Nuclear expression of claudin‑3 in human colorectal adenocarcinoma cell lines and tissues

Tokuhara, Yasunori; Morinishi, Tatsuya; Matsunaga, Toru; Sakai, Manabu; Sakai, Takayoshi; Ohsaki, Hiroyuki; Kadota, Kyuichi; Kushida, Yoshio; Haba, Reiji; Hirakawa, Eiichiro

doi:10.3892/ol.2017.7281

Cited by 19 publications

(17 citation statements)

References 63 publications

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“…Its delocalization into the nucleus was also observed in human osteosarcoma cells [81] and follicular thyroid carcinoma cells [82]. Moreover, claudin-2 was observed in the nucleus in human lung adenocarcinoma cells [83], and claudin-3 was reported to be present in nuclei in breast cancer cell lines [84] and human colorectal cancer [85]. Changes in claudin-4 location in endometrial cancer cells were also observed [86].…”

Section: Localization Of Claudins In Cellsmentioning

confidence: 74%

Claudins: New Players in Human Fertility and Reproductive System Cancers

Kozieł

Kowalska

Piastowska-Ciesielska

2020

Cancers

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Claudins are major integral proteins of tight junctions (TJs), the apical cell-cell adhesions that enable maintaining polarity of epithelial cells, their differentiation, and cell signaling. A number of studies have indicated that claudins might play a crucial role in both physiology and pathogenesis. Their tissue-specific expression was originally linked to the development of different types of cancer and triggered a hope to use them as diagnostic or prognostic markers. However, it seems that their expression is more complex than that, and undoubtedly, claudins participate in one of the most important molecular events in cells. This review summarizes the recent research evaluating the role of claudins in fertility and the most common endocrine-dependent cancers in the reproductive system and highlights the crucial role of claudins both in human fertility and the most common cancers.

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Section: Localization Of Claudins In Cellsmentioning

confidence: 74%

Claudins: New Players in Human Fertility and Reproductive System Cancers

Kozieł

Kowalska

Piastowska-Ciesielska

2020

Cancers

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“…Claudin family proteins consisting of at least 24 members are essential for the formation of TJs and have a significant effect on the biological behavior of tumor progression. Previous studies have demonstrated the several claudin (claudin-1 [62, 63], claudin-3 [64, 65], claudin-4 [64], claudin-7 [65]) aberrant expression patterns in colorectal cancer. Among them, claudin-1 and claudin-2 overexpression are identified in the present dataset.…”

Section: Resultsmentioning

confidence: 99%

FoPA: identifying perturbed signaling pathways in clinical conditions using formal methods

2019

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Background Accurate identification of perturbed signaling pathways based on differentially expressed genes between sample groups is one of the key factors in the understanding of diseases and druggable targets. Most pathway analysis methods prioritize impacted signaling pathways by incorporating pathway topology using simple graph-based models. Despite their relative success, these models are limited in describing all types of dependencies and interactions that exist in biological pathways. Results In this work, we propose a new approach based on the formal modeling of signaling pathways. Signaling pathways are formally modeled, and then model checking tools are applied to find the likelihood of perturbation for each pathway in a given condition. By adopting formal methods, various complex interactions among biological parts are modeled, which can contribute to reducing the false-positive rate of the proposed approach. We have developed a tool named Formal model checking based pathway analysis (FoPA) based on this approach. FoPA is compared with three well-known pathway analysis methods: PADOG, CePa, and SPIA on the benchmark of 36 GEO datasets from various diseases by applying the target pathway technique. This validation technique eliminates the need for possibly biased human assessments of results. In the cases that, there is no apriori knowledge of all relevant pathways, simulated false inputs (permuted class labels and decoy pathways) are chosen as a set of negative controls to test the false positive rate of the methods. Finally, to further evaluate the efficiency of FoPA, it is applied to a list of autism-related genes. Conclusions The results obtained by the target pathway technique demonstrate that FoPA is able to prioritize target pathways as well as PADOG but better than CePa and SPIA. Also, the false-positive rate of finding significant pathways using FoPA is lower than other compared methods. Also, FoPA can detect more consistent relevant pathways than other methods. The results of FoPA on autism-related genes highlight the role of “Renin-angiotensin system” pathway. This pathway has been supposed to have a pivotal role in some neurodegenerative diseases, while little attention has been paid to its impact on autism development so far. Electronic supplementary material The online version of this article (10.1186/s12859-019-2635-6) contains supplementary material, which is available to authorized users.

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“…Tokuhara и соавт . [14] [13] . Представленные в литературе данные позволяют высказать предположение о том, что ядерная экспрессия клаудина-3 может быть ассоциирована со степенью злокачественности и метастатическим потенциалом опухоли .…”

Section: Fig 3 Abnormal Nuclear Claudin-3 Expression In Gastric Canunclassified

Claudin-3 expression in gastric cancer

Данилова¹,

Anikina²,

Oleynikova³

et al. 2020

Arkh. patol.

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Клаудины являются одними из основных белков плотных контактов эпителиальных клеток. Изменение уровня экспрессии клаудинов может приводить к нарушению функции плотных контактов, что играет важную роль в прогрессии злокачественных новообразований. Экспрессия клаудина-3 в раке желудка изучена недостаточно. Цель исследования-изучить экспрессию клаудина-3 в раке желудка и в прилежащей нормальной слизистой оболочке и оценить взаимосвязь уровня экспрессии с основными клинико-морфологическими характеристиками опухоли. Материал и методы. В исследование включены образцы биопсийного и операционного материала от 69 пациентов с раком желудка. Для постановки иммуногистохимических реакций использовали поликлональные мышиные антитела к клаудину-3. Результаты. Экспрессия клаудина-3 в раке желудка была статистически значимо выше, чем в прилежащей нормальной слизистой оболочке (p<0,05). Отсутствие экспрессии клаудина-3 в раке желудка значимо ассоциировано с низкой степенью дифференцировки опухоли (p<0,05). В 69,6% случаев в раке желудка наблюдалась ядерная экспрессия клаудина-3, которая была ассоциирована с отсутствием мембранной экспрессии маркера (p<0,05). Заключение. Впервые описана аномальная ядерная экспрессия клаудина-3 в раке желудка и установлена значимая ассоциация между наличием ядерной экспрессии и отсутствием типичной мембранной экспрессии маркера. Экспрессия клаудина-3 может потенциально рассматриваться в качестве прогностического фактора при раке желудка.

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Nuclear expression of claudin‑3 in human colorectal adenocarcinoma cell lines and tissues

Cited by 19 publications

References 63 publications

Claudins: New Players in Human Fertility and Reproductive System Cancers

Claudins: New Players in Human Fertility and Reproductive System Cancers

FoPA: identifying perturbed signaling pathways in clinical conditions using formal methods

Claudin-3 expression in gastric cancer

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