнішній та внутрішній діаметри венозних судин, товщину їх стінки, висоту ендотеліоцитів, діаметр їх ядер, ядерно-цитоплазматичні відношення у цих клітинах, відносний об’єм ушкоджених ендотеліоцитів. Кількісні величини обробляли статистично. Результати. Встановлено, що змодельована патологія призводила до вираженої структурної перебудови вен нижньої кінцівки експериментальних тварин. При змодельованій патології відбувалося збільшення зовнішнього та внутрішнього діаметрів вен, зменшення товщини їх стінок, порушення клітинного структурного гомеостазу в ендотеліоцитах, зростання відносних об'ємів пошкоджених ендотеліоцитів, повнокров'я венозних судин, порушення венозного дренажу, дистрофічні, некробіотичні, інфільтративні та склеротичні процеси у досліджуваній кінцівці, які домінували на 14 день експерименту.

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Nuclear-Cytoplasmatic Relations in the Cardiomyocytes and Endotheliocytes of the Pulmonary Heart Ventricles

Cited by 5 publications

References 0 publications

Morphometric Evaluation of Spermatogenic Epitheliocytes Structure Under the Rubomycin Influence in Rats

Morphometric Evaluation of Spermatogenic Epitheliocytes Structure Under the Rubomycin Influence in Rats

Quantitative morphological evaluation of structural reconstruction in endothelial cells of arteries and veins of testes in the conditions at postresection pulmonary arterial hypertension

Кількісний Морфологічний Аналіз Особливостей Ремоделювання Венозного Русла Нижньої Кінцівки При Експериментальних Ранах

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