Nuclear DNA content and karyotype of Rosewood (Aniba rosaeodora)

Contim, Luis Antônio Serrão; Carvalho, Carlos Roberto; Martins, Francielle Alline; Freitas, Danival Vieira de

doi:10.1590/s1415-47572005000500017

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…These techniques allowed the obtention of chromosomes well-spread in the same focus plane, without cytoplasmic background, overlaps or structural deformations. Identical results were obtained in Zea mays Saraiva 1993, 1997), Capsicum annuum (Almeida and Carvalho 2004) and Aniba rosaeodora (Contim et al 2005).…”

Section: Discussionsupporting

confidence: 68%

Classical and Molecular Cytogenetic Tools to Resolve the Bixa Karyotypes

Almeida

Carvalho

2006

CYTOLOGIA

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SummaryThe cytogenetic studies performed in Bixa orellana have reported 2nϭ14 or 2nϭ16 chromosomes with little karyotypic information. In this context, an improved cytogenetic protocol using enzymatic maceration of meristematic cellular walls and digital image analysis was applied to B. orellana and B. arborea. In B. arborea, the high-resolution technique, Ag-NOR and C banding methods, and FISH using a probe of 45S rDNA genes, were also performed. Prometaphasic and standard C-metaphasic chromosomes presented well-defined primary and secondary constrictions facilitated the pairing of homologues and assembly of the karyogram for the 2 species. These species showed similar karyotypes with 2nϭ14 chromosomes, being composed of 5 metacentric pairs (1, 2, 3, 4 and 6) and 2 submetacentric pairs (5 and 7). The chromosome 1 is approximately 2 times as long as the others and possesses the secondary constriction adjacent to the centromere, which suggests that Robertsonian translocation between 2 smaller chromosomes may have occurred during the karyotype evolution of Bixa. Additionally, the active NOR, the NOR-adjacent heterochromatic region and 18S, 5.8S and 26S rDNA genes were identified in the secondary constriction of chromosome 1.

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Section: Discussionsupporting

confidence: 68%

Classical and Molecular Cytogenetic Tools to Resolve the Bixa Karyotypes

Almeida

Carvalho

2006

CYTOLOGIA

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“…The Amazon region represents one of the richest reservoirs of biological diversity on the planet (Confalonieri et al 2014;Gentry 1992), and is considered a biodiversity hotspot that can serve as a potential source of genetic variability for breeding perspectives of crops (Myers et al 2000;Gentry 1992). Rosewood, Aniba rosaeodora Ducke (Lauraceae) has 2n = 24 chromosomes (Contim et al 2005), and is distributed in the Amazon region of Brazil, Guyana, Suriname, Peru, Colombia, and Venezuela (Maia and Mourão 2016). The species is known for its essential oil, which is mainly characterized by a high content of linalool in the leaves and branches (74.4 -81.8%) (Pimentel et al 2018) and in the trunk wood (~ 100%) (Chantraine et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers

et al. 2020

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Rosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed the presence of maximum variability within populations (88%). The Structure algorithm, neighbor joining and principal coordinate analysis (PCoA) grouped the 90 rosewood accessions into three main populations (A, B and C). Diversity indices at the inter-population level revealed a greater genetic diversity in population A, due to higher gene flow. The neighbor-joining analysis grouped populations A and B, while population C was found to be divergent at the inter population level. We concluded that population A reflects higher genetic diversity and should be prioritized for future management and conservation plans.

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“…In view of this similarity, both DAPI and PI can be used for flow cytometry analysis of H. salsaparilha. Concurrently, Contim et al (2005) reported similar responses for Rosewood (Aniba rosaeodora). The 2C value of rosewood showed a nuclear DNA content of 2.36 pg using DAPI or 2.32 pg using PI as fluorochromes.…”

Section: Flow Cytometry Analysis and Dna Quantificationmentioning

confidence: 70%

Cytogenetics and Flow Cytometry-based DNA Quantification in Herreria salsaparilha Martius (Herreriaceae): a Medicinal Species

et al. 2007

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Adventitious root tips developing from base of cuttings and leaf samples of Herreria salsaparilha were used to determine the karyotype and to estimate DNA amount, respectively. Flow cytometry methodology was used with DAPI (4Ј,6-diamidino-2-phenylindole) and PI (propidium iodide) as specific DNA fluorochromes, and leaf samples of Pisum sativum (9.09 pg) as an internal standard. Cytogenetic techniques determined that H. salsaparilha has 29 pairs of homologous chromosomes (2nϭ58), 12 acrocentric pairs (length varying from 1.75 to 10.51 mm), 16 submetacentric pairs (1.30 to 3.45 mm), and 1 metacentric pair (2.8 mm). The DAPI-and PI-based flow cytometry analysis revealed that H. salsaparilha has nuclear DNA content 2Cϭ5.5 pg, equivalent to approximately 5.31ϫ10 9 base pairs. This karyotype asymmetric type may suggest an evolutionary process with involvement of polyploidization, being justified by the relative increased chromosome number (2nϭ58) and fusions.

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Nuclear DNA content and karyotype of Rosewood (Aniba rosaeodora)

Cited by 10 publications

References 14 publications

Classical and Molecular Cytogenetic Tools to Resolve the Bixa Karyotypes

Classical and Molecular Cytogenetic Tools to Resolve the Bixa Karyotypes

Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers

Cytogenetics and Flow Cytometry-based DNA Quantification in Herreria salsaparilha Martius (Herreriaceae): a Medicinal Species

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