Nuclear maturation and embryo development of porcine oocytes vitrified by cryotop: Effect of different stages of in vitro maturation

Wu, Guoquan; Jia, Baoyu; Mo, Xianhong; Liu, Cong; Fu, Xiangwei; Zhu, Shi-En; Hou, Yunpeng

doi:10.1016/j.cryobiol.2013.05.010

Cited by 18 publications

(14 citation statements)

References 52 publications

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“…Furthermore, it has been demonstrated that combinations of different CPAs give better results than the use of a single type of CPA in pigs [ 6 ] as well as in other mammalian species [ 7 ]. To date, three main vitrification techniques have been applied to porcine oocytes with various results: the SSV [ 5 , 6 , 8 , 9 , 10 , 11 , 12 ], Cryotop [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ] and Open Pulled Straw (OPS) [ 15 , 18 , 21 , 22 , 23 , 24 , 25 ] methods. Although the main difference among these techniques is the cryodevice (i.e., the carrier that gives the distinctive name to each method), these methods also differ in terms of the CPA treatment including the type of sugars and permeating CPA, their concentrations during equilibration and the duration of equilibration ( Table 1…”

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“… [ 19 ] Cryotop 1 EG (4%) 15 min EG (35%) Trehalose (0.4 M) 30–60 sec GV: 4% [ 18 ] Cryotop 2 EG (7.5 → 15%) 1–4 min EG (30–35%) Sucrose (0.5–0.6 M) 30–60 sec GV: 40% (approx.) [ 13 , 16 , 20 ] MII: 80% (approx.) OPS 1 EG (10%) + DMSO (10%) 3 (5)–10 min EG (20%) + DMSO (20%) Sucrose (0.6 M) 30–50 sec MII: 68.2% [ 15 , 23 , 24 ] OPS 6 EG (5→10→15→20→25→30%) 12.5 min EG (40%) None 25 sec No data [ 21 ] OPS 1 EG (10%) + DMSO (10%) 90 sec EG (20%) + DMSO (20%) …”

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confidence: 99%

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Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration regimens

Somfai

Men

Noguchi

et al. 2015

Journal of Reproduction and Development

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Our aim was to optimize the cryoprotectant treatment for the preservation of immature porcine cumulus-oocyte complexes (COCs) by solid surface vitrification. In each experiment, the vitrification solution consisted of 50 mg/ml polyvinyl pyrrolidone, 0.3 M of the actual sugar and in total 35% (v/v) of the actual permeating cryoprotectant (pCPA) combination. After warming, the COCs were subjected to in vitro maturation, fertilization and embryo culture. In Experiment 1, trehalose and sucrose were equally effective during vitrification and warming in terms of facilitating oocyte survival and subsequent embryo development. In Experiment 2, when equilibration was performed at 38.5 C in a total of 4% (v/v) pCPA for 15 min, the combination of ethylene glycol and propylene glycol (EG + PG = 1:1) was superior to EG and dimethyl sulfoxide (EG + DMSO = 1:1) in terms of oocyte survival after vitrification and the quality of resultant blastocysts. In Experiment 3, equilibration in 4% (v/v) pCPA for 15 min before vitrification was superior to that in 15% (v/v) CPA for 5 min for achievement of high survival rates irrespective of the pCPA combination used. In Experiment 4, when equilibration was performed in 4% EG + PG for 5 min, 15 min or 25 min, there was no difference in oocyte survival and subsequent embryo development after vitrification and warming; however, the developmental competence of cleaved embryos was tendentiously reduced when equilibration was performed for 25 min. In conclusion, trehalose and sucrose were equally effective in facilitating vitrification, and the optimum pCPA treatment was 5–15 min equilibration in 4% (v/v) of EG + PG followed by vitrification in 35% (v/v) EG + PG.

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confidence: 99%

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confidence: 99%

Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration regimens

Somfai

Men

Noguchi

et al. 2015

Journal of Reproduction and Development

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“…After resuspension in prewarmed Tyrode's medium (TLH) containing 0.1% (w/v) polyvinyl alcohol (PVA; TLH‐PVA; Funahashi, Cantley, & Day, 1997), oocytes with uniform cytoplasm and compact cumulus cells were selected under a stereo microscope (SZ61; Olympus, Tokyo, Japan) for maturation. Groups of about 50 COCs were cultured in 500 μl of tissue culture medium 199 with Earle's salts (TCM199; Gibco, Grand Island, NY) supplemented with 0.57 mM cysteine, 0.91 mM sodium pyruvate, 10 ng/ml EGF, 0.01 U/ml FSH (Sioux Biochemical, Sioux Center, IA), 0.01 U/ml LH (Sioux Biochemical) and 10% (v/v) porcine follicular fluid (Yuan & Krisher, 2010) at 38.5°C in 5% CO 2 and saturated humidity atmosphere for 42–44 hr (Wu et al, 2013). Matured oocytes were denuded in TLH‐PVA containing 0.1% (w/v) hyaluronidase.…”

Section: Methodsmentioning

confidence: 99%

The extracellular calcium‐sensing receptor promotes porcine egg activation via calcium/calmodulin‐dependent protein kinase II

Liu

Luo

et al. 2020

Molecular Reproduction Devel

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Extracellular calcium is required for intracellular Ca 2+ oscillations needed for egg activation, but the regulatory mechanism is still poorly understood. The present study was designed to demonstrate the function of calcium-sensing receptor (CASR), which could recognize extracellular calcium as first messenger, during porcine egg activation. CASR expression was markedly upregulated following egg activation. Functionally, the addition of CASR agonist NPS R-568 significantly enhanced pronuclear formation rate, while supplementation of CASR antagonist NPS2390 compromised egg activation. There was no change in NPS R-568 group compared with control group when the egg activation was performed without extracellular calcium addition. The addition of NPS2390 precluded the activation-dependent [Ca 2+ ] i rise. When egg activation was conducted in intracellular Ca 2+ chelator BAPTA-AM and NPS R-568 containing medium, CASR function was abolished. Meanwhile, CASR activation increased the level of the [Ca 2+ ] i effector p-CAMKII, and the presence of KN-93, an inhibitor of CAMKII, significantly reduced the CASR-mediated increasement of pronuclear formation rate. Furthermore, the increase of CASR expression following activation was reversed by inhibiting CAMKII activity, supporting a positive feedback loop between CAMKII and CASR. Altogether, these findings provide a new pathway of egg activation about CASR, as the extracellular Ca 2+ effector, promotes egg activation via its downstream effector and upstream regulator CAMKII.

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“…CPAs function in a dual capacity by decreasing freezing temperature and increasing viscosity so that instead of crystallizing the syrupy solution becomes an amorphous ice; it 'vitrifies' . While conventional cryoprotectants, such as glycols and dimethyl sulfoxide, are intrinsically toxic to mammalian cells [14,15], Kuwayama [16] has argued that vitrification does not require high concentrations of CPAs such that under special conditions in which the cooling rate is equal to or greater than 107 o C/s [16,17], it is possible to induce vitrification with pure water (zero CPAs). Seki et al [18] also contend that there is an incorrect belief that vitrification is achieved only when cells are exposed to both high CPA levels and rapid cooling rates (>> 10,000 o C/min).…”

Section: Vitrification: High Warming and Cooling Rate Methodsmentioning

confidence: 99%

Benefits and Constraints of Vitrification Technologies for Cryopreservation of Bovine in vitro Fertilized Embryos

Vh¹,

Walton²,

Aw³

2014

Journal of Veterinary Science and Animal Husbandry

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Nuclear maturation and embryo development of porcine oocytes vitrified by cryotop: Effect of different stages of in vitro maturation

Cited by 18 publications

References 52 publications

Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration regimens

Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration regimens

The extracellular calcium‐sensing receptor promotes porcine egg activation via calcium/calmodulin‐dependent protein kinase II

Benefits and Constraints of Vitrification Technologies for Cryopreservation of Bovine in vitro Fertilized Embryos

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