Nuclear Magnetic Resonance: A Tool for Malaria Diagnosis?

Karl, Stephan; Gutiérrez, Lucía; House, Michael J.; Davis, Timothy M. E.; Pierre, Timothy G. St.

doi:10.4269/ajtmh.2011.11-0299

Cited by 16 publications

(16 citation statements)

References 17 publications

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“…We have shown that concentrating the RBCs via standard centrifugation and exploiting UET technique has given a signal sensitivity enhancement, compared with work reported by Karl et al ( Supplementary Fig. 10) 25 .…”

Section: Discussionmentioning

confidence: 45%

“…MRR detection was carried out on the concentrated (near 100% hematocrit) band of RBCs, which allows us to remove the possible confounding effect of variations in blood hematocrit number among individuals. This important strategy enhanced the MRR sensitivity drastically, in contrast to the work by Karl and coworkers 25 , where centrifugation is not used. We discuss the effect of centrifugation on the of sensitivity detection ( Supplementary Fig.…”

Section: Methodsmentioning

confidence: 81%

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Micromagnetic resonance relaxometry for rapid label-free malaria diagnosis

Peng

Kong

et al. 2014

Nat Med

122

144

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We report a new technique for sensitive, quantitative and rapid detection of Plasmodium spp.-infected red blood cells (RBCs) by means of magnetic resonance relaxometry (MRR). During the intraerythrocytic cycle, malaria parasites metabolize large amounts of cellular hemoglobin and convert it into hemozoin crystallites. We exploit the relatively large paramagnetic susceptibility of these hemozoin particles, which induce substantial changes in the transverse relaxation rate of proton nuclear magnetic resonance of RBCs, to infer the 'parasite load' in blood. Using an inexpensive benchtop 0.5-Tesla MRR system, we show that with minimal sample preparatory steps and without any chemical or immunolabeling, a parasitemia level of fewer than ten parasites per microliter in a volume below 10 μl of whole blood is detected in a few minutes. We demonstrate this method both for cultured Plasmodium falciparum parasites and in vivo with Plasmodium berghei-infected mice.

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Section: Discussionmentioning

confidence: 45%

Section: Methodsmentioning

confidence: 81%

Micromagnetic resonance relaxometry for rapid label-free malaria diagnosis

Peng

Kong

et al. 2014

Nat Med

122

144

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“…The first method 15 , 17 uses the magnetic moment of the pigment crystals to align them in a specific direction which makes them magnetically driven optical polarizers. The second method, evaluated by two different groups 18 , 19 , tries to take advantage of the paramagnetism of hemozoin through the effect that such magnetic crystals can have on Nuclear Magnetic Resonance (NMR) properties of neighbouring water protons in whole blood. Indeed, magnetic nano- and micro-particles cause a shortening of the relaxation times T 1 and T 2 of water protons, a phenomenon which is at the origin of the use of iron oxide particles as Magnetic Resonance Imaging (MRI) contrast agents.…”

Section: Introductionmentioning

confidence: 99%

NMR relaxation properties of the synthetic malaria pigment β-hematin

Gossuin

Ndjolo

Vuong

et al. 2017

Sci Rep

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200 million patients suffer from malaria, a parasitic disease caused by protozoans of the genus Plasmodium. Reliable diagnosis is crucial since it allows the early detection of the disease. The development of rapid, sensitive and low-cost diagnosis tools is an important research area. Different studies focused on the detection of hemozoin, a major by-product of hemoglobin detoxification by the parasite. Hemozoin and its synthetic analog, β-hematin, form paramagnetic crystals. A new detection method of malaria takes advantage of the paramagnetism of hemozoin through the effect that such magnetic crystals have on Nuclear Magnetic Resonance (NMR) relaxation of water protons. Indeed, magnetic microparticles cause a shortening of the relaxation times. In this work, the magnetic properties of two types of β-hematin are assessed at different temperatures and magnetic fields. The pure paramagnetism of β-hematin is confirmed. The NMR relaxation of β–hematin suspensions is also studied at different magnetic fields and for different echo-times. Our results help to identify the best conditions for β–hematin detection by NMR: T 2 must be selected, at large magnetic fields and for long echo-times. However, the effect of β-hematin on relaxation does not seem large enough to achieve accurate detection of malaria without any preliminary sample preparation, as microcentrifugation.

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“…combined both the magnetic properties and anisotropic light scattering into am agnetoopticald etection technique. [29] Karl et al suggested that nuclear magnetic resonance spectroscopy [30] could be used to detect the ferric Fe III magnetics pin in hemozoin, and recently Peng et al proposed micromagnetic resonance relaxometry [31] to detect the magnetic susceptibility of ferric Fe III in hemozoin. Additionally,t he fundamental atomistic vibrational properties of hemozoin have resulted in various hemozoin identification techniques, such as FT-IR spectroscopy,resonance Raman spectroscopy( RRS), and surface-enhanced resonance Raman scattering (SERRS).…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Electronic Structure, Spin States, Optical and Vibrational Spectra of Malaria Pigment

Ali

Oppeneer

2015

Chemistry A European J

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A detailed knowledge of the electronic structure and magnetic and optical properties of hemozoin, the malaria pigment, is essential for the design of effective antimalarial drugs and malarial diagnosis. By employing state-of-the-art electronic structure calculations, we have performed an in-depth investigation of the malaria pigment. Specifically, molecular bond lengths and spin states of the two Fe(III) heme centers and their exchange interaction, the UV/Vis absorption spectrum, and the IR vibrational spectra were calculated and compared with available experimental data. Our density functional theory (DFT)-based calculations predict a singlet ground spin state that stems from an S=5/2 spin state on each of the Fe heme centers with a very weak antiferromagnetic exchange interaction between them. Our theoretical UV/Vis and IR spectra provide explanations for various spectroscopic studies of hemozoin and β-hematin (a synthetic analogue of hemozoin). A good comparison of calculated and measured properties demonstrates the convincing unveiling of the electronic structure of the malaria pigment. Based on the predicted vibrational spectra, we propose a unique spectral band from the nuclear resonance vibrational spectroscopy (NRVS) results that could be used as a key fingerprint for malarial detection.

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Nuclear Magnetic Resonance: A Tool for Malaria Diagnosis?

Cited by 16 publications

References 17 publications

Micromagnetic resonance relaxometry for rapid label-free malaria diagnosis

Micromagnetic resonance relaxometry for rapid label-free malaria diagnosis

NMR relaxation properties of the synthetic malaria pigment β-hematin

Unraveling the Electronic Structure, Spin States, Optical and Vibrational Spectra of Malaria Pigment

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