Nuclear phenotypes and morphometry of human secretory prostate cells: a comparative study of benign and malignant lesions in Brazilian patients

Taboga, Sebastião Roberto; Santos, Adriana Barbosa; Gonzatti, Adriana G. R.; Vidal, Benedicto de Campos; Mello, Maria Luiza S.

doi:10.1080/00087114.2003.10589340

Cited by 18 publications

(21 citation statements)

References 12 publications

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“…Additionally, this type of isolated quantitative analysis had not been effective in aiding the diagnosis of prostate lesions in old gerbils. In human prostate diseases, nuclear parameters were also ineffective in differentiating adenocarcinomas of different Gleason degrees, but they were efficient in comparing BPH and adenocarcinoma (Martínez-Jabaloyas et al 2002;Taboga et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Nuclear areas, perimeters and the form factor [=4π.nuclear area/(nuclear perimeter) 2 ] parameter were determined for 200 nuclei of the epithelial secretory cells in each group, that is: non‐affected, PIN, microinvasive carcinoma and HSA. The form factor parameter measures nuclear roundness and values <1 are associated with nuclei which are less round (Taboga et al. 2003).…”

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confidence: 99%

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Age‐related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone‐related disorders

Campos

Zanetoni

Scarano

et al. 2007

Int J Experimental Path

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The Meriones unguiculatus (Mongolian) gerbil has demonstrated significant prostatic responses to hormonal treatments, and to drugs against human prostatic hyperplasia. Spontaneous neoplasia develops in the older animals. Thirty gerbils (age 18 months) were divided into non-affected and prostatic lesion bearers and the prostate lesions were evaluated morphologically, immunohistochemically and quantitatively. The most frequent changes were in epithelial sites and, namely prostatic intraepithelial neoplasias, microinvasive carcinomas and adenocarcinomas. In the stromal compartment, cellular hyperplasia, when verified, was always associated with the sites of anomalous epithelium. Additionally, larger deposition of collagen fibrils, generating stromal fibrosis, was found in all the old gerbils analysed. The quantitative analysis showed that prostatic tissue proportions differed in altered areas, being specific for each lesion type. Isolated nuclear and nucleolar parameters were not effective in diagnosing the malign potential of lesions. However, the cellular proliferation and death indexes indicated larger cellular turnover in invasive lesions such as carcinomas. With these analyses, it could be verified that old gerbils present high propensity to develop spontaneous prostate changes and this may aid in a better understanding of the biological behaviour of human prostate cancer.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Age‐related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone‐related disorders

Campos

Zanetoni

Scarano

et al. 2007

Int J Experimental Path

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“…Nuclear parameters can be efficient to compare some epithelial modifications (Martínez‐Jabaloyas et al. 2002; Taboga et al. 2003), but it is not effective in diagnosis of prostate lesions and should be interpreted together with other results.…”

Section: Discussionmentioning

confidence: 99%

“…The kariometric data revealed that even the E and CaE groups had increased the nuclear area, besides an increase of the epithelial height, as was previously observed by Scarano et al (2004) and could be related with increase of the synthetic and ⁄ or metabolic cell activity. Nuclear parameters can be efficient to compare some epithelial modifications (Martínez-Jabaloyas et al 2002;Taboga et al 2003), but it is not effective in diagnosis of prostate lesions and should be interpreted together with other results. Recent research employing 40 quantitative nuclear morphometric parameters suggested with those parameters as a new biomarker for prostatic cancer and histopathological gradation (Veltri et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Oestrogen supplementation following castration promotes stromal remodelling and histopathological alterations in the Mongolian gerbil ventral prostate

Scarano

Sousa

Campos

et al. 2007

Int J Experimental Path

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The effect of oestradiol on the intact and castrated adult gerbil prostate was evaluated by focussing on stromal and epithelial disorders, and hormonal receptor immunoreactivity. The experimental animals were studied by histological, histochemical and immunohistochemical techniques, morphometric-stereological analysis and transmission electron microscopy. Epithelial alterations in the oestradiol-treated animals were frequent, with an increase in epithelial cell height, areas of intense dysplasia and hyperplasia and formation of prostatic intraepithelial neoplasia (PIN). Another aspect that did not depend on the presence of testosterone was the arrangement of the fibrillar and non-fibrillar elements of the extracellular matrix among smooth muscle cells (SMC), suggesting a possible role of these cells in rearrangement and synthesis of these components, after oestrogenic treatment. In the castrated animals, an accumulation of extracellular matrix elements under the epithelium was evident, while in the intact animals the same compounds were dispersed and scarce. In the groups of intact and castrated animals, SMC and fibroblasts exhibited a secretory phenotype, which was accentuated after oestradiol administration. There was an increase of the immunoreactivity to alpha-oestrogen and androgen receptors in hyperplastic areas compared to normal epithelium, revealing the involvement of these steroid receptors in the hyperplasia and PIN development.

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“…For a perfect circle, the form factor is equal to one and for shapes that deviate from the circle, the values are smaller than one (Taboga et al 2003).…”

Section: Seasonal Evaluationmentioning

confidence: 99%

Structure, histochemistry and seasonal variations of the male reproductive accessory glands in the Pallas’s mastiff bat, Molossus molossus (Chiroptera: Molossidae)

Christante

Beguelini

Puga

et al. 2015

Reprod. Fertil. Dev.

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Due to their wide geographical distribution, bats suffer considerable influence from abiotic factors on their reproductive strategies, detected through behavioural or functional assessment of the gonads and accessory glands. The present study aimed to characterise anatomically and morphologically the reproductive accessory glands (RAGs) of Molossus molossus (Molossidae) and evaluate their seasonal variations. The RAGs were removed, fixed, sectioned after histological processing and submitted to the following stains: haematoxylin-eosin, periodic acid--Schiff (PAS) and Gömöri's reticulin. Our data demonstrated that the RAGs of M. molossus are composed of a semi-lobed prostatic complex associated with the urethra and a pair of inguinal bulbourethral glands. Histology and three-dimensional reconstruction of the prostatic complex demonstrated the existence of two regions: ventral and dorsal (named according to the position around the urethra). The two regions had distinctive morphological and histological differences, with the ventral region being the most prominent and the dorsal formed by four lobes. Considering the seasonal evaluation, we can infer that the prostatic complex of M. molossus is active throughout the seasons in São Paulo State (Brazil) and, although each of the prostatic regions has inherent characteristics, they synchronise to establish the main reproductive peak in spring (early rainy season).

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Nuclear phenotypes and morphometry of human secretory prostate cells: a comparative study of benign and malignant lesions in Brazilian patients

Cited by 18 publications

References 12 publications

Age‐related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone‐related disorders

Age‐related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone‐related disorders

Oestrogen supplementation following castration promotes stromal remodelling and histopathological alterations in the Mongolian gerbil ventral prostate

Structure, histochemistry and seasonal variations of the male reproductive accessory glands in the Pallas’s mastiff bat, Molossus molossus (Chiroptera: Molossidae)

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