Nuclear spin selectivity in enzymatic catalysis: A caution for applied biophysics

Oncogenesis and Aging by Isotopic Functionalizations of the Proteins and Nucleic Acids

“…Moreover isotopic replacements and clumping in cancer cells are shown to alter functionalizations of nucleosides, nucleotides, oligonucleotides and the telomeric gene by altering patterns of methylation, acetylation, amination, hydroxylation and phosphorylation of DNA in cancer cells relative to DNA in normal cells for causing altered upregulations, downregulations and mutations for different properties of the cancer DNA relative to the normal DNA.On this basis, our work provides further confirmation of recent theory [4,5] and the observed selective sensitivity of cancer cells to the nonprimordial isotopes: 25 Mg, 43 Ca and 67 Zn versus normal cells sensitivity. This may explain also the lack of sensitivity to the nonprimordial isotopes of 24 Mg, 40 Ca, and 64 Zn of cancer and normal cells [6]. Additionally, this work may explain recent twin experiments of NASA [7] where telomeres of prolonged, space orbiting twin brother were elongated relatively to earth bound twin brother.…”

“…On this basis, our work provides further confirmation of recent theory [4,5] and the observed selective sensitivity of cancer cells to the nonprimordial isotopes: 25 Mg, 43 Ca and 67 Zn versus normal cells sensitivity. This may explain also the lack of sensitivity to the nonprimordial isotopes of 24 Mg, 40 Ca, and 64 Zn of cancer and normal cells [6]. Additionally, this work may explain recent twin experiments of NASA [7] where telomeres of prolonged, space orbiting twin brother were elongated relatively to earth bound twin brother.…”

A New DNA Repair-Related Platform for Pharmaceutical Outlook in Cancer Therapies: Ultrashort Single-Stranded Polynucleotides

“…A background-lying platform of this work derives from experimental data on magnetic isotope effects (MIE) towards both DNApolβ catalytic activity [1][2][3] and the viability of cancer cells [1,2]. Thus, a sharp decrease of the survival ability patterns of 25 Mg 2+ -treated AML/HL-60 cells as compared to abundant spineless, non-magnetic magnesium ions impact was found [1].…”

“…A background-lying platform of this work derives from experimental data on magnetic isotope effects (MIE) towards both DNApolβ catalytic activity [1][2][3] and the viability of cancer cells [1,2]. Thus, a sharp decrease of the survival ability patterns of 25 Mg 2+ -treated AML/HL-60 cells as compared to abundant spineless, non-magnetic magnesium ions impact was found [1]. Furthermore, a similar result was then obtained from the same leukemia cells treated with magnetic, nuclear spin possessing, 43 Ca 2+ and 67 Zn 2+ ions [1,3] as well as on human retinoblastoma cells subjected to these metal isotopes [2,3].…”

“…It was also shown that a processivity of beta-like DNA polymerases isolated from the all abovementioned malignant cells depends on MIE, so the resulted DNA fragment sizes becomes shorter within a 40-250 n range following the increase of magnetic isotope content in a total metal pool [2,3]. For instance, an up to 58% elevation of 43 Ca 2+ content leads to a monotonic decrease of sizes of polynucleotides processed from average 230-250 n to an abnormal (DNA repair invalid) 36-40 n as directed by beta-like DNA polymerases from Y-79 and WERI-1A retinoblastoma cells [1].…”