Nuclear DNA methylase from <i>Pisum sativum</i>

Yesufu, H. M. I.; Kuo, Yien–Ming; Adams, Roger

doi:10.1042/bst0160757

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzyme was assayed as previously described for the mouse DNA methylase except that the reaction was carried out at pH 7.2 and at 30°C for 2 h (Turnbull & Adams, 1976;Yesufu et al, 1988). One unit of activity is the amount that will catalyse the incorporation of 1 pmol (l04 d.p.m.)…”

Section: Dna Methylase Assaymentioning

confidence: 99%

See 1 more Smart Citation

DNA methylase from Pisum sativum

Yesufu

Hanley

Rinaldi

et al. 1991

Biochemical Journal

View full text Add to dashboard Cite

DNA methylase activity was detected in nuclei from pea shoots. The enzyme can only be extracted by low-salt treatment if the nuclei are pretreated with micrococcal nuclease. Only a single enzyme was detected, and it was purified to a specific activity of 1620 units/mg of protein. It has an Mr of 160,000 on gel filtration and SDS/PAGE. Pea DNA methylase methylates cytosine in all four dinucleotides, and this is interpreted to show that it acts on CNG trinucleotides. Although it shows a strong preference for hemi-methylated double-stranded DNA, it is also capable of methylation de novo. Homologous DNA is the best natural substrate. In vitro the enzyme interacts with DNA to form a salt-resistant complex with DNA that is stable for at least 4 h.

show abstract

Section: Dna Methylase Assaymentioning

confidence: 99%

“…Only one enzyme activity was detected, and the Mr of the native enzyme is 160000. A preliminary report of this work has already appeared (Yesufu et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

DNA methylase from Pisum sativum

Yesufu

Hanley

Rinaldi

et al. 1991

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…The situation is very similar in peas despite the broader range of methylation in plants. There appears to be only one enzyme, which has a native molecular mass of about 160000 (figure 2) and is located in the nucleus (Yesufu et al 1988(Yesufu et al , 1989.…”

Section: (B) Pea Dna Methylasementioning

confidence: 99%

Eukaryotic DNA methylases and their use forin vitromethylation

Adams

Bryans

Rinaldi

et al. 1990

Phil. Trans. R. Soc. Lond. B

View full text Add to dashboard Cite

DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclusively and show similar kinetics of methylation, which makes it difficult to saturate all possible sites on the DNA, but procedures are described that circumvent this problem.

show abstract

Nuclear DNA methylase from Pisum sativum

Cited by 2 publications

References 0 publications

DNA methylase from Pisum sativum

DNA methylase from Pisum sativum

Eukaryotic DNA methylases and their use forin vitromethylation

Contact Info

Product

Resources

About