Nuclear Magnetic Resonance Metabolomics of Symbioses between Bacterial Vaginosis-Associated Bacteria

Horrocks, Victoria; Hind, Charlotte K.; Wand, Matthew E.; Fady, Paul-Enguerrand; Chan, Joseph L.-K.; Hopkins, Jade C; Houston, Georgina L; Tribe, Rachel; Sutton, J. Mark; Mason, A. James

doi:10.1128/msphere.00166-22

Cited by 8 publications

(8 citation statements)

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“…revealed a symbiotic and commensal relationship among bacteria commonly associated with BV infection and sPTB ( 102 ). Notably, Prevotella spp., such as P. bivia , a group of bacteria frequently linked to BV infections and sPTB, exhibited distinctive symbiotic connections with Peptostreptococcus anaerobius and G. vaginalis ( 102 ). P. bivia plays a pivotal role in this relationship by significantly releasing metabolites actively utilized by other BV-associated bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…These metabolites include succinate, fumarate, alanine, glutamate, glycine, methionine, phenylalanine, proline, valine, and uracil, highlighting the crucial role of P. bivia in cross-feeding within the cervicovaginal environment. Furthermore, P. bivia and P. anaerobius maintain a commensal relationship within cervicovaginal environment where P. bivia provides amino acids (threonine, tyrosine, phenylalanine, and proline), especially proline, to P. anaerobius , promoting increased glucose uptake and acetate production from glucose ( 102 ). It is thought that G. vaginalis , by aiding the ascension of P. bivia , enhances its invasive potential in the uterus ( 103 ).…”

Section: Introductionmentioning

confidence: 99%

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Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Onyango,

Mi,

Koech

et al. 2023

Front. Immunol.

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Differences in the cervicovaginal microbiota are associated with spontaneous preterm birth (sPTB), a significant cause of infant morbidity and mortality. Although establishing a direct causal link between cervicovaginal microbiota and sPTB remains challenging, recent advancements in sequencing technologies have facilitated the identification of microbial markers potentially linked to sPTB. Despite variations in findings, a recurring observation suggests that sPTB is associated with a more diverse and less stable vaginal microbiota across pregnancy trimesters. It is hypothesized that sPTB risk is likely to be modified via an intricate host-microbe interactions rather than due to the presence of a single microbial taxon or broad community state. Nonetheless, lactobacilli dominance is generally associated with term outcomes and contributes to a healthy vaginal environment through the production of lactic acid/maintenance of a low pH that excludes other pathogenic microorganisms. Additionally, the innate immunity of the host and metabolic interactions between cervicovaginal microbiota, such as the production of bacteriocins and the use of proteolytic enzymes, exerts a profound influence on microbial populations, activities, and host immune responses. These interplays collectively impact pregnancy outcomes. This review aims to summarize the complexity of cervicovaginal environment and microbiota dynamics, and associations with bacterial vaginosis and sPTB. There is also consideration on how probiotics may mitigate the risk of sPTB and bacterial vaginosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Onyango,

Mi,

Koech

et al. 2023

Front. Immunol.

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“…Analytical methods used for metabolomics analysis included electrophoresis and thin layer chromatography (TLC) prior to 2000, as well as nuclear magnetic resonance spectroscopy (NMR), enzyme‐linked immunosorbent assay (ELISA) and mass spectrometry (MS) methods conducted after 2013. Overall, MS‐based techniques were more common for untargeted metabolomic profiling 30,48,49 …”

Section: Resultsmentioning

confidence: 99%

“…Biological samples were predominantly were more common for untargeted metabolomic profiling. 30,48,49…”

Section: Study Inclusion and Characteristicsmentioning

confidence: 99%

Metabolic signature for a dysbiotic microbiome in the female genital tract: A systematic review and meta‐analysis

Gill,

Schwecht,

Rahman

et al. 2023

American J Rep Immunol

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BackgroundThe vaginal microbiome (VMB) is a critical determinant of reproductive health, where a microbial shift towards a dysbiotic environment has implications for susceptibility to, and clinical presentation of sexually transmitted infections (STIs). Metabolomic profiling of the vaginal microenvironment has led to the identification of metabolic responses to clinical conditions of dysbiosis. However, no studies have examined metabolic markers that are common across conditions and can serve as a signature for vaginal dysbiosis.Method of StudyWe have conducted a comprehensive systematic review and meta‐analysis to identify consistently deregulated metabolites along with their impact on host and microbial metabolism during dysbiosis. We employed two complementary approaches including a vote counting analysis for all eligible studies identified in the systematic review, in addition to a meta‐analysis for a subset of studies with sufficient available data. Significantly deregulated metabolites were then selected for pathway enrichment analysis.ResultsOur results revealed a total of 502 altered metabolites reported across 10 dysbiotic conditions from 16 studies. Following a rigorous, collective analysis, six metabolites which were consistently downregulated and could be generalized to all dysbiotic conditions were identified. In addition, five downregulated and one upregulated metabolite was identified from a bacterial vaginosis (BV) focused sub‐analysis. These metabolites have the potential to serve as a metabolic signature for vaginal dysbiosis. Their role in eight altered metabolic pathways indicates a disruption of amino acid, carbohydrate, and energy metabolism during dysbiosis.ConclusionBased on this analysis, we propose a schematic model outlining the common metabolic perturbations associated with vaginal dysbiosis, which can be potential targets for therapeutics and prophylaxis.

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“…• Slow technique • High susceptibility to impurities within the samples • Low coverage in protein quantification • A highly specific protein database requirement • High quantity of protein content required and the endometrial microbiome. 30,138 Examples of these studies approach physiological and pathological states, such as pregnancy and puerperium, [139][140][141] preterm birth, 142 bacterial vaginosis, 143 endometriosis, 144 recurrent implantation failure. 145,146 While scarce, there are also some studies on male fluids and tissues (i.e., semen).…”

Section: Advantagesmentioning

confidence: 99%

Exploring the Microbiome in Human Reproductive Tract: High-Throughput Methods for the Taxonomic Characterization of Microorganisms

Salas-Espejo,

Terrón-Camero,

Ruiz

et al. 2023

Semin Reprod Med

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Microorganisms are important due to their widespread presence and multifaceted roles across various domains of life, ecology, and industries. In humans, they underlie the proper functioning of multiple systems crucial to well-being, including immunological and metabolic functions. Emerging research addressing the presence and roles of microorganisms within human reproduction is increasingly relevant. Studies implementing new methodologies (e.g., to investigate vaginal, uterine, and semen microenvironments) can now provide relevant insights into fertility, reproductive health, or pregnancy outcomes. In that sense, cutting-edge sequencing techniques, as well as others such as meta-metabolomics, culturomics, and meta-proteomics, are becoming more popular and accessible worldwide, allowing the characterization of microbiomes at unprecedented resolution. However, they frequently involve rather complex laboratory protocols and bioinformatics analyses, for which researchers may lack the required expertise. A suitable pipeline would successfully enable both taxonomic classification and functional profiling of the microbiome, providing easy-to-understand biological interpretations. However, the selection of an appropriate methodology would be crucial, as it directly impacts the reproducibility, accuracy, and quality of the results and observations. This review focuses on the different current microbiome-related techniques in the context of human reproduction, encompassing niches like vagina, endometrium, and seminal fluid. The most standard and reliable methods are 16S rRNA gene sequencing, metagenomics, and meta-transcriptomics, together with complementary approaches including meta-proteomics, meta-metabolomics, and culturomics. Finally, we also offer case examples and general recommendations about the most appropriate methods and workflows and discuss strengths and shortcomings for each technique.

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Nuclear Magnetic Resonance Metabolomics of Symbioses between Bacterial Vaginosis-Associated Bacteria

Cited by 8 publications

References 51 publications

Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Metabolic signature for a dysbiotic microbiome in the female genital tract: A systematic review and meta‐analysis

Exploring the Microbiome in Human Reproductive Tract: High-Throughput Methods for the Taxonomic Characterization of Microorganisms

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