Nuclear Magnetic Relaxation and the Biological Membrane

Lee, A. G.; Birdsall, N. J. M.; Metcalfe, James C.

doi:10.1007/978-1-4615-8960-0_1

Cited by 44 publications

(49 citation statements)

References 268 publications

(375 reference statements)

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“…Recent efforts have been directed toward developing spectroscopic probes that would circumvent the possible perturbations in the bilayer produced by the bulky nitroxide radical. Nuclear Magnetic Resonance (NMR) spectroscopy is a natural tool for this purpose and there has been considerable effort using IH, 2H, 13C, and 31p probes which are either endogenous or incorporated into the lipid molecule (3)(4)(5)(6)(7)(8)(9)(10)(11). These efforts constitute the subject of the present review.…”

Section: Introductionmentioning

confidence: 99%

NMR Studies of Membrane Structure and Dynamics

Bocian¹,

Chan²

1978

Annu. Rev. Phys. Chem.

113

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Section: Introductionmentioning

confidence: 99%

NMR Studies of Membrane Structure and Dynamics

Bocian¹,

Chan²

1978

Annu. Rev. Phys. Chem.

113

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“…The present paper (i) summarizes the results of 2H T1 studies of multilamellar dispersions of 1,2-dipalmitoylphosphatidylcholine (Pam2-PtdCho; 1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and (ii) describes recent '3C T1 studies of vesicles of Pam2-PtdCho at seven different magnetic field strengths (i.e., resonance frequencies). Previous 13C T1 studies of Pam2-PtdCho vesicles have been limited to one magnetic field strength (8,11,22,23). Both the 2H and the 13C T, data can be explained best in terms of a dominant contribution to the acyl chain relaxation from collective bilaver modes, as described below.…”

mentioning

confidence: 96%

“…Perhaps the foremost progress in recent years has been made in applications of NMR lineshape analysis to studies of the molecular ordering and conformations of membranous lipids (3)(4)(5)(6)(7). Yet, in spite of early promise (8)(9)(10)(11), the interpretation of nuclear spin relaxation experiments has not progressed similarly and, in fact, has remained an outstanding problem in membrane biophysics for more than a decade (cf. ref.…”

mentioning

confidence: 99%

“…The latter observations are consistent with recent natural abundance '3C T-1studies that use cross-polarization/magicangle spinning techniques (31). The and 126 MHz (11.7 tesla), together with resonance assignments (8,11) and plots of (NT,)-1 vs. chain position, where N is the number of directly bonded protons. Note particularly the non-Lorenztian shape of the acyl chain envelope profile from C-4 to C-13, which is resolved into three or more components at the higher magnetic field strengths (7.05-11.7 tesla), and the well-resolved doublets from those carbons near the chain ends, which are due either to chain inequivalence (4,6) or to chemical shift differences between the two monolayers of the unilamellar vesicles.…”

mentioning

confidence: 99%

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New view of lipid bilayer dynamics from 2H and 13C NMR relaxation time measurements.

Brown¹,

Ribeiro²,

Williams³

1983

Proc. Natl. Acad. Sci. U.S.A.

148

118

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Natural abundance 13C spin-lattice (TI) relaxation time measurements are reported for unilamellar vesicles of 1,2-dipalmitoylphosphatidylcholine (1,2-dipalmitoyl-sn-glycero-3-phosphocholine), in the liquid crystalline phase, at magnetic field strengths of 1. 40, 1.87, 2.35, 4.23, 7.05, 8.45, and 11.7 tesla (resonance frequencies of 15.0, 20.0, 25.1, 45.3, 75.5, 90.5, and 126 MHz, respectively), and the results are compared to previous 2H T, studies of multilamellar dispersions. For both the 13C and 2H T, studies, a dramatic frequency dependence of the relaxation was observed. At superconducting magnetic field strengths (4.23-11.7 tesla), plots of the 13C TF1 relaxation rates as a function of acyl chain segment position clearly reveal the characteristic "plateau" signature of the liquid crystalline phase, as found previously from 2H NMR studies. The dependence of Tj ' on ordering, determined previously from 2H NMR, and the Tj' dependence on frequency, determined from both '3C and 2H NMR studies, suggest that a unified picture of the bilayer molecular dynamics can be provided by a simple relaxation law of the form T' l ATf + BS2 H W-'1/2. In the above expression, A and B are constants, SCH (=SC-D) is the bond segmental order parameter, and of is the nuclear Larmor frequency. The first (A) term includes contributions from fast, local segmental motions characterized by the effective correlation time Tf, whereas the second (B) term describes slower, collective fluctuations in the local ordering. The value of Tf 10-11 sec, obtained by extrapolating Tj' to infinite frequency, suggests that the segmental microviscosity of the bilayer hydrocarbon region does not differ appreciably from that of the equivalent n-paraffinic liquids of similar chain length.NMR techniques were among the first biophysical methods to be applied to lipid bilayers and biological membranes (1, 2). Perhaps the foremost progress in recent years has been made in applications of NMR lineshape analysis to studies of the molecular ordering and conformations of membranous lipids (3-7). Yet, in spite of early promise (8-11), the interpretation of nuclear spin relaxation experiments has not progressed similarly and, in fact, has remained an outstanding problem in membrane biophysics for more than a decade (cf. ref. 12). The bulk of previous work has involved spin-lattice (TI) relaxation time measurements, which are sensitive to details of the molecular motions in the MHz region. Perhaps the major justification for T, relaxation studies of membranes is their dual character as both solid-like and liquid-like materials. The analogies to simpler liquid crystals point to the necessity of obtaining both static and dynamic information in defining these systems and of distinguishing membranes from other classes of biological macromolecules, such as the globular and fibrous proteins and the nucleic acids. These latter biopolymers, while also rich in dvnamic behavior (13,14), appear to have fairly well-defined average structures that can be directly re...

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“…For dipalmitoyl lecithin, T1 values have been reported for 6 of the 16 13C-resonances of the fatty acid chain. The T1 values of the carbon nuclei increase from the carboxyl end towards the terminal methyl of the fatty acid chains [3,4] and these values can be explained simply in terms of increasing motion about C-C bonds in the alkyl chains [5,6]. In other cases where the rate of overall rotation is comparable to internal motion, nearly equivalent T1 values are obtained for each carbon atom in the molecule [7].…”

Section: Introductionmentioning

confidence: 99%

¹³C NMR relaxation of trilauroin and its cyclopentanoid analogs

Gallo

1977

FEBS Letters

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Nuclear Magnetic Relaxation and the Biological Membrane

Cited by 44 publications

References 268 publications

NMR Studies of Membrane Structure and Dynamics

NMR Studies of Membrane Structure and Dynamics

New view of lipid bilayer dynamics from 2H and 13C NMR relaxation time measurements.

¹³C NMR relaxation of trilauroin and its cyclopentanoid analogs

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Nuclear Magnetic Relaxation and the Biological Membrane

Cited by 44 publications

References 268 publications

NMR Studies of Membrane Structure and Dynamics

NMR Studies of Membrane Structure and Dynamics

New view of lipid bilayer dynamics from 2H and 13C NMR relaxation time measurements.

13C NMR relaxation of trilauroin and its cyclopentanoid analogs

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¹³C NMR relaxation of trilauroin and its cyclopentanoid analogs