The High Temperature Engineering Test Reactor (HTTR) is a block type fuel High Temperature Gas-cooled Reactor (HTGR) constructed in Japan, firstly. The operating data of the HTTR with burn-up is very important for developments of HTGRs. Many test data have been collected in the HTTR. Many tests are carried out in low power operation. On the other hand, the full power operation is not enough. There is a temperature distribution in a core in full power operation. The temperature distribution in a core makes it difficult to validate the calculation code. Additionally, it is difficult to measure core temperature in HTTR. On the other hands, the data of the control rod position at criticality at zero power have been measured at the beginning of each operation cycle and the temperature distribution in a core at zero power is uniform. Therefore, the data at zero power are suitable for confirm the characteristics of burn-up and validation of calculation code. In this study, the calculated control rod positions at zero power criticality with burn-up are compared with the experimental data with correlation of core temperature. The calculated results of criticality control rod position at zero power show good agreement to the experimental data. It means that calculated result shows appropriate decrease in uranium and accumulation in plutonium decrease in burnable absorber with burn-up.