Nuclear Factor Y Controls the Basal Transcription Activity of the Mouse Platelet‐Derived‐Growth‐Factor β‐Receptor Gene

Abstract

To determine the regulatory mechanism of the expression of the mouse platelet-derived growth factor (PDGF) p-receptor gene, a 1.9-kb 5' flanking genomic fragment was cloned and analyzed. Site

p73 Independent of c-Myc Represses Transcription of Platelet-derived Growth Factor β-Receptor through Interaction with NF-Y

“…In fact, the overexpression of p73␣ yielded a comparable repression as was seen by DNNF-YA alone on the promoter. These results indicate that p73␣ represses the promoter activity by its effect on NF-Y, attenuating the stimulatory effect of NF-Y on the promoter (14).…”

“…Complex to the PDGF ␤-Receptor Promoter-To examine whether NF-Y complex binding to DNA changes during cell cycle, electrophoresis mobility shift assay was performed with a 32 P-labeled double-stranded oligonucleotide probe for PDGF ␤-receptor promoter containing the CCAAT motif (14). Nuclear protein was taken at various time points after serum stimulation from NIH3T3 cells that had been kept in a serum-free medium for 48 h. As shown in Fig.…”

“…The mouse PDGF ␤-receptor promoter contains a CCAAT box, and the NF-Y transcription factor binds to the motif and activates the transcription (13,14). NF-Y consists of NF-YA, NF-YB, and NF-YC subunits that are all necessary for DNA binding (15).…”

Regulation of platelet-derived growth factor signaling by activated p21Ras

“…Sequence analysis of the minimal promoter region revealed a TATA-less promoter with consensus binding sites for known transcription factors including Sp1, GATA, CREB and NF-1. Funa and colleagues, who cloned the PDGF-betaR gene independently, have suggested that a single NF-Y site 60 bp upstream of the transcriptional start site is critical for basal promoter activity (80). Studies are currently underway in our laboratory to identify the putative cisacting elements and trans-acting factors that mediate regulated changes in PDGF-R expression.…”

pRb, Myc and p53 are critically involved in SV40 large T antigen repression of PDGF β-receptor transcription

“…The expression levels of all the constructs were examined and the expression of LT in two different vectors was judged to be similar by immunoblotting 3T3 cell lysates after transfection. For functional promoter assays, the promoter region of mouse PDGF β-receptor was inserted in a luciferase expression vector, pGL3 (Promega) (Ishisaki et al, 1997). The dominant negative mouse NF-YA (DNNFYA) was provided by Dr Mantovani (Mantovani et al, 1994), and Sp1 cDNA was provided by Dr Tjian (Howard Hugh Medical Institute, University of California, CA).…”