Nuclear Magnetic Resonance Strategies for Metabolic Analysis

Heude, Clement; Nath, Jay; Carrigan, John B.; Ludwig, Christian

doi:10.1007/978-3-319-47656-8_3

Cited by 8 publications

(12 citation statements)

References 112 publications

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“…Antidepressant and anti‐seizure effects of citalopram have been demonstrated to be mediated through protein kinase C inhibition and Wingless‐Dishevelled‐glycogen synthase kinase 3β pathway in chronic forced swim stress, and pentylenetetrazole‐induced clonic seizure model, respectively (Augustyn et al, ; Liu et al, ; Payandemehr et al, ). Nuclear magnetic resonance spectroscopy offers a powerful, reproducible, and non‐destructive analytical tool for quantitative analyses and structural determination of metabolites (Heude, Nath, Carrigan, & Ludwig, ).…”

Section: Introductionmentioning

confidence: 99%

Citalopram attenuated neurobehavioral, biochemical, and metabolic alterations in transient middle cerebral artery occlusion model of stroke in male Wistar rats

Gupta

Upadhayay

Sharma

et al. 2018

J of Neuroscience Research

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Oxidative stress and inflammation are implicated as cardinal mechanisms of neuronal death following stroke. In the present study citalopram (Cit) was investigated in a 2 h middle cerebral artery occlusion (MCAo) model of stroke in male Wistar rats. Pretreatment, posttreatment (Post Cit) and pre plus posttreatment (Pre + Post Cit) with Cit were evaluated for its neuroprotective effect. In pretreatment protocol, effect of Cit at three doses (2, 4, and 8 mg/kg) administered i.p., 1 h prior to MCAo was evaluated using neurological deficit score (NDS), motor deficit paradigms, and cerebral infarction 24 h post-MCAo. In posttreatment and pre plus posttreatment protocol, the effective dose of Cit (4 mg/kg) was administered i.p., 0.5 h post-reperfusion (Post Cit) only, and 1 h prior to MCAo and again at 0.5 h post-reperfusion (Pre + Post Cit), respectively. These two groups were assessed for NDS and cerebral infarction. Though NDS was significantly reduced in both Post Cit and Pre + Post Cit groups, significant reduction in cerebral infarction was evident only in Pre + Post Cit group. Infarct volume assessed by magnetic resonance imaging was significantly attenuated in Pre + Post Cit group (10.6 ± 1.1%) compared to MCAo control group (18.5 ± 3.0%). Further, Pre + Post Cit treatment significantly altered 17 metabolites along with attenuation of malondialdehyde, reduced glutathione, matrix metalloproteinases, and apoptotic markers as compared to MCAo control. These results support the neuroprotective effect of Cit, mediated through amelioration of oxidative stress, inflammation, apoptosis, and altered metabolic profile.

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Section: Introductionmentioning

confidence: 99%

Citalopram attenuated neurobehavioral, biochemical, and metabolic alterations in transient middle cerebral artery occlusion model of stroke in male Wistar rats

Gupta

Upadhayay

Sharma

et al. 2018

J of Neuroscience Research

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“…Ex vivo 1H NMR spectroscopy of excised tissue generates spectra that enables separation and relative quantification of metabolites in the tissue. 42 In ex vivo, because the molecular structure of the sample is semi-solid, routine high resolution (HR) MRS of specimens might lead to peak broadening. The new solution is called the high-resolution magic angle spinning (HR-MAS) MRS. 43 In HR-MAS MRS, the data are collected by spinning the sample at a 54.7 rotation with respect to the static magnetic field.…”

Section: Ex Vivo Mrsmentioning

confidence: 99%

Magnetic resonance spectroscopy and its application in colorectal cancer diagnosis and screening: A narrative review

Zeinali-Rafsanjani

Jalli

Saeedi-Moghadam

et al. 2020

Journal of Medical Imaging and Radiation Sciences

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There are several slightly invasive methods to detect colorectal carcinoma (CRC) including colonoscopy and sigmoidoscopy; but there is no noninvasive, accurate screening test. It is recommended to initiate screening at the age of 50 for non-familial CRC. Laboratory tests are routinely suggested if internal observation and imaging are recommended for further evaluation. Spectroscopic-based imaging, such as magnetic resonance spectroscopy (MRS) is an interesting and promising tool with the potential to be an alternative to some minimally invasive procedures, such as biopsy. Accordingly, MRS might be a suitable substitution for invasive methods, such as colonoscopy. This article aimed to review the studies that have evaluated the MRS technique as a screening tool in CRC. R ESUM E Il existe plusieurs m ethodes minimalement invasives pour la d etection de carcinome colorectal (CRC), incluant la colonoscopie et la sigmo€ ıdoscopie; mais il n'existe aucun test de d epistage qui soit a la fois non invasif et pr ecis. Il est recommand e d'entreprendre le d epistage a l'âge de 50 ans pour les patients sans incidence familiale de carcinome colorectal. Les tests de laboratoire sont recommand es de façon routini ere si l'observation interne et l'imagerie sont n ecessaires pour une evaluation plus pouss ee. L'imagerie bas ee sur la spectroscopie, par exemple la spectroscopie par r esonance magn etique (SRM), est un outil int eressant et prometteur ayant le potentiel d'offrir une solution de rechange a certaines proc edures minimalement invasives, comme la biopsie. De la même façon, la SRM pourrait remplacer de façon appropri ee des m ethodes et des proc edures plus invasives, comme la colonoscopie. Cet article se penche sur les etudes qui ont evalu e la technique de SRM comme outil de d epistage pour le carcinome colorectal.

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“…NMR has been an extensively applied method in metabolomics analysis. 16 This technique may be particularly effective when the effects and mechanisms of drugs on organisms are unknown.…”

Section: Introductionmentioning

confidence: 99%

Astragaloside IV Extends Lifespan ofCaenorhabditis elegansby Improving Age-Related Functional Declines and Triggering Antioxidant Responses

Zhang

Xue

Qiao

et al. 2021

Rejuvenation Research

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Astragaloside IV (AS-IV) is a representative component of astragaloside saponins in dried roots of Astragali Radix. Astragaloside possesses a broad spectrum of pharmacological activities, including antibacterial, antifibrosis, antioxidant, anti-inflammatory, and neuroprotective effects. However, the role of AS-IV in antiaging remains unclear. In this article, we studied the function of AS-IV in antiaging by using the Caenorhabditis elegans (C. elegans) model. We showed that AS-IV can prolong the lifespan of C. elegans in a natural aging model, a paraquat injury model, and a heat stress model and improve the movement capacity of nematodes. 1 H-NMR data indicate an improvement of glutamate content and a decrease in glucose in the AS-IV treatment group compared with the control. Further investigation revealed that AS-IV can induce the mRNA expression of superoxide dismutase (SOD) and catalase (CAT) genes and increase the activities of SOD and CAT in the nematode. Interestingly, AS-IV could not extend the lifespan of sod-1, sod-2, sod-3, sod-4, sod-5, ctl-1, ctl-2, ctl-3, and daf-16 mutants. These data indicate that AS-IV prevents aging via mainly improving age-related functional declines, the antioxidant capacity of nematodes and partially modulating the insulin/insulin growth factor 1 signaling pathway activity. Our results provide new insights into how AS-IV prevents and treats aging.

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Nuclear Magnetic Resonance Strategies for Metabolic Analysis

Cited by 8 publications

References 112 publications

Citalopram attenuated neurobehavioral, biochemical, and metabolic alterations in transient middle cerebral artery occlusion model of stroke in male Wistar rats

Citalopram attenuated neurobehavioral, biochemical, and metabolic alterations in transient middle cerebral artery occlusion model of stroke in male Wistar rats

Magnetic resonance spectroscopy and its application in colorectal cancer diagnosis and screening: A narrative review

Astragaloside IV Extends Lifespan ofCaenorhabditis elegansby Improving Age-Related Functional Declines and Triggering Antioxidant Responses

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