Nuclear shape and DNA content of fully habituated nonorganogenic sugarbeet cells

Hagège, Daniel; Catania, Rosa; Micalef, H.; Gaspar, Thomas

doi:10.1007/bf01320142

Cited by 17 publications

(5 citation statements)

References 10 publications

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“…Structural and biochemical characteristics of the N and HNO cells have already been intensively studied (Gaspar et al , 2000Hagège et al 1992;Gaspar 1995Gaspar , 1999, and HNO cells have been shown to exhibit many characteristics of cancer cells Gaspar 1999). The present study of HNO cells showed enlarged, lobed nuclei with an increased number of nucleoli and undeveloped vacuolated plastids, with reduced thylakoid membranes that were occasionally arranged concentrically.…”

Section: Discussionmentioning

confidence: 98%

“…vulgaris var. altissima) have been intensively studied at the biochemical, morphological, and physiological levels (Crèvecoeur et al 1987(Crèvecoeur et al , 1992Hagège et al 1992;Liners et al 1994;Kevers et al 1999;Häsler et al 2003). These have been recommended as a model for studying plant cell differentiation (Gaspar 1999;Gaspar et al 2000;Causevic et al 2005).…”

Section: Introductionmentioning

confidence: 98%

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Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Pavoković

Poljuha²,

Horvatić

et al. 2011

Plant Cell Tiss Organ Cult

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Normal (N), habituated nonorganogenic (HNO), and tumour (T) sugar beet cell lines were analysed in order to establish specific patterns of extracellular proteins and identify protein markers that might explain the distinct phenotypical characteristics. Electron microscopy showed that the ultrastructure of N cells corresponds to that of parenchyma cells, and that these cells contain plastids with large starch grains. HNO and T cells had enlarged, lobed nuclei with an increased number of nucleoli; the number of nuclei in HNO cells was greater than in T cells. The T plastids were elongated, with reduced thylakoids and abundant phytoferritin deposits, while HNO plastids were small and vacuolated, with an irregular, underdeveloped thylakoid system. The extracellular proteome of the cells was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Greater differences in protein expression were observed between the HNO and N lines than between the T and N lines. Sixteen of the most prominent bands differentially expressed among the cell lines were cut out from the gel and analyzed by mass spectrometry. Cell wall-modifying enzymes were identified, including a peroxidase whose expression was twofold higher in N and T tissue than in HNO tissue; pectinesterase, which was expressed at a level threefold lower in the T line than in the other cell lines; and xyloglucan endotransglucosylase, which was expressed at a level sixfold higher in HNO and T tissue. Three proteins belonged to the chitinase gene family and their expression was higher in HNO and T tissue than in N tissue. The differential expression of these proteins suggests that these play a role in cell line-specific cell wall composition and cell-to-cell adhesion.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Pavoković

Poljuha²,

Horvatić

et al. 2011

Plant Cell Tiss Organ Cult

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“…Micronuclei were observed in approximately 30% of the cells (results not presented). These features suggest that such cells were derived from a tissue that was already dividing in vivo (Hagège et al, 1992;Ramulu et al, 1993) Furthermore, the observed changes in nuclear structure of the totipotent protoplast type, prior to cell division, would clearly support this conclusion. With the progenitors of compact callus also being cytoplasm rich and poorly vacuolated and having no chloroplasts, our present working hypothesis is that they are derived from cambial initials.…”

Section: Discussionmentioning

confidence: 69%

Computer-Assisted Identification of Protoplasts Responsible for Rare Division Events Reveals Guard-Cell Totipotency

1995

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“…Therefore, the high level of H20 2 which generates the high toxic radicals OH", via the Fenton reaction, may explain the lipoperoxidation of cellular structures and the vitrified status of the H callus. On another hand the abnormal nuclear structure (Hag6ge et al 1992a) may be the result of DNA damages (Ramasarma 1990) provoked by the high H202 level. The deficiency in cell-wall structure, which is a polycationic accumulator, is probably due to the lack of polymerizing enzymes, and especially of syringaldazine peroxidases (Cr6vecoeur et al 1987).…”

Section: Resultsmentioning

confidence: 99%

Does altered nitrogen metabolism and H2O2 accumulation explain the vitrified status of the fully habituated callus of Beta vulgaris (L.)?

Dily

Huault

Gaspar

et al. 1993

Plant Cell Tiss Organ Cult

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The habituated callus is a vitrified tissue which has two main biochemical characteristics both leading to production of toxic forms of oxygen: first the blockage of the porphyrin pathway and a lack of H20 2 detoxifying enzymes (catalase and peroxidases); secondly a deviation of the nitrogen metabolism induced by NH 3 accumulation. Ammonia detoxification is ensured by increased glutamate dehydrogenase activity and accumulation of both proline and polyamines. A putative linkage between proline synthesis and the HMP pathway, as proposed for animal proliferating tissues (Phang 1985), might explain a high purine biosynthesis and cytokinin autonomy.Abbreviations: FFA-free fatty acids, 6PG-DH-6-phosphogluconate dehydrogenase, G6P-DH-

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Nuclear shape and DNA content of fully habituated nonorganogenic sugarbeet cells

Cited by 17 publications

References 10 publications

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Computer-Assisted Identification of Protoplasts Responsible for Rare Division Events Reveals Guard-Cell Totipotency

Does altered nitrogen metabolism and H2O2 accumulation explain the vitrified status of the fully habituated callus of Beta vulgaris (L.)?

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