Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p

Pries, Ralph; Bömeke, Katrin; Draht, Oliver; Künzler, Markus; Braus, Gerhard H.

doi:10.1007/s00438-003-0955-7

Cited by 12 publications

(12 citation statements)

References 47 publications

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“…The GSP1/gsp1 Δ strain may have a defect in any one or more of several functions that would render the cells sensitive to chemically induced starvation. The most likely effect is on the nuclear localization of Gcn4, which requires Yrb1 (Pries et al 2002), a Ran GTPase–binding protein involved in nuclear import and export (Schlenstedt et al 1995), and the karyopherins Srp1 and Kap95 (Pries et al 2004). …”

Section: Resultsmentioning

confidence: 99%

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

Bae

Seberg

Carroll

et al. 2017

G3 Genes|Genomes|Genetics

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The yeast Saccharomyces cerevisiae responds to amino acid deprivation by activating a pathway conserved in eukaryotes to overcome the starvation stress. We have screened the entire yeast heterozygous deletion collection to identify strains haploinsufficient for growth in the presence of sulfometuron methyl, which causes starvation for isoleucine and valine. We have discovered that cells devoid of MET15 are sensitive to sulfometuron methyl, and loss of heterozygosity at the MET15 locus can complicate screening the heterozygous deletion collection. We identified 138 cases of loss of heterozygosity in this screen. After eliminating the issues of the MET15 loss of heterozygosity, strains isolated from the collection were retested on sulfometuron methyl. To determine the general effect of the mutations for a starvation response, SMM-sensitive strains were tested for the ability to grow in the presence of canavanine, which induces arginine starvation, and strains that were MET15 were also tested for growth in the presence of ethionine, which causes methionine starvation. Many of the genes identified in our study were not previously identified as starvation-responsive genes, including a number of essential genes that are not easily screened in a systematic way. The genes identified span a broad range of biological functions, including many involved in some level of gene expression. Several unnamed proteins have also been identified, giving a clue as to possible functions of the encoded proteins.

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Section: Resultsmentioning

confidence: 99%

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

Bae

Seberg

Carroll

et al. 2017

G3 Genes|Genomes|Genetics

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“…This prediction is consistent with our observation that only the full-length AcVEA polypeptide, and not the truncated version, shows nuclear localization by fluorescent microscopy. Two nuclear localization signals have frequently been reported for proteins from higher as well as lower eukaryotes (2,34). The transcriptional activator Gcn4p from yeast, for example, has been shown to carry two nuclear localization signals, NLS1 and NLS2.…”

Section: Discussionmentioning

confidence: 99%

A Homologue of the Aspergillus velvet Gene Regulates both Cephalosporin C Biosynthesis and Hyphal Fragmentation in Acremonium chrysogenum

Dreyer

Eichhorn²,

Friedlin³

et al. 2007

Appl Environ Microbiol

127

102

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The Aspergillus nidulans velvet (veA) gene encodes a global regulator of gene expression controlling sexual development as well as secondary metabolism. We have identified the veA homologue AcveA from Acremonium chrysogenum, the major producer of the ␤-lactam antibiotic cephalosporin C. Two different disruption strains as well as the corresponding complements were generated as a prelude to detailed functional analysis. Northern hybridization and quantitative real-time PCR clearly indicate that the nucleus-localized AcVEA polypeptide controls the transcriptional expression of six cephalosporin C biosynthesis genes. The most drastic reduction in expression is seen for cefEF, encoding the deacetoxycephalosporine/deacetylcephalosporine synthetase. After 120 h of growth, the cefEF transcript level is below 15% in both disruption strains compared to the wild type. These transcriptional expression data are consistent with results from a comparative and time-dependent high-performance liquid chromatography analysis of cephalosporin C production. Compared to the recipient, both disruption strains have a cephalosporin C titer that is reduced by 80%. In addition to its role in cephalosporin C biosynthesis, AcveA is involved in the developmentally dependent hyphal fragmentation. In both disruption strains, hyphal fragmentation is already observed after 48 h of growth, whereas in the recipient strain, arthrospores are not even detected before 96 h of growth. Finally, the two mutant strains show hyperbranching of hyphal tips on osmotically nonstabilized media. Our findings will be significant for biotechnical processes that require a defined stage of cellular differentiation for optimal production of secondary metabolites.

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“…Gcn4p stability regulation depends on its phosphorylation and occurs exclusively in the yeast nucleus (Pries et al, 2002). Nuclear import of Gcn4p is triggered by the ␣-importin Srp1p and the ␤-importin Kap95p (Pries et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Yeast Gcn4p Stabilization Is Initiated by the Dissociation of the Nuclear Pho85p/Pcl5p Complex

Bömeke

Pries

Korte

et al. 2006

MBoC

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Protein stability of the c-jun-like yeast bZIP transcriptional activator Gcn4p is exclusively controlled in the yeast nucleus. Phosphorylation by the nuclear Pho85p cyclin-dependent protein kinase, a functional homolog of mammalian Cdk5, initiates the Gcn4p degradation pathway in complex with the cyclin Pcl5p. We show that the initial step in Gcn4p stabilization is the dissociation of the Pho85p/Pcl5p complex. Pcl7p, another nuclear and constantly present cyclin, is required for Gcn4p stabilization and is able to associate to Pho85p independently of the activity of the Gcn4p degradation pathway. In addition, the nuclear cyclin-dependent Pho85p kinase inhibitor Pho81p is required for Gcn4p stabilization. Pho81p only interacts with Pcl5p when Gcn4p is rapidly degraded but constitutively interacts with Pcl7p. Our data suggest that Pcl7p and Pho81p are antagonists of the Pho85p/Pcl5p complex formation in a yet unknown way, which are specifically required for Gcn4p stabilization. We suggest that dissociation of the Pho85p/Pcl5p complex as initial step in Gcn4p stabilization is a prerequisite for a shift of equilibrium to an increased amount of the Pho85p/Pcl7p complexes and subsequently results in decreased Gcn4p phosphorylation and therefore increased stability of the transcription factor.

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Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p

Cited by 12 publications

References 47 publications

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

A Homologue of the Aspergillus velvet Gene Regulates both Cephalosporin C Biosynthesis and Hyphal Fragmentation in Acremonium chrysogenum

Yeast Gcn4p Stabilization Is Initiated by the Dissociation of the Nuclear Pho85p/Pcl5p Complex

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