Nuclear export of mRNA and its regulation by ubiquitylation

Durairaj, Geetha; Garg, P.; Bhaumik, Sukesh R.

doi:10.4161/rna.6.5.10078

Cited by 19 publications

(7 citation statements)

References 74 publications

(121 reference statements)

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“…Dramatic progress has been made in recent years in understanding the rich variety of mechanisms involved in the post‐transcriptional regulation of gene expression. Steady‐state levels of RNA transcript concentrations can be controlled by the regulation of nuclear RNA processing by the spliceosome complex (Rino & Carmo‐Fonseca, 2009), nuclear RNA degradation by the exosome complex (Belostotsky & Sieburth, 2009), mRNA nuclear export (Durairaj et al. , 2009), riboswitches (Breaker, 2008) and cytoplasmic degradation of transcript through miRNA binding and recruitment of the RNA‐induced silencing complex (RISC) (Kawamata & Tomari, 2010).…”

Section: Discussionmentioning

confidence: 99%

Evidence of function for conserved noncoding sequences in Arabidopsis thaliana

et al. 2011

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Summary• Whole genome duplication events provide a lineage with a large reservoir of genes that can be molded by evolutionary forces into phenotypes that fit alternative environments. A well-studied whole genome duplication, the a-event, occurred in an ancestor of the model plant Arabidopsis thaliana. Retained segments of the a-event have been defined in recent years in the form of duplicate protein coding sequences (a-pairs) and associated conserved noncoding DNA sequences (CNSs). Our aim was to identify any association between CNSs and a-pair co-functionality at the gene expression level.• Here, we tested for correlation between CNS counts and a-pair co-expression and expression intensity across nine expression datasets: aerial tissue, flowers, leaves, roots, rosettes, seedlings, seeds, shoots and whole plants.• We provide evidence for a putative regulatory role of the CNSs. The association of CNSs with a-pair co-expression and expression intensity varied by gene function, subgene position and the presence of transcription factor binding motifs. A range of possible CNS regulatory mechanisms, including intron-mediated enhancement, messenger RNA fold stability and transcriptional regulation, are discussed.• This study provides a framework to understand how CNS motifs are involved in the maintenance of gene expression after a whole genome duplication event.

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Section: Discussionmentioning

confidence: 99%

Evidence of function for conserved noncoding sequences in Arabidopsis thaliana

et al. 2011

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“…Durairaj et al proved that the HECT ubiquitin ligases as well as the cullin-RING ligase CSF Mdm30 are connected with the transport of mRNA from the nucleus to the cytoplasm. 29 Based on our data, we suspect that increased desmin mRNA expression may be considered as an important feature of early and late phases of compensatory mechanisms against worsening HF. The possibility of regulatory influence of desmin mRNA expression in the human heart by beta-blockers, immunoadsorption and subsequent immunoglobulin substitution creates a new therapeutic option for patients with IDCM.…”

Section: Patterns Of Desmin Mrna Expressionmentioning

confidence: 67%

Patterns of Desmin Expression in Idiopathic Dilated Cardiomyopathy are Related to the Desmin mRNA and Ubiquitin Expression

Pawlak

Rejmak

Gil

et al. 2019

Journal of Investigative Medicine

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Desmin expression depends on desmin messenger RNA (mRNA) and ubiquitin proteasome system. This process is poorly understood in dilated cardiomyopathy. The aim of the study was to investigate whether changes of desmin mRNA and ubiquitin expression correlate with types of desmin expression in cardiomyocytes. Endomyocardial biopsy was performed in 60 patients (85% men, mean age 46±14 years) with heart failure (HF; left ventricular ejection fraction <45%). Desmin and ubiquitin expression were analysed in histological sections by immunohistochemistry and in Western blot. Desmin mRNA expression was determined by fluorescent in situ hybridization methods. In patients with weak/even desmin expression, weak/even expression of ubiquitin in the cytosol and low desmin mRNA expression in the cytosol and nuclei of cardiomyocytes were observed. Expression of ubiquitin and desmin mRNA increased along with the progression of desmin cytoskeleton remodeling. Desmin mRNA and ubiquitin were weakly expressed/absent in cardiomyocytes with low/lack of desmin expression. Variations in desmin mRNA, desmin and ubiquitin expression were associated with gradual changes in myocardial structure and clinical parameters. To conclude, changes in ubiquitin and desmin mRNA expression are related to patterns of desmin expression. An increase in the expression of ubiquitin and desmin mRNA may be a protective feature against unfavorable cell remodeling. This may reduce the adverse effects of cytoskeleton damage in the early stages of HF. Low/lack ubiquitin and/or desmin mRNA expression may be markers of end-stage HF.

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“…Fine-tuning of the localisation/transport of a single protein or group of proteins, however, requires more specific modification, generally of the protein cargo itself rather than of the transport machinery. The best understood mechanism of regulating nuclear transport is through phosphorylation near the NLS/NES modifying recognition by IMP/EXP [37,38], but modifications such as acetylation, ubiquitinylation and sumoylation have also been described [39][40][41] to regulate nucleocytoplasmic trafficking of cellular proteins such as the tumour suppressors p110 Rb and p53, Survivin, nuclear factor NF-κB, the phosphatase PTEN and the NF-κB essential modulator NEMO [42][43][44][45][46][47][48]. It is significant in this context that viral proteins are often highly posttranslationally modified (see Table 1), including through the action of cyclin-dependent kinases (Cdks), which can serve to effect cell cycle-dependent modulation of nucleocytoplasmic trafficking.…”

Section: Regulation Of Nuclear Transportmentioning

confidence: 99%

Regulation of nucleocytoplasmic trafficking of viral proteins: An integral role in pathogenesis?

Fulcher

Jans

2011

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Signal-dependent targeting of proteins into and out of the nucleus is mediated by members of the importin (IMP) family of transport receptors, which recognise targeting signals within a cargo protein and mediate passage through the nuclear envelope-embedded nuclear pore complexes. Regulation of this process is paramount to processes such as cell division and differentiation, but is also critically important for viral replication and pathogenesis; phosphorylation appears to play a major role in regulating viral protein nucleocytoplasmic trafficking, along with other posttranslational modifications. This review focuses on viral proteins that utilise the host cell IMP machinery in order to traffic into/out of the nucleus, and in particular those where trafficking is critical to viral replication and/or pathogenesis, such as simian virus SV40 large tumour antigen (T-ag), human papilloma virus E1 protein, human cytomegalovirus processivity factor ppUL44, and various gene products from RNA viruses such as Rabies. Understanding of the mechanisms regulating viral protein nucleocytoplasmic trafficking is paramount to the future development of urgently needed specific and effective anti-viral therapeutics. This article was originally intended for the special issue "Regulation of Signaling and Cellular Fate through Modulation of Nuclear Protein Import". The Publisher apologizes for any inconvenience caused.

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Nuclear export of mRNA and its regulation by ubiquitylation

Cited by 19 publications

References 74 publications

Evidence of function for conserved noncoding sequences in Arabidopsis thaliana

Evidence of function for conserved noncoding sequences in Arabidopsis thaliana

Patterns of Desmin Expression in Idiopathic Dilated Cardiomyopathy are Related to the Desmin mRNA and Ubiquitin Expression

Regulation of nucleocytoplasmic trafficking of viral proteins: An integral role in pathogenesis?

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