Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line

Yatouji, Sonia; Trussardi‐Régnier, Aurélie; Trentesaux, Chantal; Liautaud-Roger, F; Dufer, Jean

doi:10.3892/ijo.23.4.1225

Cited by 9 publications

(22 citation statements)

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“…In addition, the nuclear phenotype observed in the GC-resistant RPMI 8226 cells is totally different from the phenotype displayed by MDR cells, where an increased chromatin decondensation and the appearance of a more fine and homogenous texture, underlining a different distribution of chromatin displayed in all cell lines tested so far. 13,14,24 At the GR level, previous data showed GRβ expression was not selectively stimulated in 8226 resistant cells. On the contrary, the resistance was associated with an upregulation of all the GR isoforms, including GRα.…”

Section: Discussionmentioning

confidence: 97%

“…We and others have reported previously specific nuclear textural changes in various multidrug-resistant cell lines. [13][14][15][24][25][26] These data concerned the well documented MDR phenomenon but there is, up to now, no information available on such alterations in glucocorticoid resistant cell lines. Hence, we analyzed the nuclear textural phenotype of 3 RPMI 8226 myeloma cell sub-lines resistant to glucocorticoids.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, texture analysis of cell nuclei enabled tumor diagnosis and grading, 11,12 but also characterization of cell subsets, as drug-sensitive or -multidrug resistant (MDR) cell lines 13 where MDR cells displayed specific textural features and chromatin decondensation. The biological grounds of these morphological changes remain largely unknown but appear related to chromatin structure 14,15 and could be implicated in cellular functions as it is evident that higher order chromatin organization plays an important role in nuclear function. 16 From this point of view, it appeared interesting to look for specific chromatin organization changes associated with GC-resistance.…”

Section: Introductionmentioning

confidence: 99%

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Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines: Correlations with cell growth and immunological phenotype

Genty

El-Khoury

Liautaud-Roger

et al. 2005

Cancer Biology & Therapy

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines: Correlations with cell growth and immunological phenotype

Genty

El-Khoury

Liautaud-Roger

et al. 2005

Cancer Biology & Therapy

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“…Nuclear texture is a reflection of the overall structure of the nuclear chromatin (5,20). Studies of nuclear texture features have proved to be useful for the development of treatment protocols or to be effective as markers for cancer screening (21).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, in cell lines derived from various tissues (lung, ovary, breast), we and others reported that this drug resistance was associated with alterations in nuclear architecture that could impact epigenetic ABCB1 gene control (2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Thalidomide alters nuclear architecture without ABCB1 gene modulation in drug-resistant myeloma cells

Trussardi-Regnier¹

2009

Int J Oncol

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Abstract. ABCB1 gene overexpression has been described as an important mechanism for resistance to conventional chemotherapy in multiple myelomas. In the refractory multiple myelomas, other drug regimens have been successfully applied, including thalidomide treatments. Besides its well-documented anti-angiogenic effects, thalidomide therapy could result in a decrease in ABCB1 gene expression. In this study, we analysed the effects of a 24-h short-term treatment by thalidomide or its active metabolite phthaloyl glutamic acid (PGA) on nuclear chromatin higher-order organisation and ABCB1 gene expression in drug-sensitive and drug-resistant 8226 human myeloma cells. As compared to sensitive cells, 8226-Dox40 drug-resistant cells exhibited an increase in chromatin texture condensation and ABCB1 gene overexpression. At this gene promoter level, the -50 and -100 GC boxes displayed an unmethylated profile in drug-sensitive cells whereas drug-resistant cell promoter GC boxes were fully methylated. Thalidomide and PGA induced significant chromatin textural changes in 8226-Dox40 drug-resistant cells only with neither alteration in ABCB1 gene expression nor methylation profile of its promoter. Conversely thalidomide and PGA induced down-regulation of VEGF gene expression in both drug-sensitive and -resistant myeloma cells. These data suggest that short-term treatments by thalidomide or PGA do not induce any significant change on ABCB1 gene expression though they modulate chromatin supra-organisation in drug-resistant 8226 human myeloma cells.

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Effects of the histone deacetylase inhibitor trichostatin A on nuclear texture and c‐jun gene expression in drug‐sensitive and drug‐resistant human H69 lung carcinoma cells

El-Khoury¹,

Gómez²,

Liautaud-Roger

et al. 2004

Cytometry Pt A

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Background: Texture analysis of chromatin patterns by image cytometry can be used in the development and refinement of diagnosis and prognosis of cancers and in the follow-up of therapies. However, little is known about the biological mechanisms underlying these patterns. Epigenetic mechanisms as histone posttranslational modifications and particularly histone acetylation could play a major role in the determination of these chromatin patterns and then influence nuclear texture measurements. Methods: This study examined the consequences of treatment by the histone deacetylase inhibitor trichostatin A (TSA) on the nuclear texture in human cell lines sensitive and resistant to chemotherapy. Small cell lung carcinoma H69 cells and their variant H69-VP, which is resistant to etoposide, were incubated with 100 ng/ml of TSA for 0 to 24 h. Nuclear texture was evaluated by image cytometry and compared with the histone H4 acetylation level measured by western blotting and expression of c-jun gene evaluated by reverse transcription and real-time polymerase chain reaction. Results: TSA treatment induced an increase in histone H4 acetylation level in both cell lines. However, at the level of

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Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line

Cited by 9 publications

References 0 publications

Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines: Correlations with cell growth and immunological phenotype

Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines: Correlations with cell growth and immunological phenotype

Thalidomide alters nuclear architecture without ABCB1 gene modulation in drug-resistant myeloma cells

Effects of the histone deacetylase inhibitor trichostatin A on nuclear texture and c‐jun gene expression in drug‐sensitive and drug‐resistant human H69 lung carcinoma cells

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