Nuclear Magnetic Resonance Spectroscopic Based Studies of the Metabolism of Black Tea Polyphenols in Humans

Daykin, Clare A.; Duynhoven, John van; Groenewegen, A.; Dachtler, Markus; Amelsvoort, J.M.M. van; Mulder, T.P.J.

doi:10.1021/jf048439o

Cited by 103 publications

(104 citation statements)

References 34 publications

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“…Non-transformed metabolites are excreted in urine and feces. Hippurate is a known urinary excretion product associated with consumption of black tea (54,55). Gavaghan et al (56) have reported dietary effects on the urinary excretion of hippuric acid and chlorogenic acid metabolites in a genetically homogeneous population of rats maintained under identical conditions and have pointed out the possible influence of gut microflora on the metabolism.…”

Section: Discussionmentioning

confidence: 99%

¹H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome

et al. 2007

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Current research increasingly recognizes the human gut microbiome as a metabolically versatile biological 'digester' that plays an essential role in regulating the host metabolome. Gut microbiota recover energy and biologically active molecules from food that would otherwise be washed out of the intestinal tract without benefit. In this study, a protocol for NMR-based metabolite profiling has been developed to access the activity of the microbiome. The physicochemical properties of fecal metabolites have been found to strongly affect the reproducibility and coverage of the profiles obtained. Metabolite profiles generated by water and methanol extraction of lyophilized feces are reproducible and comprise a variety of different compounds including, among others, short-chain fatty acids (e.g. acetate, propionate, butyrate, isobutyrate, isovalerate, malate), organic acids (e.g. succinate, pyruvate, fumarate, lactate), amino acids, uracil, trimethylamine, ethanol, glycerol, glucose, phenolic acids, cholate, and lipid components. The NMR profiling approach was validated on fecal samples from a double-blinded, placebo-controlled, randomized cross-over study, in which healthy human subjects consumed a placebo and either a grape juice extract or a mix of grape juice and wine extract over a period of 4 weeks, each. The considerable inter- and intra-individual variability observed originates in the first instance from variable metabolite concentrations rather than from variable metabolite compositions, suggesting that different colonic flora share general biochemical characteristics metabolizing different substrates to specific metabolic patterns. Whereas the grape juice extract did not induce changes in the metabolite profiles as compared with the placebo, the mixture of grape juice and wine extract induced a reduction in isobutyrate, which may indicate that polyphenols are able to modulate the microbial ecology of the gut.

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Section: Discussionmentioning

confidence: 99%

¹H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome

et al. 2007

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“…Within the field of nutrition, NMR-based metabonomics is slowly emerging as a tool for elucidating how diet elicits changes in the metabolite profile. Applications of NMRbased metabonomics on blood plasma and urine samples for elucidating the biochemical effects of dietary bioactive compounds such as epicatechin [14], isoflavones [15], chamomile [16] and tea phenols [17] have been reported. In addition, it has also been demonstrated that NMR-based metabonomics is valuable for elucidating how wholegrain and cereal fractions alter the urine and plasma metabolite profile of pigs [18] and rats [19].…”

Section: Introductionmentioning

confidence: 99%

NMR‐based metabonomics reveals that plasma betaine increases upon intake of high‐fiber rye buns in hypercholesterolemic pigs

Bertram¹,

Malmendal

Nielsen

et al. 2009

Molecular Nutrition Food Res

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This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.

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“…Hippurate has also been reported as a metabolic end product of plant phenols and flavonoids by the gut microflora (Daykin et al 2005;Mulder et al 2005;Nicholson et al 2005;Van Dorsten et al 2006;Wang et al 2005) and hence, it can also be speculated that hippurate is partially a metabolic signature by the gut microflora of the subjects participating in the present study.…”

Section: Associations Between Dietary Fibre and Human Urinary Metabolmentioning

confidence: 67%

Assessment of dietary exposure related to dietary GI and fibre intake in a nutritional metabolomic study of human urine

Rasmussen

Winning²,

Savorani

et al. 2011

Genes Nutr

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There is a need for a tool to assess dietary intake related to the habitual dietary glycaemic index (GI) and fibre in groups with large numbers of individuals. Novel metabolite-profiling techniques may be a useful approach when applied to human urine. In a long-term, controlled dietary intervention study, metabolomics were applied to assess dietary patterns. A targeted approach was used to evaluate the effects on urinary C-peptide excretion caused by the dietary treatments. Seventy-seven overweight subjects followed an 8-week low-calorie diet (LCD) and were then randomly assigned to a high-GI or low-GI diet for 6 month during which they completed 24-h urine collections at baseline (prior to the 8-week LCD) and after randomisation to the dietary intervention, at month 1, 3 and 6, respectively. Metabolite profiling in 24-h urine was performed by 1 H NMR and chemometrics. Partial least squares (PLS) analysis indicated that urinary formate could discriminate between high-GI and low-GI diets (correlation coefficient r = 0.82), and this finding was confirmed statistically (P = 0.01). PLS analysis also indicated that urinary hippurate could be associated with fibre intake, but this finding was not confirmed statistically. No associations between GI and urinary C-peptide were found. Our results emphasise that application of metabolomics is useful in the assessment of dietary exposure related to dietary GI and fibre seen at group level in a nutritional metabolomic study of human urine. As our design allowed for large variations in individually selected food items, biomarkers identified at group level may be interpreted as more general and robust markers, largely not confounded with markers from single dietary factors.

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Nuclear Magnetic Resonance Spectroscopic Based Studies of the Metabolism of Black Tea Polyphenols in Humans

Cited by 103 publications

References 34 publications

¹H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome

¹H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome

NMR‐based metabonomics reveals that plasma betaine increases upon intake of high‐fiber rye buns in hypercholesterolemic pigs

Assessment of dietary exposure related to dietary GI and fibre intake in a nutritional metabolomic study of human urine

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Nuclear Magnetic Resonance Spectroscopic Based Studies of the Metabolism of Black Tea Polyphenols in Humans

Cited by 103 publications

References 34 publications

1H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome

1H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome

NMR‐based metabonomics reveals that plasma betaine increases upon intake of high‐fiber rye buns in hypercholesterolemic pigs

Assessment of dietary exposure related to dietary GI and fibre intake in a nutritional metabolomic study of human urine

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¹H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome

¹H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome