Nuclear Ribosomal Dna Variation Among Ramets and Genets of White Clover

Capossela, Angela; Silander, John A.; Jansen, Robert K.; Bergen, Briana; Talbot, Duncan R.

doi:10.1111/j.1558-5646.1992.tb00634.x

Cited by 11 publications

(1 citation statement)

References 42 publications

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“…The analysis of variability within genes encoding rRNA subunits (rDNA), that include ITS (internal transcribed spacers), IGS (intergenic sparcer) and sequences of rRNA genes: SSU (small subunit), 5.8S and LSU (large subunit) is one of many ways of determining the phylogenetic relationships between accessions (Pillay and Kenny, 1996;Smolik et al, 2009;Poczai and Hyvönen, 2010). Variation in the rDNA repeats has been useful in addressing questions in population genetics (Capossela et al, 1992). It is also considered a useful genetic marker for breeding applications (Poczai and Hyvönen, 2010).…”

Section: Introductionmentioning

confidence: 99%

rDNA Variability Assessed in PCR Reactions of Selected Accessions of Acer

Smolik

Krupa-Małkiewicz

Smolik

et al. 2011

Not Bot Hort Agrobot Cluj

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The region of rDNA (SSU, 5.8S, LSU and ITS1, ITS2 spacer sequences) of fourteen Acer accessions was tested in order to determine the possibility to identify genotypic variability within a rDNA sequence. The tests were performed using the PCR technique and a set of combination of several pairs of ‘universal’ primers designed to amplify rDNA sequences. ‘Universal’ primers were selected in such a way as to make the rDNA regions flanking possible in cases where a smaller or larger scope of variability was expected. Thus, within SSU or LSU sequences, monomorphic PCR products were amplified for the tested Acer accessions. Within the ITS sequence, a greater scope of variability was found. This was manifested by the presence of several additional PCR products in the genetic profiles of the tested Acer accessions. The range of their length relative to the ITS region or ITS1, ITS2 or 5.8S corresponded separately to that described in the literature. The analysis of the topology of the constructed tree revealed the presence of two similar groups, ‘a’ and ‘b’. Cultivated varieties and one botanical variety of the Japanese maple were included respectively in these groups and the genetic similarity between the analyzed accessions ranged from 63 to 98%.

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Section: Introductionmentioning

confidence: 99%

rDNA Variability Assessed in PCR Reactions of Selected Accessions of Acer

Smolik

Krupa-Małkiewicz

Smolik

et al. 2011

Not Bot Hort Agrobot Cluj

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Plant Breeding: Clonality — A Concept for Stability and Variability During Vegetative Propagation

Forneck

Progress in Botany

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Structure and inheritance of ribosomal DNA variants in cultivated and wild hop, Humulus lupulus L.

Pillay

Kenny

1996

Theoret. Appl. Genetics

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Genetic variation was assessed among cultivated and wild hop, Humulus lupulus, by restriction fragment length polymorphisms (RFLPs) of the ribosomal RNA genes (rDNA). Two rDNA length variants of 10.3 and 9.3 kbp represented by three phenotypes designated A, B and C were detected with XhoI. Restriction-site mapping showed that hop rDNA is structurally similar to those of most higher plants. A high level of homogeneity existed in rDNA repeat lengths among the diverse hop genotypes. Generally, phenotype A was predominant in wild and cultivated European and Asian genotypes; phenotype B in North American cultivars; while phenotype C was present only in native North American hop, providing a potential molecular marker for the identification of this germ plasm. The rDNA data provided genetic evidence for the separation of native and cultivated American genotypes and supports the hypothesis that North American hop cultivars are of hybrid origin from European and native American genotypes. The segregation of rDNA phenotypes in four F1 families suggests that a single locus with two co-dominant alleles controls genetic variability for rDNA variants in hop.

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Nuclear Ribosomal Dna Variation Among Ramets and Genets of White Clover

Cited by 11 publications

References 42 publications

rDNA Variability Assessed in PCR Reactions of Selected Accessions of Acer

rDNA Variability Assessed in PCR Reactions of Selected Accessions of Acer

Plant Breeding: Clonality — A Concept for Stability and Variability During Vegetative Propagation

Structure and inheritance of ribosomal DNA variants in cultivated and wild hop, Humulus lupulus L.

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