NUCLEAR FACTOR-Y: still complex after all these years?

Myers, Zachary A.; Holt, Ben F.

doi:10.1016/j.pbi.2018.05.015

Cited by 93 publications

(93 citation statements)

References 57 publications

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“…The ability of NF‐Y complexes containing non‐LEC1‐type NF‐YB subunits to bind the CCAAT motif and to regulate gene transcription has been extensively studied in yeast, mammals and plants (Dolfini et al ; Zhao et al ; Myers and Holt ). The initial step in NF‐Y complex formation involves dimerization between NF‐YB and NF‐YC through their histone‐fold domains.…”

Section: Lec1 Function Is Modulated By Interactions With Other Transcmentioning

confidence: 99%

“…Because NF‐Y complexes bind CCAAT DNA motifs, it is hypothesized that LEC1 can interact with several other transcription factors and that these interactions specify which set of genes are regulated by LEC1 (Pelletier et al ). Interactions between NF‐Y subunits and other transcription factors have been reported extensively for plants and animals, and these interactions are important to specify the activity of these other transcription factors (Dolfini et al ; Zhao et al ; Myers and Holt ). Here, we discuss interactions between LEC1 and other transcription factors.…”

Section: Lec1 Function Is Modulated By Interactions With Other Transcmentioning

confidence: 99%

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Central role of the LEAFY COTYLEDON1 transcription factor in seed development

Pelletier

Harada

2019

JIPB

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Seed development is a complex period of the flowering plant life cycle. After fertilization, the three main regions of the seed, embryo, endosperm and seed coat, undergo a series of developmental processes that result in the production of a mature seed that is developmentally arrested, desiccated, and metabolically quiescent. These processes are highly coordinated, both temporally and spatially, to ensure the proper growth and development of the seed. The transcription factor, LEAFY COTYLEDON1 (LEC1), is a central regulator that controls several aspects of embryo and endosperm development, including embryo morphogenesis, photosynthesis, and storage reserve accumulation. Thus, LEC1 regulates distinct sets of genes at different stages of seed development. Despite its critical importance for seed development, an understanding of the mechanisms underlying LEC1's multifunctionality is only beginning to be obtained. Recent studies describe the roles of specific transcription factors and the hormones, gibberellic acid and abscisic acid, in controlling the activity and transcriptional specificity of LEC1 across seed development. Moreover, studies indicate that LEC1 acts as a pioneer transcription factor to promote epigenetic reprogramming during embryogenesis. In this review, we discuss the mechanisms that enable LEC1 to serve as a central regulator of seed development.

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Section: Lec1 Function Is Modulated By Interactions With Other Transcmentioning

confidence: 99%

Section: Lec1 Function Is Modulated By Interactions With Other Transcmentioning

confidence: 99%

Central role of the LEAFY COTYLEDON1 transcription factor in seed development

Pelletier

Harada

2019

JIPB

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“…NUCLEAR FACTOR Y complexes are heterotrimeric transcription factors composed of the NF-YC, NF-YB and NF-YA subunits, of which the latter determines the DNA-binding specificity (Baudin et al, 2015;Myers & Holt, 2018). In plants, each of these subunits is encoded by a small family and in legumes several NF-Y-encoding genes display a nodule-enhanced expression profile (Laloum et al, 2013;Baudin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Mutant analysis in the nonlegume Parasponia andersonii identifies NIN and NF‐YA1 transcription factors as a core genetic network in nitrogen‐fixing nodule symbioses

Bu¹,

Rutten²,

Roswanjaya

et al. 2020

New Phytologist

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Summary ●Nitrogen‐fixing nodulation occurs in 10 taxonomic lineages, with either rhizobia or Frankia bacteria. To establish such an endosymbiosis, two processes are essential: nodule organogenesis and intracellular bacterial infection. In the legume–rhizobium endosymbiosis, both processes are guarded by the transcription factor NODULE INCEPTION (NIN) and its downstream target genes of the NUCLEAR FACTOR Y (NF‐Y) complex. ●It is hypothesized that nodulation has a single evolutionary origin c. 110 Ma, followed by many independent losses. Despite a significant body of knowledge of the legume–rhizobium symbiosis, it remains elusive which signalling modules are shared between nodulating species in different taxonomic clades. We used Parasponia andersonii to investigate the role of NIN and NF‐YA genes in rhizobium nodulation in a nonlegume system. ●Consistent with legumes, P. andersonii PanNIN and PanNF‐YA1 are coexpressed in nodules. By analyzing single, double and higher‐order CRISPR‐Cas9 knockout mutants, we show that nodule organogenesis and early symbiotic expression of PanNF‐YA1 are PanNIN‐dependent and that PanNF‐YA1 is specifically required for intracellular rhizobium infection. ●This demonstrates that NIN and NF‐YA1 have conserved symbiotic functions. As Parasponia and legumes diverged soon after the birth of the nodulation trait, we argue that NIN and NF‐YA1 represent core transcriptional regulators in this symbiosis.

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“…These genes are candidates for regulation by DREB2A together with NF-YB2 and NF-YB3 under dehydration and heat stress conditions, respectively ( Supplemental Tables S2 and S3). Among them, several genes have DRE and CCAAT elements on their 1 kb-promoters, and expression of these genes was analyzed by qPCR because eukaryotic NF-Y trimers have been suggested to bind to CCAAT elements (Dolfini et al, 2012;Myers and Holt, 2018). The LEA76 and CYS-RICH TRANS-MEMBRANE MODULE 1 (CYSTM1) genes were candidates regulated by NF-YB2 and DREB2A in the transcriptomic analysis ( Supplemental Table S2) and were significantly up-regulated in NF-YB2-overexpressing plants during dehydration stress.…”

Section: a And B)mentioning

confidence: 99%

“…Nuclear factor Y (NF-Y) is a transcription factor that is widely conserved among eukaryotes (Kumar et al, 2012;Li et al, 2016). A NF-Y trimer is composed of the NF-YA, NF-YB, and NF-YC subunits, and the trimer is known to bind to a CCAAT element on its target gene promoters to regulate their transcription (Myers and Holt, 2018). Humans (Homo sapiens) only have one family protein for each NF-Y subunit.…”

mentioning

confidence: 99%

NF-YB2 and NF-YB3 Have Functionally Diverged and Differentially Induce Drought and Heat Stress-Specific Genes

et al. 2019

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Functional diversification of transcription factors allows the precise regulation of transcriptomic changes under different environmental conditions. The NUCLEAR FACTOR Y (NF-Y) transcription factor comprises three subunits, NF-YA, NF-YB, and NF-YC, and is broadly diversified in plant species, whereas Humans (Homo sapiens) have one protein for each subunit. However, there remains much to be learned about the diversified functions of each subunit in plants. Here, we found that NF-YB2 and NF-YB3, which have the greatest sequence similarity to each other among NF-YB family proteins in Arabidopsis (Arabidopsis thaliana), are functionally diversified and specifically activate dehydration-inducible and heat-inducible genes, according to environmental conditions. Overexpression of NF-YB2 and NF-YB3 specifically enhanced drought and heat stress tolerance, respectively, and each single knockout mutant showed adverse stress-sensitive phenotypes. Transcriptomic analyses confirmed that overexpression of NF-YB2 and NF-YB3 largely affected the transcriptomic changes under dehydration and heat stress conditions, respectively. The DNA-binding profiles of each protein in planta also suggested that dehydration and heat stress increased the DNA-binding activity of NF-YB2 and NF-YB3 to dehydration-inducible and heat stress-inducible target genes, respectively. Moreover, phylogenetic analysis suggested that the NF-YB proteins of angiosperm plants belong to divergent NF-YB2 and NF-YB3 subgroups. These results demonstrate the functional diversification of NF-Y through evolutionary processes and how plants adapt to various abiotic stresses under fluctuating environments.

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NUCLEAR FACTOR-Y: still complex after all these years?

Cited by 93 publications

References 57 publications

Central role of the LEAFY COTYLEDON1 transcription factor in seed development

Central role of the LEAFY COTYLEDON1 transcription factor in seed development

Mutant analysis in the nonlegume Parasponia andersonii identifies NIN and NF‐YA1 transcription factors as a core genetic network in nitrogen‐fixing nodule symbioses

NF-YB2 and NF-YB3 Have Functionally Diverged and Differentially Induce Drought and Heat Stress-Specific Genes

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