Nuclear Inclusions Developing in the Kidneys of Australian Opossums Maintained Under Laboratory Conditions

Hurst, E. Weston; Cooke, Barbara Ternent; Mawson, Jessica; Melvin, Perry D.

doi:10.1038/icb.1943.19

Aust J Exp Biol Med

1943

DOI: 10.1038/icb.1943.19

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Nuclear Inclusions Developing in the Kidneys of Australian Opossums Maintained Under Laboratory Conditions

E. Weston Hurst

Barbara Ternent Cooke

Jessica Mawson

et al.

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Cited by 4 publications

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“…Sections of kidney from diagnostic necropsies on 169 macropods of 16 species from north Queensland and Papua New Guinea were available for examination. Species and number of specimens examined were rufous bettong (Aepyptymnus rujscens) (4), Lumholtz tree kangaroo (Dendrolagus lumholtzi) (2), Dorcopsulus vanheurni (2), musky rat kangaroo (Hypsiprymnodon moschatus) (I), spectacled hare wallaby (Lagorchestes conspicillatus) (6), agile wallaby (Macropus agilis) (37), antelopine kangaroo ( M antilopinus) (I), black stripe wallaby ( M dorsalis) (7), eastern grey kangaroo ( M giganteus) ( (9, and species of macropod not recorded (7). Macropods were both captive and wild, ranging in age from pouch young to mature adults.…”

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confidence: 99%

Idiopathic intranuclear inclusion bodies in the renal epithelium of macropods

Speare

Donovan²,

Skerratt

et al. 1997

Aust Veterinary J

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1 1 ntranuclear inclusion bodies in the renal epithelium of I domestic animals are usually caused by heavy metal toxicosis or viruses.' Intranuclear inclusions of unknown aetiology and apparently of no clinical significance have been reported in the renal epithelium of the common brushtailed possum(Trichosurus vulpecula).? Here we describe the appearance of idiopathic intranuclear inclusions, similar to those in the brushtail possum, in the renal tubular epithelium of several species of macropods and differentiate them from intranuclear inclusion bodies caused by viruses and lead toxicity. Sections of kidney from diagnostic necropsies on 169 macropods of 16 species from north Queensland and Papua New Guinea were available for examination. Species and number of specimens examined were rufous bettong (Aepyptymnus rujscens) (4), Lumholtz tree kangaroo (Dendrolagus lumholtzi) (2), Dorcopsulus vanheurni (2), musky rat kangaroo (Hypsiprymnodon moschatus) (I), spectacled hare wallaby (Lagorchestes conspicillatus) (6), agile wallaby (Macropus agilis) (37), antelopine kangaroo ( M antilopinus) (I), black stripe wallaby ( M dorsalis) (7), eastern grey kangaroo ( M giganteus) (48), pretty face wallaby (Mparryr) (4), eastern wallaroo ( M robustus) (5), red kangaroo ( M rufis) (12), bridled nail-tail wallaby (Onychogalea fiaenatu) (4), northern nail-tail wallaby ( 0 ungu;fera) ( lo), Palm Island rock wallaby (Petrogale assirnilis) (2), unadorned rock wallaby ( P inornata) (8), purple neck rock wallaby ( P purpuriecollis) (3), red-legged pademelon ( Thylogale stigmatila) (I), swamp wallaby (Wallabia bicolor) (9, and species of macropod not recorded (7). Macropods were both captive and wild, ranging in age from pouch young to mature adults. The kidneys were fixed in 10% buffered neutral formalin and embedded in paraffin. Sections were cut at 5 p m and stained with haematoxylin and eosin. Sections from a M dorsalis with typical lesions were also stained using Ziehl-Neelsen, von Kossa technique and periodic acid Schiff (PAS) methods for light microscopy.' Sections of formalin fixed kidney from the same M dorsalis were removed from the paraffin block and prepared for electron microscopy and X-ray dispersion analysis. The tissue was hydrated, given additional fixation in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer for 2 h, then 1% osmium, dehydrated and embedded in Spurr's resin. Sections of 200 nm thickness were stained in methanol uranyl acetate for 10 min. The sections were carbon-coated to 20 nm on copper grids and X-ray dispersion analysis was conducted at 80 kV. Dimensions of nuclei and inclusion bodies in haematoxylin and eosin stained sections from seven macropods were measured aCurrent address: Department of Public Health and Tropical Medicine bPrevious address: Graduate School of Tropical Veterinary Science CDepartment of Biomedical and Tropical Veterinary Science using a calibrated eyepiece graticule. Statistical analyses were performed using Statistix" program ( N H Analytical Software) ...

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mentioning

confidence: 99%

Idiopathic intranuclear inclusion bodies in the renal epithelium of macropods

Speare

Donovan²,

Skerratt

et al. 1997

Aust Veterinary J

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Localized Cytomegalic Inclusion Disease of Lacrimal Glands in the NMRI-D Strain Caries-susceptible Rat

1960

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In a previous report,' the presence of a localized cytomegalic inclusion disease in exorbital and intraorbital lacrimal glands of male laboratory rats was described. This condition was observed in adult males of eight laboratory colonies but was not seen in any wild urban or rural Norway rats. A brief summary of this report is in order.For a recent toxicological study (unpublished), several hunded adult male SpragueDawley rats were purchased from a Midwestern firm. Routine histologic survey of tissues from the head and neck showed invasion of lacrimal glands by a cytomegalic inclusion disease. All other general body-organ systems were free of inclusion involvement. Certain distinctive features described by Wyatt2 as essential to complete morphologic diagnosis were satisfied, namely, eosinophilic inclusion bodies, margination of nuclear chromatin, and specific cellular gigantism. For comparative purposes, attempts were made to procure normal lacrimal tissue. Six adult male and female rats of various ages from each strain of Naval Medical Research Institute stock, i.e., Long-Evans, Sprague-Dawley, and the closed colony NMRI-Dental Caries-Susceptible, were selected at random and prepared for histologic evaluation. All strains showed extensive lacrimal involvement by inclusion disease but only in males. Again, all other organ systems were free of any cytomegalic inclusion disease. Sample male rats, four to each group, were procured from four other laboratories in various geogaphic locations in the United States. Inclusion disease was present in all lacrimal glands, although variations in inclusion morphology and lesion severity were observed.

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Cytomegalic Lesions in Australian Marsupials

Munday

Obendorf

1983

Journal of Wildlife Diseases

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Nuclear Inclusions Developing in the Kidneys of Australian Opossums Maintained Under Laboratory Conditions

Cited by 4 publications

References 3 publications

Idiopathic intranuclear inclusion bodies in the renal epithelium of macropods

Idiopathic intranuclear inclusion bodies in the renal epithelium of macropods

Localized Cytomegalic Inclusion Disease of Lacrimal Glands in the NMRI-D Strain Caries-susceptible Rat

Cytomegalic Lesions in Australian Marsupials

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