1997
DOI: 10.1016/s0014-5793(97)00183-x
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Nuclear localisation of calreticulin in vivo is enhanced by its interaction with glucocorticoid receptors

Abstract: © 1997 Federation of European Biochemical Societies.

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Cited by 95 publications
(67 citation statements)
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“…Alkaline phosphataseconjugated goat anti-rabbit antibody was from Bio-Rad. Recombinant baculoviruses expressing ER p57 , CRT, CNX, PDI, or BiP were prepared as described previously (32)(33)(34)(35)(36). Antibodies against each of the chaperones were from StressGen (Victoria, Canada).…”
Section: Methodsmentioning
confidence: 99%
“…Alkaline phosphataseconjugated goat anti-rabbit antibody was from Bio-Rad. Recombinant baculoviruses expressing ER p57 , CRT, CNX, PDI, or BiP were prepared as described previously (32)(33)(34)(35)(36). Antibodies against each of the chaperones were from StressGen (Victoria, Canada).…”
Section: Methodsmentioning
confidence: 99%
“…Despite this, the protein has been reported at other intracellular locations such as the nucleus [23,24], as well as the cell surface, e.g. after inflammatory stimulation of neutrophils [25], and, as stated earlier, extracellularly, e.g.…”
Section: Mechanisms Of Calreticulin Release From Cells and Extracellumentioning
confidence: 95%
“…Proteins were characterized using a primary rabbit polyclonal anti-GFP antibody, generated against a GFP-calreticulin fusion protein [26] and a secondary goat anti-rabbit antibody conjugated to horseradish peroxidase (Bio-Rad). Blots were developed using the enhanced chemiluminescence (ECL2) system (Pierce).…”
Section: Cos-7 or Hela Cells (European Collection Of Animal Cellmentioning
confidence: 99%
“…Post-transfection (24 h) the cells were split on to four 16-mm# coverslips placed in 12-well dishes followed the next day by fixation in 4 % formaldehyde, and if necessary, processed for immunocytochemical analysis [22]. Colocalization of Cx and GFP was confirmed by staining the cells with the relevant primary anti-Cx antibodies : for Cx26 Gap 28H against the intracellular loop of Cx26 [27], for Cx32 a monoclonal antibody to an intracellular loop sequence was used (Chemicon) and a polyclonal rabbit antibody against calreticulin was used as an ER marker [26]. The secondary antibody was goat anti-rabbit or goat anti-mouse conjugated to Cy3 (Sigma) as required.…”
Section: Cos-7 or Hela Cells (European Collection Of Animal Cellmentioning
confidence: 99%