Nuclear DNA content in the genus Hepatica (Ranunculaceae)

Mabuchi, Tomoo; Kokubun, Hisashi; Mii, Masahiro; Ando, Toshio

doi:10.1007/s10265-005-0191-9

Cited by 15 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pubescens (m.Hiroe) Kitam. belong to the entire leaf group (Mabuchi et al ., ). Phylogenetic studies to date and this study have failed to resolve infrageneric relationships in Hepatica (Weiss‐Schneeweiss et al ., ; Pfosser et al ., ).…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Diverse evolutionary pathways shaped 5S rDNA of species of tribe Anemoneae (Ranunculaceae) and reveal phylogenetic signal

et al. 2016

View full text Add to dashboard Cite

Anemone sensu lato (including Pulsatilla and Hepatica), tribe Anemoneae (Ranunculaceae), is arranged into two subgenera, Anemone and Anemonidium, with basic chromosome numbers x = 8 and x = 7, respectively. We elucidated the level of divergence of 5S rDNA unit arrays between the subgenera, determined intra‐individual and interspecific sequence variation and tested 5S rDNA phylogenetic signal in revealing the origin of polyploid species. High intra‐individual nucleotide diversity and the presence of 5S rDNA unit array length variants and pseudogenes indicate that weak homogenization forces have shaped 5S rDNA in the investigated species. Our results show that 5S rDNA evolved through two major changes: diversification of 5S rDNA into two lineages, one with long (subgenus Anemone) and one with short 5S rDNA unit arrays (subgenus Anemonidium); and subsequent contraction and expansion of 5S rDNA unit arrays. Phylogenetic analysis based on 5S rDNA supports the hypothesis that A. parviflora could be a parental species and donor of the subgenome D to the allopolyploids A. multifida (BBDD) and A. baldensis (AABBDD). In A. baldensis interlocus exchange possibly occurred, followed by subsequent replacement of the 5S rDNA from subgenome D with those from subgenome B. Here we present evidence that both models, concerted and birth‐and‐death evolution, were probably involved in the evolution of the 5S rDNA multigene family in subgenera Anemone and Anemonidium.

show abstract

Section: Discussionmentioning

confidence: 97%

“…Hepatica, a small genus of about a dozen species distributed in the temperate zones of the Northern Hemisphere, is classified into two groups according to leaf morphology (Mabuchi et al, 2005). The crenate leaf group comprised mostly polyploid species and the only diploid H. falconeri Steward, whereas the remaining diploids and polyploid H. nobilis var.…”

mentioning

confidence: 99%

Diverse evolutionary pathways shaped 5S rDNA of species of tribe Anemoneae (Ranunculaceae) and reveal phylogenetic signal

et al. 2016

View full text Add to dashboard Cite

show abstract

“…These are, however, clearly outliers as they are nearly twice the size of the next largest eudicot genome in the genus Hepatica (H. nobilis var. pubescens; 2n = 4x = 28; 1C = 44.6 pg) in Ranunculaceae [61]. As Figure 3(c) shows, even this is an outlier as 99.5% of all eudicots have genomes smaller than 25 pg.…”

Section: Genome Size Diversity Across Angiospermsmentioning

confidence: 87%

Genome Size Dynamics and Evolution in Monocots

et al. 2010

View full text Add to dashboard Cite

Monocot genomic diversity includes striking variation at many levels. This paper compares various genomic characters (e.g., range of chromosome numbers and ploidy levels, occurrence of endopolyploidy, GC content, chromosome packaging and organization, genome size) between monocots and the remaining angiosperms to discern just how distinctive monocot genomes are. One of the most notable features of monocots is their wide range and diversity of genome sizes, including the species with the largest genome so far reported in plants. This genomic character is analysed in greater detail, within a phylogenetic context. By surveying available genome size and chromosome data it is apparent that different monocot orders follow distinctive modes of genome size and chromosome evolution. Further insights into genome size-evolution and dynamics were obtained using statistical modelling approaches to reconstruct the ancestral genome size at key nodes across the monocot phylogenetic tree. Such approaches reveal that while the ancestral genome size of all monocots was small (1C = 1.9 pg), there have been several major increases and decreases during monocot evolution. In addition, notable increases in the rates of genome size-evolution were found in Asparagales and Poales compared with other monocot lineages.

show abstract

“…However, karyotypically, H. falconeri is identical to the other diploid taxa (Ogisu et al ., 2002; this study), rendering any inferences from this character impossible. Genome size data (Mabuchi et al ., 2005), however, seem rather to support the hypothesis of allopolyploid origin. The nuclear DNA amount determined for H. henryi , H. transsilvanica and H. yamatutai agrees well with an essentially additive pattern of parental genome size, whereas the alternative hypothesis of autopolyploid origin requires additional and independent increase of genome size after autopolyploidization.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal stasis in diploids contrasts with genome restructuring in auto‐ and allopolyploid taxa ofHepatica(Ranunculaceae)

et al. 2007

Self Cite

View full text Add to dashboard Cite

Summary• Polyploidization and chromosomal rearrangements are recognized as major forces in plant evolution. Their role is investigated in the disjunctly distributed northern hemisphere Hepatica (Ranunculaceae).• Chromosome numbers, karyotype morphology, banding patterns, 5S and 35S rDNA localization in all known species were investigated and interpreted in a phylogenetic context established from nuclear internal transcribed spacer (ITS) and plastid matK sequences.• All species had a chromosome base number of x = 7. The karyotype was symmetric and showed little variation among diploids with one locus each of 5S and 35S rDNA, except for interpopulational variation concerning 35S rDNA loci number and localization in H. asiatica. Tetraploids exhibited chromosomal changes, including asymmetry and/or loss of rDNA loci. Nuclear and plastid sequences resulted in incongruent topologies because of the positions of some tetraploid taxa. The diversification of Hepatica occurred not earlier than the Pliocene.• Genome restructuring, especially involving 35S rDNA, within a few million yr or less characterizes evolution of both auto-and allopolyploids and of the diploid species H. asiatica, which is the presumptive ancestor of two other diploid species.

show abstract

Nuclear DNA content in the genus Hepatica (Ranunculaceae)

Cited by 15 publications

References 18 publications

Diverse evolutionary pathways shaped 5S rDNA of species of tribe Anemoneae (Ranunculaceae) and reveal phylogenetic signal

Diverse evolutionary pathways shaped 5S rDNA of species of tribe Anemoneae (Ranunculaceae) and reveal phylogenetic signal

Genome Size Dynamics and Evolution in Monocots

Chromosomal stasis in diploids contrasts with genome restructuring in auto‐ and allopolyploid taxa ofHepatica(Ranunculaceae)

Contact Info

Product

Resources

About